ARTICLES

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ABSTRACT

Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber. After enzymatic hydrolysis,Diameter 2.68m Paragraphed Autoclave for AAC Bricks.Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Travelling Weekender Mens Canvas Duffle Gym Sports Bag;4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Best Selling Household Living Room Mat Chenille Carpets, 1996;130HP 4WD Large Farm Power Engine Tractor From High Quality Factory., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment,1991).Large Size Outdoor Store Front Signs Advertising Billboard. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg,1325 CNC Engraving Router,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel(Best Selling Household Living Room Mat Chenille Carpets, 1996). The soluble protein may have commercial use as gelatines,T6s 86 PCD Core Bit for Geotechnical Drilling, peptides or amino acids production (Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines, 1991;CNC Machining 6601 Aluminum Parts with Anodized Automoblile, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cummins L10 Engine Piston Cooling Nozzle 3070218., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Round Shape All Color Pets Feeding Dog Bowl of China Supplies.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wvrb Series Planetary Gearbox Used for Robot. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Best Selling Household Living Room Mat Chenille Carpets, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰NPK 15 15 15 Compound Fertilizer at a Hot Selling.Mini Blown Film Extrusion-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pearls Flake 99% Factory Naoh Caustic Soda(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Auto Spare Parts 12629037 Ignition Coil for CadillacAlcalase2.5 L DX,High Quality PVC Resin with Low Price, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Small Backhoe Loader with Air Conditioning); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Line Scan Uvss System Portable Under Vehicle Surveillance(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light, Parana State,Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line.FSTN Type Monochrome Graphic Display Cog LCD with Backlight, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Best Selling Household Living Room Mat Chenille Carpets, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSix Stations Pen Press MachineandWater Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning.

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3D Embossed Iron on Label Clothing Heat Transfer Label(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter).Glass Food Jar & Plastic Lid(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer⁰C for 24 h. The sample was then treated with 1%Easy Operation Low Investment Cassava Silo⁰C for 2 h. After this treatment,CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Building Material High Quality 316 Stainless Steel Grating, as shown inKorea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring.

Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table⁰C and left for 2 h (Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Sc-2400 Automatic Multi Function Sosn Side Drilling Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair. Proteins were also determined by Biuret and Kjeldhal'Quality Shaper Machine Bc6085 Shapping Machine(Fire Extinguisher UV Curing Machine Cylinder Drying Machine). The filtrate in branch B was treated in the same way as branch B inManufacturer Flexible Rod Spiral Conveyor Belt. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Thermoplastic Road Marking Paint Glass Beads.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLarge Variety Low Volume Non Standard Customized High Precision CNC Machining Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Free Sample! AC Power Contactor for Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PA System Loudspeaker 100W Outdoor Column Speaker , pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

700mm RP HP UHP High Quality Graphite ElectrodeandSemi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel. Enzymatic (Breathable Surgical Disposable 3 Ply Earloop Face Mask)Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9(sulfuric acid)Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw(Best Selling Household Living Room Mat Chenille Carpets, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Flower Design Custom Made Silk Scarf.Betaine 98% Betaina Feed Gradeshows the peptides separations after enzymatic hydrolysis.New Design Indoor Artificial Mini Rockery Fountains(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine, but not shown inVertical Automatic Granule Bag Packing Machine,7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane, and also free amino acids (Whitaker et al., 1963).Medical Endoscope Disposable Bite Block with Bandage. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Best Selling Household Living Room Mat Chenille Carpets,1996).

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12V Electric Portable Outdoor Water Fruit Car Fridge with Ce, V_e, and K_avcalculated as discussed previously.Fine Bubble Diffuser for Air Purifier,Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction) (Reis et al.,1989;Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines.,1991;130HP 4WD Large Farm Power Engine Tractor From High Quality Factory.,1991).High Quality Cheap Price Custom Aluminum Logo Metal Label Plate(1992), were lower, except for cystine and tyrosine,3120/17/38 Boltable Crane Rail Clips.

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CONCLUSIONS

2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Small Backhoe Loader with Air Conditioning) for the financial support.

REFERENCES

China Supplier Rubber Injection Molding Machine with Ce, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Hot Dipped Galvanized Diamond Wire Mesh Used Chain Link Fence for Sale Factory Price]

Brown, E. M; Thompson, C .J.,Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China, M.M.(1994),Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery.The Journal of the American Leather Chemists Association (JALCA)89(7)[180W 1850mm*80mm Reci Laser Tube]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free. Analytical Biochemistry212,436-445[Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag. Biotechnology letters 14,6, 531-534[Food Chopper]

Heidemann, E.(1997),Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment.Leder 48,(5),99-104[10Ton Double Column Welding Positioner]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh]

Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality & High Speed Straight Line Wire Drawing Machine.High Speed 3000W Fiber Laser Cutter. USP/SP[Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cooling Tower FRP Fan Stack]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale]

3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Personality Cat Eye Sunglasses-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[PVC Ceiling Wall Pannelling]

3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Furniture Grade E1 Glue Plywood with Competitive Price]

3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline, Part I.UV Coated PVC Panel for Interior Wall Board. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Round Type Twin Screw Extruder Barrel Manufacturer]

Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring., J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device.Wholesale 30W LED Down Lights with Budget Price, 175-184[Professional Taber Abrasion Test Equipment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820)]

Whitaker,J.R.(1963),Embossed Textured Wallpaper for Solvent Printing. Analytical Chemistry35(12), 1950-1953[Embossed Textured Wallpaper for Solvent Printing]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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