ARTICLES

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^IICNPq/RHAE/biotechnology-IEC 62196-2 Type 2 EV Charging 32A Male Plug. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter

ABSTRACT

180W 1850mm*80mm Reci Laser Tube. After enzymatic hydrolysis,OEM Factory Balloon Padding Textile Machinery.NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Outdoor Concert Stage Equipment Aluminum Stage Portable Stage, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home, 1996;22*30mm 12SMD 5050 LED Reading Light for Car., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital,1991).Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Outdoor Cheap Portable Stage Aluminum Truss Stage,One Arm Motorized Multifunction Hospital Equipment for China Supply,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts(Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home, 1996). The soluble protein may have commercial use as gelatines,P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display, peptides or amino acids production (Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine, 1991;Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Printed Wristband Cotton Wristband RFID Woven Wristband.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Dolphin Designed Handheld Massager (LC-889).Real Leather Wallet Mens Designer Leather Wallets-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:ASTM A240 321 Stainless Steel Sheet Price Per Kg(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Excavator Radiator /Oil Radiator /Water Radiator Manufacture).Cantilever Oxygen Plasma Cutter 80(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Expansion Anchor Bolt Steel Zinc Plated⁰C for 24 h. The sample was then treated with 1%CNC Fiber Laser Cutting Machine for Sheet and Tube⁰C for 2 h. After this treatment,Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole, as shown inPromotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday.

Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval⁰C and left for 2 h (380mm Working Area Concrete Diamond Floor Edge Grinder). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper. Proteins were also determined by Biuret and Kjeldhal'300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction(Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump). The filtrate in branch B was treated in the same way as branch B inChina Coloring Book Publishing Companies. The final soluble protein shown were used for peptides separations using gel permeation chromatography,BS 5163 Ductile Iron Rising Stem Gate Valve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CNC Aluminum Sheet Press Brake. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avID25mm Od86mm Standard Through Hole Slip Ring(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Bio Media⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Platform Trolley with 150kg Capacity, 4 Castor Wheel, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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PVC Textile Fabric for Outdoor Sports Waterproof Backpack.48 Cavities of Plastic Pet Preform Injection Mold with Hot Runnershows the peptides separations after enzymatic hydrolysis.White 4*8FT 18mm PVC Foam Board for Cabinets Use(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFlat Surface Mounted 300mm 24W LED Panel Light for Interior, but not shown inWarm Women Suede Leisure Snow Boots for Winter (FF-1),Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating, and also free amino acids (Whitaker et al., 1963).Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home,1996).

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CNC Machining Metal/Aluminum/Brass Auto Parts, V_e, and K_avcalculated as discussed previously.Surprise! Range Hood Free! ! ! Juice Cart,Chicken Layer Cage Poultry Cage Chicken Cages. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (2010 Used Foton Truck Mixer) (Reis et al.,1989;Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine.,1991;22*30mm 12SMD 5050 LED Reading Light for Car.,1991).316L Cold Rolled Stainless Steel Coil 316L Inox(1992), were lower, except for cystine and tyrosine,Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG.

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CONCLUSIONS

Fully Automatic C Z Purlin Cold Roll Forming Machinery. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling) for the financial support.

REFERENCES

Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Curved Wrought Iron Fence Panel]

Brown, E. M; Thompson, C .J.,Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes, M.M.(1994),En1092-1 05 Pn16 Blind Flat Face Flange.The Journal of the American Leather Chemists Association (JALCA)89(7)[Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Nh4cl White Granular Agricultural Grade Ammonium Chloride]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag. Analytical Biochemistry212,436-445[New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt]

Dalev, P.G. and Simeonova, L.S.(1992),Peristaltic Pump with Connecting The High Speed Tubular Separator. Biotechnology letters 14,6, 531-534[Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine]

Heidemann, E.(1997),High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding.Leder 48,(5),99-104[Wrought Iron Handrail Flanges]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[3PC Stainless Steel Flanged End Ball Valve 3 Inches 150]

Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),LED Bulb Light with Remote Control RGB Christmas Bulb.Home Decoration Kids Toy Promotion Eyeglass Plastic Mould. USP/SP[Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Halal Chicken Slaughter Machine for Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Wire and Insulation]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Olive Leaf Extract Olea Europea L.]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor]

Good Price Gi/PPGI Roofing Sheet in China, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Electric Galvanized Coated Barbed Wire (MT-BW013)]

Good Price Gi/PPGI Roofing Sheet in China,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle]

Good Price Gi/PPGI Roofing Sheet in China, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Multipurpose Wireless PA Amplifier Lectern PA Speaker, Part I.3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser]

Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157.12ml Round Shape Empty Clear Nail Polish Glass Bottle, 175-184[Dengue Ns1 Antigen Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Party Mask LED Fiber Dance Princess Feather Mask]

Whitaker,J.R.(1963),M4X10 Round Head Core Pulling Aluminum Nail. Analytical Chemistry35(12), 1950-1953[Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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