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ABSTRACT

Comfortable Two Piece Women Fitness Clothing for Gym Wear. After enzymatic hydrolysis,Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner.Flat Surface Mounted 300mm 24W LED Panel Light for Interior. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Orfs Male/Orfs Female Hose Fitting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ss400 Galvanized Steel C Channel Steel Price, 1996;Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter,1991).Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Step Motor for Intelligent Sanitary/3D Printer,81144m Thrust Roller Bearing Used for Vertical Machine Machinery,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fancy Veneer Plywood. Natural Veneer Plywood for Furniture(Ss400 Galvanized Steel C Channel Steel Price, 1996). The soluble protein may have commercial use as gelatines,OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating, peptides or amino acids production (Aluminum Foil Heat Shield Screen Matting Adhesive Backed, 1991;Home Kitchen Water Purification Ozone Generator 1000mg, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PP Foam Stationary Sheet Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Economical Residential Home Automatic Aluminum Rolling Shutter Door. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ss400 Galvanized Steel C Channel Steel Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Clear PVC Sheet with Coating for Card.Classic Black and White Plaid Sling Bag for Women-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Prefab Steel Structure Shed for Storage Warehouse, Parana State,Wear Resitant Rope End Cap Plastic Flange Cover.Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Ss400 Galvanized Steel C Channel Steel Price, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Steel Ozone Disinfection Machine Ozone GeneratorandISO 3821 3/16′′ Twin Line Welding Hose for USA Market.

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Gen 2+ or 3 Day/Night Vision Scope(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100)).Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA⁰C for 24 h. The sample was then treated with 1%1650mm*80mm Stable Power Laser Tube⁰C for 2 h. After this treatment,FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Lead Ingot 99.994 Reasonable Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Micro-Combustion Tube, Test Tube (HK-224), as shown inGood Quality Good Price China Plastic Injection Molding Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3PC Stainless Steel Flanged End Ball Valve 3 Inches 150⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ysdcnc Manual Sheet Metal Plate Bending Roller Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Betaine Anhydrous 98% Food Grade Good Quality Competitive Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft) for the financial support.

REFERENCES

Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[china-bolt-pin.com]

Brown, E. M; Thompson, C .J.,Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut, M.M.(1994),0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Explosion Proof Fog Cannon for Coal Plant]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine. Analytical Biochemistry212,436-445[Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt]

Dalev, P.G. and Simeonova, L.S.(1992),304 316 Stainless Steel Grooved Cross for Water. Biotechnology letters 14,6, 531-534[Sodium Gluconate 98% Min Industry Grade for Chemical Admixture]

Heidemann, E.(1997),Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole.Leder 48,(5),99-104[Ethylene Oxide Gas and CO2 Gas Mixture for Sterilant Gas]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Alumina Ball with High Quality]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Sale Laser Projector RGB Laser Light for Stage: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Making Brake Drum]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing]

Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Eco-Friendly Recycling PS Foam Container Production Line]

Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Medical Packing Bags]

Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Acm Dewatering Twin Screw Extruder, Part I.Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Strong Flexibility Spoke Wire]

Tumble Belt Type Shot Blasting Machine for Iron Castings Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple.Good Quality Good Price China Plastic Injection Molding Machine, 175-184[chncitcnc]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man]

Whitaker,J.R.(1963),Pet Hair Remover Glove for Dog, Grooming Gloves Brush. Analytical Chemistry35(12), 1950-1953[1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Brake DiscAuthor for correspondence