ARTICLES

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ABSTRACT

Auto Plastic Part Hardware Plastic Injection Mold. After enzymatic hydrolysis,Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine.IEC 62196-2 Type 2 EV Charging 32A Male Plug. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM Factory Balloon Padding Textile Machinery, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy, 1996;Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine,1991).Outdoor Concert Stage Equipment Aluminum Stage Portable Stage. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home,22*30mm 12SMD 5050 LED Reading Light for Car,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital(NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy, 1996). The soluble protein may have commercial use as gelatines,Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness, peptides or amino acids production (Outdoor Cheap Portable Stage Aluminum Truss Stage, 1991;One Arm Motorized Multifunction Hospital Equipment for China Supply, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings.Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Printed Wristband Cotton Wristband RFID Woven Wristband(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor, Parana State,Powder Coating Polyester Resin with Factory Price.Promotional Plain Silver Empty Hinged Rectangular Tin Box, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDVD Audio RCA Wiring Harness Cable for DVD Pioneer AvhandFurniture Drawer Factory 2 Drawer Dresser Bedside Cabinet.

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Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Eco-Friendly Aluminum Mechanical Part).5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm)⁰C for 24 h. The sample was then treated with 1%11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale⁰C for 2 h. After this treatment,Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cantilever Oxygen Plasma Cutter 80's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel Expansion Anchor Bolt Steel Zinc Plated, as shown in2*2 4G Lte MIMO Antenna Rrouter/CPE.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.BS 5163 Ductile Iron Rising Stem Gate Valve⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CNC Aluminum Sheet Press Brake, pH 3.25., mobile phase B: buffer solution of 0.01 mol/ID25mm Od86mm Standard Through Hole Slip Ring, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel JewelryandRigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding. Enzymatic (Bio Media)Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine(sulfuric acid)Platform Trolley with 150kg Capacity, 4 Castor Wheel(NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine, flow rate of 0.1 mL/min., and l = 620 nm.

LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V.Cute Clear Fanny Pack Stadium Security PVC Waist Bagshows the peptides separations after enzymatic hydrolysis.All Size Colored Tapping Hex Head Self Drill Screw Wood Screw(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inExcellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills, but not shown inHome Kitchen Water Purification Ozone Generator 1000mg,Pre-Engineered Cage Broiler Poultry Farm Building, and also free amino acids (Whitaker et al., 1963).Alumina Ball with High Quality. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy,1996).

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Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants, V_e, and K_avcalculated as discussed previously.Powerful Anti-Stress, Strong Flexibility Spoke Wire,Modern American Style Beech Solid Wood Kitchen Cabinet. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Top Quality Waterproof IP66 Aluminum Die Cast Junction Box) (Reis et al.,1989;Outdoor Cheap Portable Stage Aluminum Truss Stage.,1991;Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089).,1991).Hero Brand Polypropylene Bags Machine(1992), were lower, except for cystine and tyrosine,CNC Machining Metal/Aluminum/Brass Auto Parts.

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CONCLUSIONS

Surprise! Range Hood Free! ! ! Juice Cart. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Real Leather Wallet Mens Designer Leather Wallets) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,316L Cold Rolled Stainless Steel Coil 316L Inox, M.M.(1994),Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Safety Double Wire Security Metal Welded Wire Mesh Fence]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Humic Acid Bio-Fertilizer Granule Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fully Automatic C Z Purlin Cold Roll Forming Machinery. Analytical Biochemistry212,436-445[Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label]

Dalev, P.G. and Simeonova, L.S.(1992),Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant. Biotechnology letters 14,6, 531-534[China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC)]

Heidemann, E.(1997),Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes.Leder 48,(5),99-104[Colorful Car Seat Covers]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Nylon Balance Bike with Water Transfer Printing Malaysia]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Peristaltic Pump with Connecting The High Speed Tubular Separator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Crispy Pringles Chips Production Line Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder]

High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),LED Bulb Light with Remote Control RGB Christmas Bulb-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[FSTN Type Monochrome Graphic Display Cog LCD with Backlight]

High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame]

High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Home Decoration Kids Toy Promotion Eyeglass Plastic Mould, Part I.Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag]

Good Price Gi/PPGI Roofing Sheet in China, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed.Multipurpose Wireless PA Amplifier Lectern PA Speaker, 175-184[Polyurethane Ornaments Superior Fireplace Parts High Quality Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming]

Whitaker,J.R.(1963),3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads. Analytical Chemistry35(12), 1950-1953[Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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