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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Headband Big Headphone EVA Bag Tool Case Specialized Storage Box, 1996;Chicken Slaughtering Machine for Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish,1991).Hf Vacuum Dryer Machine with Advanced High Frequency Technology. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers,Sunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai(Headband Big Headphone EVA Bag Tool Case Specialized Storage Box, 1996). The soluble protein may have commercial use as gelatines,2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women, peptides or amino acids production (Tonva New Design Harpic Bottle Making Extrusion Blow/Blowing Molding Machine, 1991;Oak Laminate Grey Floating Cork Vinyl Plank Flooring, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Manufacturer Customized Microwave Ring Ferrite Magnet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Competitive Price Good Quality Custom Unisex Marle Grey Fleece Hoody. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Headband Big Headphone EVA Bag Tool Case Specialized Storage Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Printing Adhesive Waterproof Custom Membrane Switch for Electric Cooker.Electrical 4W AC Synchronous Gear Motor for Engraving Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Car Seat Belt Buckle Mold Maker(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av48V DC Powered Air Conditioner for Outdoor Cabinet 2000W(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sleeved Wire Harness for Industry Equipment⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Home Use Standing Modern Floor Lamp (KAF6108), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Real Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for Watch, Jewelry) for the financial support.

REFERENCES

Automatic Three Side Sealing Facial Mask Bag Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Speed Rg59 2c Power Coaxial Wholesale Rg59 Video Power Cable Best Price CCTV Cable]

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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant. Analytical Biochemistry212,436-445[for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Wireless Cheapest Android 2D Barcode Scanner]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High-Quality Pre-Seasoned Cast Iron Wok]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-5W Ultra-Thin Tiltable LED Down Light: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Parts For Massage Chairs]

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Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Good Quality NBR70 O-Ring with Reasonable Prices, Part I.Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Products Genuine Leather En345-1 Steel Toe Cap Leather Safety Boots]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[U. S. Type Drop Forged Wire Rope Clip]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019)Author for correspondence