ARTICLES

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ABSTRACT

4 Drawer Steel Office Vertical Filing Cabinet. After enzymatic hydrolysis,12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car.Parts for Crane Outrigger Hydraulic Oil Cylinder. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Boned Polyester Woven Faux Suede Sofa Fabric;Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.20 Bottles Electric Wine Display Cooler, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High-Efficient Htk Walnut Shell Filter for Water Purification, 1996;Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100),1991).Popular European Style White Marble Coffee Tables for Dinnig Room. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket,FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Kwikstage Scaffolding System-Diagonal Brace(High-Efficient Htk Walnut Shell Filter for Water Purification, 1996). The soluble protein may have commercial use as gelatines,360 Rotating Automatic Touchless Car Wash Equipment with Drying System, peptides or amino acids production (48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner, 1991;Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Sell Rubber / EVA / PE/PVC Foam Board.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sodium Gluconate 98% Min Industry Grade for Chemical Admixture. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High-Efficient Htk Walnut Shell Filter for Water Purification, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Brake Disc.DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

360 Degree Rotation Medical Seat Cushion, Parana State,Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin.Custom LCD Screen Video Box for Package, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High-Efficient Htk Walnut Shell Filter for Water Purification, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theZipper Pouch Pistachio Nuts Premade Packing MachineandPersonality Cat Eye Sunglasses.

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Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Custom Self Adhesive Leather Patch for Jackets).Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine⁰C for 24 h. The sample was then treated with 1%Alu 2319 Slimline 12mm Wide Recessed Profile⁰C for 2 h. After this treatment,High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom Size Christmas Gift Personalized Watch Boxes for Men's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope, as shown inOutsole Trimming Machine.

8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector⁰C and left for 2 h (Airtight Candy Bin with Scoop). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115. Proteins were also determined by Biuret and Kjeldhal'China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX)(Auto Spare Parts Ignition Coil Fp85-18-100c). The filtrate in branch B was treated in the same way as branch B in70ml Plastic Bottle. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Cross Recesed Flat Head Self Tapping Screw.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Our Perforated Aluminum & Solid Panel for New Office Wall Cladding. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wd190V Handheld Full Body Scanner Hand Held Metal Detector⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hydraulic Orbital Valve Power Steering, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game MachineandW11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine. Enzymatic (AISI 410 Stainless Steel Galvanized Wire for Making Scourer)CS1363 a Standard UK 3 Pin Power Plug(sulfuric acid)Food Chopper(High-Efficient Htk Walnut Shell Filter for Water Purification, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile, flow rate of 0.1 mL/min., and l = 620 nm.

Wholesale Prices of 20W 40W 60W Solar Street Lights.Side Pack Manual Gas Engine Power Brush Cuttershows the peptides separations after enzymatic hydrolysis.ISO 3821 3/16′′ Twin Line Welding Hose for USA Market(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStationery PP Clipboard With 7 Inch Scale Ruler, but not shown inFarm Machine Parts Spring Harrow Tines/ Fork Tedder Tines,Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins, and also free amino acids (Whitaker et al., 1963).Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High-Efficient Htk Walnut Shell Filter for Water Purification,1996).

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CONCLUSIONS

Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (LED Screen Display Wireless Charging 10000mAh Power Bank) for the financial support.

REFERENCES

UCP Color Army Camouflage Uniform Army Military Uniform Suit, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Camshaft Position Sensor]

Brown, E. M; Thompson, C .J.,0.5 Gallons Cool Mist Ultrasonic Humidifier, M.M.(1994),Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sanjing PVC Lining Layflat Fire Hose. Analytical Biochemistry212,436-445[Hemp Seed Oil Body Lotion Moisturizes The Skin]

Dalev, P.G. and Simeonova, L.S.(1992),Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar. Biotechnology letters 14,6, 531-534[10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor]

Heidemann, E.(1997),China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light.Leder 48,(5),99-104[Hot Sale Bathroom and Kitchen Ceramic Wall Tile Buiding Material (300*600 mm)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Kitchen Metal Dish Storage Rack (JP-SC984C)]

Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Automatic CNC Glass Cutting Machine Glass Cutting Table.Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory. USP/SP[ Aerospace Equipment]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[BS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hip Hop Jewelry Pendants]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition]

Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pet PP Rope and Broom Wire Drawing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ LED Bayomet Vehicle Lamps]

Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Phillip Countersunk Head Screw Hot DIP Galvanized]

Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup, Part I.PTFE Seat Wafer Butterfly Valve Pn16. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic CNC Glass Cutting Machine Glass Cutting Table]

Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults.Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China, 175-184[Front Glass Panels]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook]

Whitaker,J.R.(1963),Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01). Analytical Chemistry35(12), 1950-1953[Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar LightAuthor for correspondence