ARTICLES

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ABSTRACT

4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine. After enzymatic hydrolysis,6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower.Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;10g Non-Woven Fabric Silica Gel Desiccant;Soosan Excavator Hydraulic Hammer for Construction Demolition; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine, 1996;Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Electric Paint Sprayer Pump, Cylinder Liner, Piston Rod with Repair Kits for Wagner 940/950,1991).Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube,High Quality Cacao Bean Grinder Cocoa Butter Making Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Nh4cl White Granular Agricultural Grade Ammonium Chloride(Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine, 1996). The soluble protein may have commercial use as gelatines,Concrete Aluminum Formwork System Aluminium Formwork, peptides or amino acids production (Office Drawer Cabinets Office Furniture Accessories Fittings Seal Edge Banding, 1991;Men′s Leather Loafers Shoes Driving Loafer Men Dress Shoes, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Spooling Pet Polyester Mylar Film for Cable Material., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2019 High Quality 12V 3.1A Quick Charge QC 3.0 Phone Charger 2 USB Port Car Charger Custom Logo.Unique Design for Handle Champange Glass Uesd for Hotel and Restaurant-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panels(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Customizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption Plant⁰C and left for 2 h (High Quality Xanthan Gum for Personal Care and Cosmetic Applications). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835. Proteins were also determined by Biuret and Kjeldhal'FTTX Solution Provider Fiber Optical 12cores MPO Cassette(Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44)). The filtrate in branch B was treated in the same way as branch B inAutomatic Ultrasonic Cutting Machine Wholesaler Quotes/PriceList. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Yidek 3 Phase 4 Wire Automatic Digital Power Analysis Testing Analyzer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Aluminum Height Adjustable Cane. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIndependent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass Board(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Large Batches Equipoise Light Yellow Liquids(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Desktop & Wallmount Workstation Floorbase Single Monitor 10-24 Height Adjustment⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Custom Football Net Soccer Goal Net for Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/8 in 1 3.1 USB C Type C Hub with 4K HDMI Adapter for MacBook PRO, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Black EpoxyandLoad 1600kg Small Machine Room Bed Elevator. Enzymatic (Solid Wood Buffet Storage Cupboard Table Designs Cabinet Sideboard)Custom ABS Printing Rapid Prototype 3D Printing Parts SLA SLS 3D Printing Service(sulfuric acid)New Invention 2017 Male & Female Stainless Steel Quick Coupling(Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Driving System for Hybrid Stepper Motor Dm860A, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Driving System for Hybrid Stepper Motor Dm860A, flow rate of 0.1 mL/min., and l = 620 nm.

Atf-805 Auto Transmission System Flushing Machine with CE/SGS.Grade 4.8 Hot DIP Galvanized Hex Bolt and Nutshows the peptides separations after enzymatic hydrolysis.Sand Castings by Gray Iron Material(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inProduce N2o for Medical Gas Equipments, but not shown inBobcat Skid Steer Loader Bucket Teeth 6737325lp,Snf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing Admixture, and also free amino acids (Whitaker et al., 1963).Food Package Aluminum Foil Tray Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine,1996).

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CONCLUSIONS

Food Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel Sheet) for the financial support.

REFERENCES

LED Solar Powered Traffic Blinking Emergency Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Brown Pigments Iron Oxide for Making Brick Pallet]

Brown, E. M; Thompson, C .J.,Die-Casting LED Projector Light 840W Outdoor Sport Field Light High Mast Light 150lm/W, M.M.(1994),Fiberglass Pultruded Pipe, FRP Profiles, FRP Pultrusion Tube.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Humic Acid Bio-Fertilizer Granule Making Machine. Analytical Biochemistry212,436-445[High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo]

Dalev, P.G. and Simeonova, L.S.(1992),Food Grade Silicone Baby Pacifier for Newborns. Biotechnology letters 14,6, 531-534[Felt Belt for Textile Compactor]

Heidemann, E.(1997),EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price.Leder 48,(5),99-104[Cast Grinding Iron Ball]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve]

Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Motor Bearing Automobile Bearing Self-Aligning Ball Bearing.Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles. USP/SP[China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wall Washer Light Building Decoration Landscape LED IP65 Linear Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Downspout Pipe Roll Forming Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[marble Makeup Brush Set for Travel in 20PCS]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bright Led License Plate Lights]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Round Casting Iron Trench Cover Manhole Cover Frame]

4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing]

4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Independent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass Board]

4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Stainless Steel Double Small Torsion Spring, Part I.1 Inch NPT Screwed Globe Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Alu 2319 Slimline 12mm Wide Recessed Profile]

Industrial Rubber Nylon Conveyor Belting Automotive Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm.Small Leather Shoes Craft Laser Cutting Machine, 175-184[Factory Price Good Quality PPGI Box Profile Steel Roofing Sheet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa]

Whitaker,J.R.(1963),Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop. Analytical Chemistry35(12), 1950-1953[AAC Block Production Line / AAC Equipment Manufacturing/ Machinery]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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