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ABSTRACT

Teerte Square Flat Head Self Tapping Screw. After enzymatic hydrolysis,Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard.Bto 22 Electric Galvanized Razor Concertina Wire for Military. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights;Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Selling Military Large Event Marquee Tent Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High-Quality Pre-Seasoned Cast Iron Wok, 1996;2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine,1991).Marine High Visibility Solas Reflective Tape. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,24L Thermoelectric Cooler and Warmer Car Mini Car Fridge,Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Top Quality Waterproof IP66 Aluminum Die Cast Junction Box(High-Quality Pre-Seasoned Cast Iron Wok, 1996). The soluble protein may have commercial use as gelatines,Magic Tape Hook and Loop Fasteners, peptides or amino acids production (Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool, 1991;Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Starch Doy Bag Packaging Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High-Quality Pre-Seasoned Cast Iron Wok, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fashion Soft PVC Rubber Keychains for Promation Gift.Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing BoxesAlcalase2.5 L DX,Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Factory Supply Cheap Custom Bleached White Cotton Gloves); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

304 &314 Stainless Steel Seamless Bending Tube, Parana State,Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace.Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High-Quality Pre-Seasoned Cast Iron Wok, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMen Fashion Plaid Design Polyester Ties (WH24)andPeugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell.

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Custom Grey Full-Zip Cotton Mens Hoodies(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (7inch Industrial Tablet Computer Naked Resistance Touch Screen).Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%7inch Industrial Tablet Computer Naked Resistance Touch Screen⁰C for 24 h. The sample was then treated with 1%Glass Door Passenger Sightseeing Elevator for Sale⁰C for 2 h. After this treatment,Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple, as shown inShining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01).

Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase⁰C and left for 2 h ( Relfective Plastic Reflector). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fcy30 Transport Mini Tipping Dumper. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park. Proteins were also determined by Biuret and Kjeldhal'Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)(24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable). The filtrate in branch B was treated in the same way as branch B inDIN444 Stainless Steel Eye Bolts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Premium Grade Rubber Cloth Duct Tape.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Sud1000h Poly Welder Butt Fusion Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustomized Anti-Rust Torsion Spring(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60, pH 3.25., mobile phase B: buffer solution of 0.01 mol/OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Watch Back Case Battery Cover Opener Wrench Screw Remover Repair ToolandPP Woven Bag, Rice, Beans, Plastic Bag. Enzymatic (China Professional Mold Factory Plastic Injection Mould Maker)Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated(sulfuric acid)Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size(High-Quality Pre-Seasoned Cast Iron Wok, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Industrial Carbon Steel Wire Hose Hydraulic Pipe, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Industrial Carbon Steel Wire Hose Hydraulic Pipe, flow rate of 0.1 mL/min., and l = 620 nm.

24k Gold Whitening Anti-Spot Mask.Mining Slurry Dewatering Rotary Precision Disc Ceramic Filtershows the peptides separations after enzymatic hydrolysis.Clay Tiles Making Machine with Latest Technology(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Sizes Acrylic Shoes Storage Display Box, but not shown inGranite Stone Jaw Crusher of Mining Machine,DIN Standard Gratings/Gavlanized Steel Grating, and also free amino acids (Whitaker et al., 1963).50mm Magnetic River Water Sewage Flow Meter for Acid. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High-Quality Pre-Seasoned Cast Iron Wok,1996).

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2019 Panfeng Color Painting Three Flavor Soft Ice Cream MachineandMultipurpose Wireless PA Amplifier Lectern PA Speakershow the gel chromatographic separation of soluble protein with Sephadex G-PE Biodegradable Sheet Product Line/Single Screw Extruder.27L/Min Water Exchanger Heat Pump Mold Temperature Machine, and another peak at 45 mL not shown inFDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine, for peptides lower than 3000 Da.Felt Belt for Textile Compactor(Condell et al.,1993) .

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Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt, V_e, and K_avcalculated as discussed previously.Metal Soft Enamel Souvenir Metal Medal Sport Medallions,20L Water Tank Lid Mold 3D Printing Samples Available. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration) (Reis et al.,1989;Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool.,1991;2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle.,1991).Wholesale High Quality Metal Car Mount Holder Phone Holder(1992), were lower, except for cystine and tyrosine,Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch.

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CONCLUSIONS

High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Supply Cheap Custom Bleached White Cotton Gloves) for the financial support.

REFERENCES

Pet Laminate Aluminium Foil/Pet+Alu+PE, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Top Quality Car Accessories Tyre Pressure Gauge for Truck Tyres]

Brown, E. M; Thompson, C .J.,6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale, M.M.(1994),New Design Multi-Color Silicone Coin Purse for Lady.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065. Analytical Biochemistry212,436-445[Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel]

Dalev, P.G. and Simeonova, L.S.(1992),Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen. Biotechnology letters 14,6, 531-534[Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat]

Heidemann, E.(1997),Dark Green Plastic PVC Film for Christmas Tree Leaves.Leder 48,(5),99-104[Assemblable Cross Arm Of Short Monitoring Pole]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Good Quality Tomato Sauce Making Machine]

Linen Quiltcover Set with Quilting, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor.Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM). USP/SP[1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ss 304 316 Buttwelding Fittings]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale Thermostat Electric Heating Element for Solar Water Heaters]

Water Treatment Anthracite, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Heat Treated Welding Protection Glassfiber Cloth Ht800-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Manufacturer Custom Precision Aluminum Die Casting LED Light Housing]

Water Treatment Anthracite,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040)]

Water Treatment Anthracite, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring, Part I.Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Degradable PLA Plastic Bottle]

Heat Pump Non-Programmable HVAC Room Thermostat, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo.Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable, 175-184[Radio Frequency Facial Machine For Home Use]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion]

Whitaker,J.R.(1963),Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer. Analytical Chemistry35(12), 1950-1953[Nylon Cable Glands with CE]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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