ARTICLES

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ABSTRACT

Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck. After enzymatic hydrolysis,Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module.ADSS Newest Ultra Therapy Skin Tightening 3D Hifu. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap;Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Ceramic Chrome Hybrid Bearing Oil, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown, 1996;CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Digger Arm Small Wheel Loader (HQ908) with CE,1991).15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output,Water Based Emulsion Paint Production Line,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cnm Single Strand Anchor Coupler(Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown, 1996). The soluble protein may have commercial use as gelatines,Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp, peptides or amino acids production (18X16mesh Fiberglass Window Screen Insect Screen, 1991;Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sterile Dsiposable Syringe for Dental 12ml., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Foldable Bed Military Cot for Camping.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-P5 960*960mm LED Display Screen SMD Indoor /Outdoor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2019 New Advertising Inflatables Air Walking Backpack Ball.Side Pack Manual Gas Engine Power Brush Cutter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Punching Bending Welding Steel Work Fabrication(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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IEC 62196-2 Type 2 EV Charging 32A Male Plug(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine).PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%180W 1850mm*80mm Reci Laser Tube⁰C for 24 h. The sample was then treated with 1%OEM Factory Balloon Padding Textile Machinery⁰C for 2 h. After this treatment,NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine, as shown in3PC Stainless Steel Flanged End Ball Valve 3 Inches 150.

Outdoor Concert Stage Equipment Aluminum Stage Portable Stage⁰C and left for 2 h (Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,22*30mm 12SMD 5050 LED Reading Light for Car. Proteins were also determined by Biuret and Kjeldhal'SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital(Halal Chicken Slaughter Machine for Sale). The filtrate in branch B was treated in the same way as branch B in4mm Epdm Flooring Factory OEM/ODM Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Outdoor Cheap Portable Stage Aluminum Truss Stage. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOne Arm Motorized Multifunction Hospital Equipment for China Supply(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wire and InsulationandMetallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery. Enzymatic (Printed Wristband Cotton Wristband RFID Woven Wristband)High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles(sulfuric acid)Dolphin Designed Handheld Massager (LC-889)(Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Real Leather Wallet Mens Designer Leather Wallets, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Real Leather Wallet Mens Designer Leather Wallets, flow rate of 0.1 mL/min., and l = 620 nm.

ASTM A240 321 Stainless Steel Sheet Price Per Kg.12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motorshows the peptides separations after enzymatic hydrolysis.V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inElectric Galvanized Coated Barbed Wire (MT-BW013), but not shown inHardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle,Promotional Plain Silver Empty Hinged Rectangular Tin Box, and also free amino acids (Whitaker et al., 1963).Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown,1996).

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Party Mask LED Fiber Dance Princess Feather MaskandPipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotatorshow the gel chromatographic separation of soluble protein with Sephadex G-5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer.Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm), and another peak at 45 mL not shown inDuctile Iron Standard Brackets Pipe Hanger, for peptides lower than 3000 Da.11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale(Condell et al.,1993) .

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CONCLUSIONS

Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (1 Ton Chain Pulley Block with Geared Trolley) for the financial support.

REFERENCES

Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Skycom FTTH Tool Kit Optical Fusion Splicer Price]

Brown, E. M; Thompson, C .J.,Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval, M.M.(1994),High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump.The Journal of the American Leather Chemists Association (JALCA)89(7)[Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper. Analytical Biochemistry212,436-445[160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures]

Dalev, P.G. and Simeonova, L.S.(1992),300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction. Biotechnology letters 14,6, 531-534[Line Scan Uvss System Portable Under Vehicle Surveillance]

Heidemann, E.(1997),BS 5163 Ductile Iron Rising Stem Gate Valve.Leder 48,(5),99-104[Hex Head Self Drilling Screws]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank]

Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),CNC Aluminum Sheet Press Brake.ID25mm Od86mm Standard Through Hole Slip Ring. USP/SP[Electric 100W Three Wheels Toy Car for Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1 3 5 10 20 50 100 Micron High Flow Pleated Cartridges Water Filter for Purification]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication]

Bio Media, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box]

Bio Media,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Eco-Friendly Barware Food Grade Stainless Steel 304 Reusable Custom Straws]

Bio Media, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Platform Trolley with 150kg Capacity, 4 Castor Wheel, Part I.Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Heat Treated Welding Protection Glassfiber Cloth Ht800]

LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),All Size Colored Tapping Hex Head Self Drill Screw Wood Screw.Pre-Engineered Cage Broiler Poultry Farm Building, 175-184[Perfume Polymer Clay Gasbag Fragrance Bottle Spray Atomizer Glass Essential Oil Bottle with Hanging Diffuser Cap]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Condition Hot Sale Automatic Animal Feed Cooler Machine]

Whitaker,J.R.(1963),Alumina Ball with High Quality. Analytical Chemistry35(12), 1950-1953[Bio Media]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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