ARTICLES

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ABSTRACT

Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine. After enzymatic hydrolysis,Hot Selling Military Large Event Marquee Tent Sale.High-Quality Pre-Seasoned Cast Iron Wok. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Marine High Visibility Solas Reflective Tape, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (24L Thermoelectric Cooler and Warmer Car Mini Car Fridge, 1996;Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Top Quality Waterproof IP66 Aluminum Die Cast Junction Box,1991).Magic Tape Hook and Loop Fasteners. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool,Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor(24L Thermoelectric Cooler and Warmer Car Mini Car Fridge, 1996). The soluble protein may have commercial use as gelatines,Starch Doy Bag Packaging Machine, peptides or amino acids production (High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A), 1991;Fashion Soft PVC Rubber Keychains for Promation Gift, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (24L Thermoelectric Cooler and Warmer Car Mini Car Fridge, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments.Factory Supply Cheap Custom Bleached White Cotton Gloves-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

7inch Industrial Tablet Computer Naked Resistance Touch Screen, Parana State,Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights.Glass Door Passenger Sightseeing Elevator for Sale, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (24L Thermoelectric Cooler and Warmer Car Mini Car Fridge, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBest Polishing Pads Concrete Grinding Discs Diamond Sanding Discsand330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing.

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Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Medical Packing Bags).12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple⁰C for 24 h. The sample was then treated with 1%Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase⁰C for 2 h. After this treatment,Fcy30 Transport Mini Tipping Dumper. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250), as shown in Strong Flexibility Spoke Wire.

Premium Grade Rubber Cloth Duct Tape⁰C and left for 2 h (165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Sud1000h Poly Welder Butt Fusion Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Customized Anti-Rust Torsion Spring. Proteins were also determined by Biuret and Kjeldhal'Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining(1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine). The filtrate in branch B was treated in the same way as branch B in1-1/4 Hemp Rolling Paper/Natrual Arabic Gun Unbleached Smoking Rolling Paper. The final soluble protein shown were used for peptides separations using gel permeation chromatography,New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Professional Mold Factory Plastic Injection Mould Maker⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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PE Biodegradable Sheet Product Line/Single Screw Extruder.Jm305 Hot Sell Single Bowl Kitchen Sink with Small Sizeshows the peptides separations after enzymatic hydrolysis.27L/Min Water Exchanger Heat Pump Mold Temperature Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Customized Aluminum Foil Shisha Sheet, but not shown inHigh Quality Mini LED Flood Light SMD Flood Light 50W,Felt Belt for Textile Compactor, and also free amino acids (Whitaker et al., 1963).Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (24L Thermoelectric Cooler and Warmer Car Mini Car Fridge,1996).

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CONCLUSIONS

Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Grey Full-Zip Cotton Mens Hoodies) for the financial support.

REFERENCES

Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Concrete Pan Mixer for Sale Manufacturers From China Tobemac Brand]

Brown, E. M; Thompson, C .J.,Dark Green Plastic PVC Film for Christmas Tree Leaves, M.M.(1994),Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor.The Journal of the American Leather Chemists Association (JALCA)89(7)[Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM). Analytical Biochemistry212,436-445[Steel Bar Peeling Lathe Machine]

Dalev, P.G. and Simeonova, L.S.(1992),1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction. Biotechnology letters 14,6, 531-534[Pipe Sheet Laser Cutter with Tube Cutting]

Heidemann, E.(1997),Water Treatment Anthracite.Leder 48,(5),99-104[High Performance 10 Molding Channels Round Tube Change Square Tube Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Galfan Wire (Aluminium-Zinc Alloy coated)]

Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Heat Treated Welding Protection Glassfiber Cloth Ht800.Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring. USP/SP[LED Screen Display Wireless Charging 10000mAh Power Bank]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pearls Flake 99% Factory Naoh Caustic Soda]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[mypestkiller]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640)]

Heat Pump Non-Programmable HVAC Room Thermostat, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681)]

Heat Pump Non-Programmable HVAC Room Thermostat,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Parts for Crane Outrigger Hydraulic Oil Cylinder]

Heat Pump Non-Programmable HVAC Room Thermostat, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable, Part I.Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine]

Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Injector Pump Calibration Test Bench.ASME/ASTM Butt-Welding Stainless Steel Plate Flange, 175-184[7800 series]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom Car Seat Belt Buckle Mold Maker]

Whitaker,J.R.(1963),500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank. Analytical Chemistry35(12), 1950-1953[Baby Feeding Chair Plastic Chair]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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