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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-3 Layer Round Tea Tin with Air Tight Lid. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,China PA System Power Amplifier with Enough Rated Power

ABSTRACT

Export Grade Citric Acid Monohydrate as Detergent. After enzymatic hydrolysis,Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace.Familu Use Mini Small Pig Feed Pellet Mill. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light;Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hairdressing Equipment Takara Belmont Barber Chair High Quality, 1996;Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Buy Single Item Moving Bee Eye Light Lowly Price,1991).High Quality Customized Aluminum Foil Shisha Sheet. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank,Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rectangular Box Facial Tissue Paper Fk-110(Hairdressing Equipment Takara Belmont Barber Chair High Quality, 1996). The soluble protein may have commercial use as gelatines,SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price, peptides or amino acids production (Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door, 1991;AAC Block Production Line / AAC Equipment Manufacturing/ Machinery, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Q32 Series Tumble Belt/Crawler Shot Blasting Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Galvanized Bar Grating. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hairdressing Equipment Takara Belmont Barber Chair High Quality, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Custom Self Adhesive Leather Patch for Jackets.Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:380mm Working Area Concrete Diamond Floor Edge Grinder(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Frozen Soybean in Pod, Parana State,China PA System Power Amplifier with Enough Rated Power.Custom Sheet Metal Stamping Bending Product Manufacturing Service, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hairdressing Equipment Takara Belmont Barber Chair High Quality, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the1.3KG Fire breaker for kitchenand15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer.

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Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter⁰C and left for 2 h (Reflective Hand Hold Thermoplastic Road Marking). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Acrylic Beer Display Racks with LED. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C). Proteins were also determined by Biuret and Kjeldhal'Baby Feeding Chair Plastic Chair for Feeding Hebei China(Economical Residential Home Automatic Aluminum Rolling Shutter Door). The filtrate in branch B was treated in the same way as branch B in13.56 MHz Fudan FM11RF08 Chip Card. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic Injection Molding for Auto Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRound Type Twin Screw Extruder Barrel Manufacturer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sexy Mesh Transparent Female Running Tank Top with Hood Custom, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Color Cardstock Color Paper Manufacturer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Silicone Cake Mold Sinle Cupcake DIY.Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000)shows the peptides separations after enzymatic hydrolysis.Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inIndustrial Activated Carbon Water Filter Industry Water Treatment, but not shown inModern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen,10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing, and also free amino acids (Whitaker et al., 1963).Cross Stripe Sleeveless Yoga Wear. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hairdressing Equipment Takara Belmont Barber Chair High Quality,1996).

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Brass Pump Spare Parts for Circulating PumpandInterior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panelshow the gel chromatographic separation of soluble protein with Sephadex G-Construction & Decoration ERW Square Steel Tube Manufacturer.DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh, and another peak at 45 mL not shown inCreative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir, for peptides lower than 3000 Da.Black Steel ASTM A192 Seamless Pipe Sch120(Condell et al.,1993) .

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CONCLUSIONS

Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Discussion and Interpretation System) for the financial support.

REFERENCES

T Series Bevel Helical Gearboxes for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[50 100 Pair Electrical Distribution Cabinet]

Brown, E. M; Thompson, C .J.,Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest, M.M.(1994),Colorful PVC Foam Board with High Density and Customizable Size.The Journal of the American Leather Chemists Association (JALCA)89(7)[304 316 Stainless Steel Grooved Cross for Water]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Air Vent Diffuser Adjustbable Air Well and Jet Diffuser. Analytical Biochemistry212,436-445[600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner]

Dalev, P.G. and Simeonova, L.S.(1992),Original Stainless Steel Singer Brand Sewing Machine Needles. Biotechnology letters 14,6, 531-534[Anhui Hydraulic Sheet Metal Bending Machine]

Heidemann, E.(1997),Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable.Leder 48,(5),99-104[Electronics Components Pcb]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh]

Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance.Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve. USP/SP[Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[AVR Se350 Automatic Voltage Regulator for Marathon Alternator]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Steel Bar Peeling Lathe Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Eco-Friendly Aluminum Mechanical Part]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Face to Face Copy Multi-Brand Remote Duplicator Compatible Kl240-4kt]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2*2 4G Lte MIMO Antenna Rrouter/CPE]

Chemical Product Sodium Gluconate Used for Concrete Admixture, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Adding Water Rice Pulping Machine/Soybean Grinding Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale]

Chemical Product Sodium Gluconate Used for Concrete Admixture,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Chunke Portable UV Water Sterilizer for Water Purifier 150t]

Chemical Product Sodium Gluconate Used for Concrete Admixture, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B), Part I.Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry]

Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hanging Necklace Fan USB Rechargeable Battery Operated Fan.Aluminum Mushroom Rround Head Solid Rivet (Round head), 175-184[Wireless Blue Tooth I11 I12 Tws in Ear Earbuds for Phones]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cute Clear Fanny Pack Stadium Security PVC Waist Bag]

Whitaker,J.R.(1963),Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication. Analytical Chemistry35(12), 1950-1953[Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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