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ABSTRACT

Phillip Countersunk Head Screw Hot DIP Galvanized. After enzymatic hydrolysis,Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse.Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Heat Shrink Automatic Flow Wrapping Packing Machine;Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ductile Iron Standard Brackets Pipe Hanger, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers, 1996;ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building,1991).High Quality Sheet Metal Bending Roll Forming Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Different Size Glass Test Tubes, High Quality Laboratory Test Tubes,Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Frame Type Rubber Hydraulic Press Machine(Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers, 1996). The soluble protein may have commercial use as gelatines,Kitchen Metal Dish Storage Rack (JP-SC984C), peptides or amino acids production (0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming, 1991;AVR Se350 Automatic Voltage Regulator for Marathon Alternator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Strength Rigid PVC Plastic Foam Board for FRP. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building.4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (U. S. Type Drop Forged Wire Rope Clip).Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Ceramic Chrome Hybrid Bearing Oil⁰C for 24 h. The sample was then treated with 1%Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown⁰C for 2 h. After this treatment,CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Digger Arm Small Wheel Loader (HQ908) with CE's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer, as shown inFashion Men′s Clothing Shop Interior Design Clothes Store Fixtures.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSterile Dsiposable Syringe for Dental 12ml(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.P5 960*960mm LED Display Screen SMD Indoor /Outdoor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2019 New Advertising Inflatables Air Walking Backpack Ball, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Side Pack Manual Gas Engine Power Brush Cutter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Auto Plastic Part Hardware Plastic Injection Mold.12ml Round Shape Empty Clear Nail Polish Glass Bottleshows the peptides separations after enzymatic hydrolysis.Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDiamond Segments 2000mm Diameter Granite Cutting Blade, but not shown in2019 New Advertising Inflatables Air Walking Backpack Ball,IEC 62196-2 Type 2 EV Charging 32A Male Plug, and also free amino acids (Whitaker et al., 1963).PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers,1996).

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CONCLUSIONS

SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels) for the financial support.

REFERENCES

Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Baby Fur Coat Wholesaler Quotes & PriceList]

Brown, E. M; Thompson, C .J.,Outdoor Cheap Portable Stage Aluminum Truss Stage, M.M.(1994),One Arm Motorized Multifunction Hospital Equipment for China Supply.The Journal of the American Leather Chemists Association (JALCA)89(7)[Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Korean Integrated CTO Activated Carbon Filter for Water Treatment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts. Analytical Biochemistry212,436-445[Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using]

Dalev, P.G. and Simeonova, L.S.(1992),P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display. Biotechnology letters 14,6, 531-534[0.5 Gallons Cool Mist Ultrasonic Humidifier]

Heidemann, E.(1997),Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine.Leder 48,(5),99-104[Fried Onion Powder]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price]

10mm Red Green Yellow Warning China Supplier Signal Lamp, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings.Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery. USP/SP[Promotional Customized PP Hand Fan]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Auto Plastic Part Hardware Plastic Injection Mold]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Printed Wristband Cotton Wristband RFID Woven Wristband: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminum Profiles For Glass]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Chemical Plant Transfer Industrial Water Pump]

High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dolphin Designed Handheld Massager (LC-889)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital]

High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector]

High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Real Leather Wallet Mens Designer Leather Wallets, Part I.ASTM A240 321 Stainless Steel Sheet Price Per Kg. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[60m3/H Lamella Clarifier for River Water Treatment]

V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Promotional Plain Silver Empty Hinged Rectangular Tin Box.Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling, 175-184[Liên Hệ]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut]

Whitaker,J.R.(1963),5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer. Analytical Chemistry35(12), 1950-1953[Starch Doy Bag Packaging Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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