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ABSTRACT

Professional Manufacture Commercial Use Spice Grinder. After enzymatic hydrolysis,Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant.Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker;Wall Washer Light Building Decoration Landscape LED IP65 Linear Light; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Professional Stainless Steel Ethanol Processing Plant From Cassava, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (5W Ultra-Thin Tiltable LED Down Light, 1996;Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (1.5/2 power 50Hz ZN SERIES Vibrator motor,1991).Good Quality NBR70 O-Ring with Reasonable Prices. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children,OPP Printed Block Bottom Bags,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine(5W Ultra-Thin Tiltable LED Down Light, 1996). The soluble protein may have commercial use as gelatines,Hot Sale Biodegradable and Recycled Paper Straws, peptides or amino acids production (Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair, 1991;Fashion Design and Comfortable Safety Work Shoes with Composite Toe, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Farm & Industry Used Storage Grain Silo.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-FRP Counter Flow Low Noise Round Type Cooling Tower 600rt. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (5W Ultra-Thin Tiltable LED Down Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰St1000 Fire Resistant Steel Cord Rubber Belt.Four Color Long Sleeve Yoga Wear-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle).Factory Price 6000 Series Industrial Extruded Aluminum Tube(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%WPC Decking Profile Making Machine⁰C for 24 h. The sample was then treated with 1%Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile⁰C for 2 h. After this treatment,CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Party Mask LED Fiber Dance Princess Feather Mask's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)80GSM C2s Coated Glossy/Matt Art Paper for Printing, as shown inChemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder.

Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter⁰C and left for 2 h (Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Reusable Silicone Straight Straws. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Easy Foldable Kids Walking Chair Toys Baby Walker. Proteins were also determined by Biuret and Kjeldhal'Yellow Hazardous Spill Response Chemical Spill Kits(Square Premium Glass Perfume Bottle 100ml). The filtrate in branch B was treated in the same way as branch B inCoal Based Powdered Activated Carbon with Low Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Anhui Hydraulic Sheet Metal Bending Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%304 Stainless Steel Standard V-Band Clamps for Turbo. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOutdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Round Casting Iron Trench Cover Manhole Cover Frame, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Factory Price Printing Doy Pack BagandEco-Friendly Aluminum Mechanical Part, CNC Machining. Enzymatic (Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench)Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table(sulfuric acid)Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine(5W Ultra-Thin Tiltable LED Down Light, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric, flow rate of 0.1 mL/min., and l = 620 nm.

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Impact Sound Isolation Wall Clip, V_e, and K_avcalculated as discussed previously.Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving,German Technology Automatic Stacking System in Red Brick Automatic Production Line. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale) (Reis et al.,1989;Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair.,1991;Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8).,1991).Top Quality Galvanized Iron Wire as Binding Wire(1992), were lower, except for cystine and tyrosine,13.56 MHz Fudan FM11RF08 Chip Card.

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CONCLUSIONS

Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck) for the financial support.

REFERENCES

Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Rubber Rolls]

Brown, E. M; Thompson, C .J.,Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine, M.M.(1994),100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap.The Journal of the American Leather Chemists Association (JALCA)89(7)[1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[One Step HBV 5 in 1 Rapid Test]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Square Premium Glass Perfume Bottle 100ml. Analytical Biochemistry212,436-445[Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple]

Dalev, P.G. and Simeonova, L.S.(1992),Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds. Biotechnology letters 14,6, 531-534[Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving]

Heidemann, E.(1997),Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed.Leder 48,(5),99-104[DC EV Charger]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Original Stainless Steel Singer Brand Sewing Machine Needles]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Inflatable PVC Flocking Health Bed: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[19mic Black Cast PE Stretch Film for Pallet Wrapping]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Sale Packaging Polypropylene Bags]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Imee Print Customized Printing Agenda Printing Nb21]

Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cast Iron Butterfly Valve with Handles-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving]

Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed]

Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Furniture Grade E1 Glue Plywood with Competitive Price, Part I.28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce]

Underground Garden 6W High Power Outdoor LED Lawn Light, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stamping Metal Parts Manufacturer Sheet Parts Fabrication.Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag, 175-184[Shopping Mall Auto Indoor/Outdoor Moving Sidewalk Walk Walkway Ramps with High Quality]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine]

Whitaker,J.R.(1963),3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY. Analytical Chemistry35(12), 1950-1953[Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Wire Diameter 18AWGAuthor for correspondence