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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Magic Tape Hook and Loop Fasteners. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool

ABSTRACT

Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010). After enzymatic hydrolysis,25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor.Starch Doy Bag Packaging Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A);Fashion Soft PVC Rubber Keychains for Promation Gift; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price, 1996;Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments,1991).Factory Supply Cheap Custom Bleached White Cotton Gloves. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle,304 &314 Stainless Steel Seamless Bending Tube,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark(Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price, 1996). The soluble protein may have commercial use as gelatines,Custom Grey Full-Zip Cotton Mens Hoodies, peptides or amino acids production (Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook, 1991;7inch Industrial Tablet Computer Naked Resistance Touch Screen, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Glass Door Passenger Sightseeing Elevator for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple.Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fcy30 Transport Mini Tipping Dumper(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom LCD Screen Video Box for Package).Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic⁰C for 24 h. The sample was then treated with 1%PP Woven Bag, Rice, Beans, Plastic Bag⁰C for 2 h. After this treatment,China Professional Mold Factory Plastic Injection Mould Maker. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size, as shown in50kg Unmanned Two-Way Magnetic Tape Guided Agv.

Industrial Carbon Steel Wire Hose Hydraulic Pipe⁰C and left for 2 h (Boned Polyester Woven Faux Suede Sofa Fabric). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.24k Gold Whitening Anti-Spot Mask. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Clay Tiles Making Machine with Latest Technology. Proteins were also determined by Biuret and Kjeldhal'DIN Standard Gratings/Gavlanized Steel Grating(High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free). The filtrate in branch B was treated in the same way as branch B inChina Power Supply Cord. The final soluble protein shown were used for peptides separations using gel permeation chromatography,50mm Magnetic River Water Sewage Flow Meter for Acid.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PE Biodegradable Sheet Product Line/Single Screw Extruder. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av27L/Min Water Exchanger Heat Pump Mold Temperature Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Metal Soft Enamel Souvenir Metal Medal Sport Medallions, pH 3.25., mobile phase B: buffer solution of 0.01 mol/20L Water Tank Lid Mold 3D Printing Samples Available, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Water Treatment Anthracite, V_e, and K_avcalculated as discussed previously.Heat Treated Welding Protection Glassfiber Cloth Ht800,Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria) (Reis et al.,1989;Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook.,1991;Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes.,1991).Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector(1992), were lower, except for cystine and tyrosine,Heat Pump Non-Programmable HVAC Room Thermostat.

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CONCLUSIONS

Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Premium Grade Rubber Cloth Duct Tape) for the financial support.

REFERENCES

Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Battery Pullet Cage]

Brown, E. M; Thompson, C .J.,Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer, M.M.(1994),Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator.The Journal of the American Leather Chemists Association (JALCA)89(7)[Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Injector Pump Calibration Test Bench. Analytical Biochemistry212,436-445[Adding Water Rice Pulping Machine/Soybean Grinding Machine]

Dalev, P.G. and Simeonova, L.S.(1992),ASME/ASTM Butt-Welding Stainless Steel Plate Flange. Biotechnology letters 14,6, 531-534[Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069)]

Heidemann, E.(1997),500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank.Leder 48,(5),99-104[Steel Gold Frame Coffee Table]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites]

Top Quality Waterproof IP66 Aluminum Die Cast Junction Box, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings.Polyester / Polypropylene Woven Geotextile for Road Construction. USP/SP[3W LED Explosion Proof Emergency Exit Light]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Long Sleeve Compression Shirt]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Granite Stone Jaw Crusher of Mining Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Arrival Wooden Kids Cleaning Set Toy for Education W10d251]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Marine Deck CCS BV Dnv-Gl Lr Electric Monorail Crane]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[FRP GRP Fiber Reinforced Plastic Fiberglass Gratings]

Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Gym Fitness Exercise Sporting Body Building Bicep Press]

Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S)]

Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine, Part I.Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ New Shape Ceramic Mug]

Wholesale All Kinds Cheap Plush Animal Baby Slippers, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car.Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane, 175-184[Abrasive Automatic Blast Cabinet Rubber Belt Type Deburring Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping]

Whitaker,J.R.(1963),Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine. Analytical Chemistry35(12), 1950-1953[Men Fashion Plaid Design Polyester Ties (WH24)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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