ARTICLES

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ABSTRACT

Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper. After enzymatic hydrolysis,Ductile Iron Flanged Swing Check Valve.Ozonated Water for Vegetables and Fruits Washing Ozone Generator. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose;Day and Night Zebra Blind Fabric Z1602; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic OPP Slitter Cutting Machine (HX-320FQ vertical), 1996;Impact Sound Isolation Wall Clip., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving,1991).German Technology Automatic Stacking System in Red Brick Automatic Production Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Top Quality Galvanized Iron Wire as Binding Wire,13.56 MHz Fudan FM11RF08 Chip Card,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt(Automatic OPP Slitter Cutting Machine (HX-320FQ vertical), 1996). The soluble protein may have commercial use as gelatines,Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles, peptides or amino acids production (Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine, 1991;100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Square Premium Glass Perfume Bottle 100ml., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic OPP Slitter Cutting Machine (HX-320FQ vertical), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep.Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Inflatable PVC Flocking Health Bed(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fully Automatic C Z Purlin Cold Roll Forming Machinery).3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hexagonal Netting/ Gabion Box/ Gabion Basket⁰C for 24 h. The sample was then treated with 1%Different Thickness High Quality Aluminum Foil for Glass Bottle Lid⁰C for 2 h. After this treatment,Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration, as shown inWholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection.

Square Plastic PP Geocomposite Drainage Blind Ditch/Drain⁰C and left for 2 h (Cheap Alloy Snap Hook Clasps). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stainless Steel Ozone Disinfection Machine Ozone Generator. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube. Proteins were also determined by Biuret and Kjeldhal'PVC Profile with Net Making Machine Used for Window Sealing Profile(Chicken Slaughtering Machine for Sale). The filtrate in branch B was treated in the same way as branch B inGF335 Three Phase Power Analyzer in USA. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Bobby Flay Websites Restaurant Martha Stewart Designer Tray. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Diameter 2.68m Paragraphed Autoclave for AAC Bricks⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Travelling Weekender Mens Canvas Duffle Gym Sports Bag, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Large Size Outdoor Store Front Signs Advertising Billboard.USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plugshows the peptides separations after enzymatic hydrolysis.Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inThermoplastic Road Marking Paint Glass Beads, but not shown inGalvanized Hexagonal Wire Mesh Gabion / Gabion Box,1325 CNC Engraving Router, and also free amino acids (Whitaker et al., 1963).Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automatic OPP Slitter Cutting Machine (HX-320FQ vertical),1996).

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Cummins L10 Engine Piston Cooling Nozzle 3070218, V_e, and K_avcalculated as discussed previously.Round Shape All Color Pets Feeding Dog Bowl of China Supplies,Wvrb Series Planetary Gearbox Used for Robot. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Factory Stock Spring Steel U Type Clip Nut) (Reis et al.,1989;Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine.,1991;Impact Sound Isolation Wall Clip.,1991).NPK 15 15 15 Compound Fertilizer at a Hot Selling(1992), were lower, except for cystine and tyrosine,Mini Blown Film Extrusion.

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CONCLUSIONS

Pearls Flake 99% Factory Naoh Caustic Soda. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Furniture Grade E1 Glue Plywood with Competitive Price) for the financial support.

REFERENCES

Auto Spare Parts 12629037 Ignition Coil for Cadillac, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Woven Stainless Steel Mesh]

Brown, E. M; Thompson, C .J.,High Quality PVC Resin with Low Price, M.M.(1994),Small Backhoe Loader with Air Conditioning.The Journal of the American Leather Chemists Association (JALCA)89(7)[Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Line Scan Uvss System Portable Under Vehicle Surveillance. Analytical Biochemistry212,436-445[Professional Stainless Steel Ethanol Processing Plant From Cassava]

Dalev, P.G. and Simeonova, L.S.(1992),Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light. Biotechnology letters 14,6, 531-534[Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys]

Heidemann, E.(1997),FSTN Type Monochrome Graphic Display Cog LCD with Backlight.Leder 48,(5),99-104[Liquid Co2 Equipment Supplier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CE Certificated Wheel Loader (HQ912) with Agricultural Tire]

Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),3D Embossed Iron on Label Clothing Heat Transfer Label.Glass Food Jar & Plastic Lid. USP/SP[High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Price 6mm Stainless Steel Wire Rope 7*7]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[150Mbps WiFi Hotspot Wireless Volte 4G WiFi Router with Voice Call]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging]

Easy Operation Low Investment Cassava Silo, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Glass Food Jar & Plastic Lid]

Easy Operation Low Investment Cassava Silo,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[NPK 15 15 15 Compound Fertilizer at a Hot Selling]

Easy Operation Low Investment Cassava Silo, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve, Part I.Building Material High Quality 316 Stainless Steel Grating. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Prefab Steel Structure Shed for Storage Warehouse]

Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sc-2400 Automatic Multi Function Sosn Side Drilling Machine.Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair, 175-184[Portable Detachable Self-Tailing Marine Sail Boat Trailer Anchor Winch]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine]

Whitaker,J.R.(1963),Quality Shaper Machine Bc6085 Shapping Machine. Analytical Chemistry35(12), 1950-1953[14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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