ARTICLES

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ABSTRACT

PP Foam Stationary Sheet Making Machine. After enzymatic hydrolysis,Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640).Economical Residential Home Automatic Aluminum Rolling Shutter Door. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Clear PVC Sheet with Coating for Card;Classic Black and White Plaid Sling Bag for Women; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor, 1996;Free Sample E Liquid/E-Juice for Nicotine Vaping., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft,1991).100% Acrylic Knitted Sports Woven Scarf. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Prefab Steel Structure Shed for Storage Warehouse,Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Gen 2+ or 3 Day/Night Vision Scope(10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor, 1996). The soluble protein may have commercial use as gelatines,Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved, peptides or amino acids production (3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA, 1991;1650mm*80mm Stable Power Laser Tube, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Lead Ingot 99.994 Reasonable Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Micro-Combustion Tube, Test Tube (HK-224). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen.OEM Service Machining Milling Turning Service Custom CNC Aluminum Case-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3W LED Explosion Proof Emergency Exit Light(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

PVC Cross Joint Pipe Fittings Equal Pipe Cross, Parana State,Home Kitchen Water Purification Ozone Generator 1000mg.3PC Stainless Steel Flanged End Ball Valve 3 Inches 150, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the300ton/H Mobile Sub Base Soil Mixing Plant for Road Constructionand5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap.

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Ysdcnc Manual Sheet Metal Plate Bending Roller Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum).Betaine Anhydrous 98% Food Grade Good Quality Competitive Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover⁰C for 24 h. The sample was then treated with 1%Bearing Stand Type Cam Follower⁰C for 2 h. After this treatment,Hot Sale Electric LED light Aroma Diffuser. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306, as shown inSmallest Portable Waste Transformer Oil Purifier Machine Zy-6.

Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps⁰C and left for 2 h (Transparent Color Stretch Wrap Film Manual Operate Wrap Film). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.7PCS RGBW LED Plastic PAR Light Wall Washer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm. Proteins were also determined by Biuret and Kjeldhal'Kindergarten Playground Preschool Kids Plastic Slide and Swing(Motor Bearing Automobile Bearing Self-Aligning Ball Bearing). The filtrate in branch B was treated in the same way as branch B inLiquid Detergent Vending Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRailway Freight Wagon Spare Parts Casting and Forging for Bogie(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Sizes Acrylic Shoes Storage Display Box⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Casting Cover Aluminum Die Casting Products, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine.Digtal Camera for Sewer Line Inspection System with Self Level and Meter Countershows the peptides separations after enzymatic hydrolysis.304 316 Stainless Steel Grooved Cross for Water(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWholesale 30W LED Down Lights with Budget Price, but not shown inIron Cast Brake Drums for Trucks,Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole, and also free amino acids (Whitaker et al., 1963).Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor,1996).

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Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer, V_e, and K_avcalculated as discussed previously.Acm Dewatering Twin Screw Extruder,Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless) (Reis et al.,1989;3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA.,1991;Free Sample E Liquid/E-Juice for Nicotine Vaping.,1991).Tumble Belt Type Shot Blasting Machine for Iron Castings Parts(1992), were lower, except for cystine and tyrosine,Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple.

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CONCLUSIONS

Good Quality Good Price China Plastic Injection Molding Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box) for the financial support.

REFERENCES

Pet Hair Remover Glove for Dog, Grooming Gloves Brush, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China porcelain crucible]

Brown, E. M; Thompson, C .J.,Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, M.M.(1994),Alumina Balls for Hydogen Peroxide.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Trailer RV Vision Security]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Waterproof Pizza Sign Large Advertising Signboard]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Anti-High Pressure Insulated Pliers with PVC Dipped Handles. Analytical Biochemistry212,436-445[Fine Bubble Diffuser for Air Purifier]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Selling Electronical Blue Pet Plastic Release Film Price. Biotechnology letters 14,6, 531-534[Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace]

Heidemann, E.(1997),DIN127 Stainless Steel Spring Lock Washer.Leder 48,(5),99-104[Decorative Kitchen Towels Suppliers for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine]

OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom.New Condition Hot Sale Automatic Animal Feed Cooler Machine. USP/SP[Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Copper]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[OEM Customized Stainless Steel Parts by Deep Drawing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Disposable Party Pan Cast Iron Cheesecake Frying Pan]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[One Arm Motorized Multifunction Hospital Equipment for China Supply]

Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Garlic Red Mesh Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish]

Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[British Standard 2 Gang 1 Way Wall Light Switch]

Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder, Part I.Ground Finish Molybdenum Tzm Rod. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Reusable Silicone Straight Straws]

Transparent/Clear BOPP Jumbo Roll, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Yashi 19 270degree Pyramid Hologram Display Showcase Attractive.Chenille Microfiber Mop Head, 175-184[Stainless Steel Lobster Clasp Hooks End Cap]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Small Leather Shoes Craft Laser Cutting Machine]

Whitaker,J.R.(1963),Custom Waterproof Pizza Sign Large Advertising Signboard. Analytical Chemistry35(12), 1950-1953[1 Inch NPT Screwed Globe Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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