ARTICLES

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ABSTRACT

Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft. After enzymatic hydrolysis,100% Acrylic Knitted Sports Woven Scarf.Prefab Steel Structure Shed for Storage Warehouse. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture;Gen 2+ or 3 Day/Night Vision Scope; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA, 1996;1650mm*80mm Stable Power Laser Tube., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit,1991).High Quality Lead Ingot 99.994 Reasonable Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Micro-Combustion Tube, Test Tube (HK-224),Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,OEM Service Machining Milling Turning Service Custom CNC Aluminum Case(3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA, 1996). The soluble protein may have commercial use as gelatines,3W LED Explosion Proof Emergency Exit Light, peptides or amino acids production (Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making, 1991;Stationery PP Clipboard With 7 Inch Scale Ruler, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PVC Cross Joint Pipe Fittings Equal Pipe Cross. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰3PC Stainless Steel Flanged End Ball Valve 3 Inches 150.Ysdcnc Manual Sheet Metal Plate Bending Roller Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Betaine Anhydrous 98% Food Grade Good Quality Competitive Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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7PCS RGBW LED Plastic PAR Light Wall Washer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Wholesale Hot Sale Rubber Silicone Seal Diaphragm).750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Kindergarten Playground Preschool Kids Plastic Slide and Swing⁰C for 24 h. The sample was then treated with 1%Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks⁰C for 2 h. After this treatment,Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Railway Freight Wagon Spare Parts Casting and Forging for Bogie's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Rainsky Ceiling Mounted Stainless Steel Shower Heads, as shown inVertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine.

Custom Sizes Acrylic Shoes Storage Display Box⁰C and left for 2 h (Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Casting Cover Aluminum Die Casting Products. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags. Proteins were also determined by Biuret and Kjeldhal'304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser(Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens). The filtrate in branch B was treated in the same way as branch B inSlip Silk Pillowcase for Hair 22 Momme 6A 100% Mulberry Silk Oeko Verified. The final soluble protein shown were used for peptides separations using gel permeation chromatography,SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFurniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/304 316 Stainless Steel Grooved Cross for Water, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Acm Dewatering Twin Screw Extruder.Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mouldshows the peptides separations after enzymatic hydrolysis.Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale, but not shown in3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls,Tumble Belt Type Shot Blasting Machine for Iron Castings Parts, and also free amino acids (Whitaker et al., 1963).Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA,1996).

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Alumina Balls for Hydogen Peroxide, V_e, and K_avcalculated as discussed previously.Anti-High Pressure Insulated Pliers with PVC Dipped Handles,Hot Selling Electronical Blue Pet Plastic Release Film Price. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine) (Reis et al.,1989;Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making.,1991;1650mm*80mm Stable Power Laser Tube.,1991).DIN127 Stainless Steel Spring Lock Washer(1992), were lower, except for cystine and tyrosine,29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom.

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CONCLUSIONS

New Condition Hot Sale Automatic Animal Feed Cooler Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale Electric LED light Aroma Diffuser) for the financial support.

REFERENCES

Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Steam Globe Valve]

Brown, E. M; Thompson, C .J.,Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144), M.M.(1994),Garlic Red Mesh Bag.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Plastic Toys]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wvrb Series Planetary Gearbox Used for Robot]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder. Analytical Biochemistry212,436-445[08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain]

Dalev, P.G. and Simeonova, L.S.(1992),Ground Finish Molybdenum Tzm Rod. Biotechnology letters 14,6, 531-534[Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages]

Heidemann, E.(1997),Transparent/Clear BOPP Jumbo Roll.Leder 48,(5),99-104[Oil Immersed Transformer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Free Sample! AC Power Contactor for Sale]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Business Class Eyeshade Quality Travel Eye Mask Sleeping Eye Cover]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Waterproof Pizza Sign Large Advertising Signboard: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Orfs Male/Orfs Female Hose Fitting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Suction Function Handheld Cool and Heat Food Bowl for Baby]

PVC Shrink Sleeve Label for Drinking Water Bottle, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Shacman F3000 6X4 Truck Trailer Head]

PVC Shrink Sleeve Label for Drinking Water Bottle,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[WPC Decking Profile Making Machine]

PVC Shrink Sleeve Label for Drinking Water Bottle, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Galvanized Square Wire Mesh, Metal Window Screen, Part I.Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet]

2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM Customized Stainless Steel Parts by Deep Drawing.Children Bear English Story Book Printing, 175-184[BBQ Grill Rv′s Trailer Propane Gas Extension Hose 1/4NPT Ball Fitting Flexible Pipe Hoses]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Good Quality NBR70 O-Ring with Reasonable Prices]

Whitaker,J.R.(1963),Flexible Board FPC PCB Camera Module Rigid Flex PCB. Analytical Chemistry35(12), 1950-1953[Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024Author for correspondence