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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Eco-Friendly Aluminum Mechanical Part, CNC Machining. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench

ABSTRACT

Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table. After enzymatic hydrolysis,Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine.Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper;Ductile Iron Flanged Swing Check Valve; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ozonated Water for Vegetables and Fruits Washing Ozone Generator, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose, 1996;Day and Night Zebra Blind Fabric Z1602., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock,1991).Automatic OPP Slitter Cutting Machine (HX-320FQ vertical). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Impact Sound Isolation Wall Clip,Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,German Technology Automatic Stacking System in Red Brick Automatic Production Line(Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose, 1996). The soluble protein may have commercial use as gelatines,Top Quality Galvanized Iron Wire as Binding Wire, peptides or amino acids production (13.56 MHz Fudan FM11RF08 Chip Card, 1991;Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Square Premium Glass Perfume Bottle 100ml.Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Cast Iron Butterfly Valve with Handles, Parana State,Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench.Furniture Grade E1 Glue Plywood with Competitive Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFire Extinguisher UV Curing Machine Cylinder Drying Machineand700mm RP HP UHP High Quality Graphite Electrode.

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28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine).Underground Garden 6W High Power Outdoor LED Lawn Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stamping Metal Parts Manufacturer Sheet Parts Fabrication⁰C for 24 h. The sample was then treated with 1%Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag⁰C for 2 h. After this treatment,3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hexagonal Netting/ Gabion Box/ Gabion Basket's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Different Thickness High Quality Aluminum Foil for Glass Bottle Lid, as shown inVertical Automatic Granule Bag Packing Machine.

Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla⁰C and left for 2 h (Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration. Proteins were also determined by Biuret and Kjeldhal'Square Plastic PP Geocomposite Drainage Blind Ditch/Drain(Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal). The filtrate in branch B was treated in the same way as branch B inBest Beacons To Buy Supplier Best-Selling. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Ozone Disinfection Machine Ozone Generator.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPVC Profile with Net Making Machine Used for Window Sealing Profile(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Bobby Flay Websites Restaurant Martha Stewart Designer Tray⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Modern Living Room Furniture Hotel Bedroom Custom Corner SofaandDiameter 2.68m Paragraphed Autoclave for AAC Bricks. Enzymatic (Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety)Travelling Weekender Mens Canvas Duffle Gym Sports Bag(sulfuric acid)4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box(Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC), flow rate of 0.1 mL/min., and l = 620 nm.

Best Selling Household Living Room Mat Chenille Carpets. Damask Pillows Covershows the peptides separations after enzymatic hydrolysis.130HP 4WD Large Farm Power Engine Tractor From High Quality Factory(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHf Vacuum Dryer Machine with Advanced High Frequency Technology, but not shown in1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction,Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment, and also free amino acids (Whitaker et al., 1963).Large Size Outdoor Store Front Signs Advertising Billboard. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose,1996).

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CONCLUSIONS

Wvrb Series Planetary Gearbox Used for Robot. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Inflatable PVC Flocking Health Bed) for the financial support.

REFERENCES

NPK 15 15 15 Compound Fertilizer at a Hot Selling, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Anti Static Gloves]

Brown, E. M; Thompson, C .J.,Mini Blown Film Extrusion, M.M.(1994),Pearls Flake 99% Factory Naoh Caustic Soda.The Journal of the American Leather Chemists Association (JALCA)89(7)[2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Auto Spare Parts 12629037 Ignition Coil for Cadillac. Analytical Biochemistry212,436-445[Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality PVC Resin with Low Price. Biotechnology letters 14,6, 531-534[PVC Ceiling Wall Pannelling]

Heidemann, E.(1997),Small Backhoe Loader with Air Conditioning.Leder 48,(5),99-104[Ladies Hooded Rain Wear Long Windbreaker Jacket]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Furniture Grade E1 Glue Plywood with Competitive Price]

Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Line Scan Uvss System Portable Under Vehicle Surveillance.Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light. USP/SP[Round Type Twin Screw Extruder Barrel Manufacturer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-FSTN Type Monochrome Graphic Display Cog LCD with Backlight: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Embossed Textured Wallpaper for Solvent Printing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Free Samples 1575.42MHz 3.5dB Passive Built-in Wireless GPS Ceramic Antenna]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Powerful Full Hydraulic Safework Certificates Hgy13 15 17m Mobile Concrete Placing Booms]

3D Embossed Iron on Label Clothing Heat Transfer Label, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Glass Food Jar & Plastic Lid-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Injector Pump Calibration Test Bench]

3D Embossed Iron on Label Clothing Heat Transfer Label,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cashew Grading Machine for Cashew Processing Line]

3D Embossed Iron on Label Clothing Heat Transfer Label, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer, Part I.Easy Operation Low Investment Cassava Silo. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Grooming Gloves Brush]

CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve.Building Material High Quality 316 Stainless Steel Grating, 175-184[Fluorescent Attachment for Microscope]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Travelling Weekender Mens Canvas Duffle Gym Sports Bag]

Whitaker,J.R.(1963),Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table. Analytical Chemistry35(12), 1950-1953[Aluminum Truss System]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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