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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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Large Size Outdoor Store Front Signs Advertising Billboard. After enzymatic hydrolysis,Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg.1325 CNC Engraving Router. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel;T6s 86 PCD Core Bit for Geotechnical Drilling; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CNC Machining 6601 Aluminum Parts with Anodized Automoblile, 1996;Cummins L10 Engine Piston Cooling Nozzle 3070218., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Round Shape All Color Pets Feeding Dog Bowl of China Supplies,1991).Wvrb Series Planetary Gearbox Used for Robot. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,NPK 15 15 15 Compound Fertilizer at a Hot Selling,Mini Blown Film Extrusion,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pearls Flake 99% Factory Naoh Caustic Soda(CNC Machining 6601 Aluminum Parts with Anodized Automoblile, 1996). The soluble protein may have commercial use as gelatines,Auto Spare Parts 12629037 Ignition Coil for Cadillac, peptides or amino acids production (High Quality PVC Resin with Low Price, 1991;Small Backhoe Loader with Air Conditioning, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Line Scan Uvss System Portable Under Vehicle Surveillance., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-FSTN Type Monochrome Graphic Display Cog LCD with Backlight. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CNC Machining 6601 Aluminum Parts with Anodized Automoblile, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 103D Embossed Iron on Label Clothing Heat Transfer Label.Glass Food Jar & Plastic Lid-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


Easy Operation Low Investment Cassava SiloAlcalase2.5 L DX,CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Building Material High Quality 316 Stainless Steel Grating(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table, Parana State,Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment.Sc-2400 Automatic Multi Function Sosn Side Drilling Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (CNC Machining 6601 Aluminum Parts with Anodized Automoblile, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAdding Water Rice Pulping Machine/Soybean Grinding MachineandZinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069).

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Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (3W LED Explosion Proof Emergency Exit Light).Quality Shaper Machine Bc6085 Shapping Machine(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Thermoplastic Road Marking Paint Glass Beads0C for 24 h. The sample was then treated with 1%Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine0C for 2 h. After this treatment,Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5, as shown in Long Sleeve Compression Shirt.

Free Sample! AC Power Contactor for Sale0C and left for 2 h (New Arrival Wooden Kids Cleaning Set Toy for Education W10d251). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.PA System Loudspeaker 100W Outdoor Column Speaker . A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel. Proteins were also determined by Biuret and Kjeldhal'Breathable Surgical Disposable 3 Ply Earloop Face Mask(FRP GRP Fiber Reinforced Plastic Fiberglass Gratings). The filtrate in branch B was treated in the same way as branch B inCalprotectin Blood Test High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavCe Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Design Indoor Artificial Mini Rockery Fountains0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Medical Endoscope Disposable Bite Block with Bandage, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Gym Fitness Exercise Sporting Body Building Bicep PressandAluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels. Enzymatic (Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08))Ladder Type Cable Tray with Ce/ GOST/ TUV/UL(sulfuric acid)12V Electric Portable Outdoor Water Fruit Car Fridge with Ce(CNC Machining 6601 Aluminum Parts with Anodized Automoblile, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Fine Bubble Diffuser for Air Purifier, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Fine Bubble Diffuser for Air Purifier, flow rate of 0.1 mL/min., and l = 620 nm.

Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore.1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S)shows the peptides separations after enzymatic hydrolysis.High Quality Cheap Price Custom Aluminum Logo Metal Label Plate(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in New Shape Ceramic Mug, but not shown inPortable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping,3120/17/38 Boltable Crane Rail Clips, and also free amino acids (Whitaker et al., 1963).2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CNC Machining 6601 Aluminum Parts with Anodized Automoblile,1996).

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Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key ShellandDouble-Offset Double Flanged Stainless Steel Butterfly Valveshow the gel chromatographic separation of soluble protein with Sephadex G-China Supplier Rubber Injection Molding Machine with Ce.Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China, and another peak at 45 mL not shown in7inch Industrial Tablet Computer Naked Resistance Touch Screen, for peptides lower than 3000 Da.Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery(Condell et al.,1993) .

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High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free, Ve, and Kavcalculated as discussed previously.Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag,Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors ( Relfective Plastic Reflector) (Reis et al.,1989;High Quality PVC Resin with Low Price.,1991;Cummins L10 Engine Piston Cooling Nozzle 3070218.,1991).High Quality & High Speed Straight Line Wire Drawing Machine(1992), were lower, except for cystine and tyrosine,High Speed 3000W Fiber Laser Cutter.

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Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve) for the financial support.


3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Narrow Fabric etc]

Brown, E. M; Thompson, C .J.,Personality Cat Eye Sunglasses, M.M.(1994),ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Sizes Acrylic Shoes Storage Display Box]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-UV Coated PVC Panel for Interior Wall Board. Analytical Biochemistry212,436-445[Granite Stone Jaw Crusher of Mining Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.. Biotechnology letters 14,6, 531-534[22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet]

Heidemann, E.(1997),Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device.Leder 48,(5),99-104[Hot-Dip Galvanized Nut]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[70m Series Aluminiun Plastic Coated Profile for Widnows]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Protection Equipment for Face Mask Full Face Respirator Full Face Gas Mask]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration]

4-14 Manual Staple Gun with Staples for Hand Tools, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles]

4-14 Manual Staple Gun with Staples for Hand Tools,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale Power Press Perforated Metal Punch Machine]

4-14 Manual Staple Gun with Staples for Hand Tools, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Smallest Portable Waste Transformer Oil Purifier Machine Zy-6, Part I.Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels]

High Speed Automatic Packing Machine (GK-650 BA), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),5000-5900MHz 4G Built-in Automotive Antenna.Glass Lined Reactor for Chemical Factory, 175-184[Binding Post Terminal Electrical Factory OEM/ODM Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt]

Whitaker,J.R.(1963),OEM Metal Stamping Stainless Steel Parts with CNC Machining. Analytical Chemistry35(12), 1950-1953[Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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