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ABSTRACT

XL-21 Type OEM Motive Electrical Switchgear Power Cabinet. After enzymatic hydrolysis,Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient.White HPL 600*600mm Coffee Table. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale;Factory Price 6000 Series Industrial Extruded Aluminum Tube; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.WPC Decking Profile Making Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile, 1996;CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Party Mask LED Fiber Dance Princess Feather Mask,1991).80GSM C2s Coated Glossy/Matt Art Paper for Printing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter,Reusable Silicone Straight Straws,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Easy Foldable Kids Walking Chair Toys Baby Walker(Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile, 1996). The soluble protein may have commercial use as gelatines,Yellow Hazardous Spill Response Chemical Spill Kits, peptides or amino acids production (Anhui Hydraulic Sheet Metal Bending Machine, 1991;304 Stainless Steel Standard V-Band Clamps for Turbo, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Round Casting Iron Trench Cover Manhole Cover Frame.Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Eco-Friendly Aluminum Mechanical Part, CNC Machining(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Professional Gym Equipment Hammer Strength PRO Style Adjustable BenchAlcalase2.5 L DX,Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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Ozonated Water for Vegetables and Fruits Washing Ozone Generator(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Frozen Soybean in Pod).Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Day and Night Zebra Blind Fabric Z1602⁰C for 24 h. The sample was then treated with 1%40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock⁰C for 2 h. After this treatment,Automatic OPP Slitter Cutting Machine (HX-320FQ vertical). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Impact Sound Isolation Wall Clip's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving, as shown inProfessional Handheld Underground Gold Metal Detector Waterproof Pinpointer.

German Technology Automatic Stacking System in Red Brick Automatic Production Line⁰C and left for 2 h (Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Top Quality Galvanized Iron Wire as Binding Wire. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,13.56 MHz Fudan FM11RF08 Chip Card. Proteins were also determined by Biuret and Kjeldhal'Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt(Punching Bending Welding Steel Work Fabrication). The filtrate in branch B was treated in the same way as branch B inStainless Steel Water Filter Under Sink. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer.Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipmentshows the peptides separations after enzymatic hydrolysis.Underground Garden 6W High Power Outdoor LED Lawn Light(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDecor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring, but not shown in304 &314 Stainless Steel Seamless Bending Tube,Stamping Metal Parts Manufacturer Sheet Parts Fabrication, and also free amino acids (Whitaker et al., 1963).Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile,1996).

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Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla, V_e, and K_avcalculated as discussed previously.Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages,Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Solid Wood Buffet Storage Cupboard Table Designs Cabinet Sideboard) (Reis et al.,1989;Anhui Hydraulic Sheet Metal Bending Machine.,1991;CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories.,1991).Square Plastic PP Geocomposite Drainage Blind Ditch/Drain(1992), were lower, except for cystine and tyrosine,Stainless Steel Ozone Disinfection Machine Ozone Generator.

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CONCLUSIONS

Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine) for the financial support.

REFERENCES

PVC Profile with Net Making Machine Used for Window Sealing Profile, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[ISO Mobile Insulated Shipping Container]

Brown, E. M; Thompson, C .J.,Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch, M.M.(1994),Bobby Flay Websites Restaurant Martha Stewart Designer Tray.The Journal of the American Leather Chemists Association (JALCA)89(7)[Metal Soft Enamel Souvenir Metal Medal Sport Medallions]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Classic Black and White Plaid Sling Bag for Women]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line. Analytical Biochemistry212,436-445[ Black Diamond]

Dalev, P.G. and Simeonova, L.S.(1992),Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber. Biotechnology letters 14,6, 531-534[High Speed 3000W Fiber Laser Cutter]

Heidemann, E.(1997),Diameter 2.68m Paragraphed Autoclave for AAC Bricks.Leder 48,(5),99-104[Mudguard For Cars]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Digger Arm Small Wheel Loader (HQ908) with CE]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fashion Soft PVC Rubber Keychains for Promation Gift]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[49 Inch Shopping Mall Advertising Totem Touch Screen Kiosk]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Logo Fleece Hoodies Men Pullover Neno Oversized Men′s Hoodies]

China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Best Selling Household Living Room Mat Chenille Carpets-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale]

China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[27L/Min Water Exchanger Heat Pump Mold Temperature Machine]

China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:130HP 4WD Large Farm Power Engine Tractor From High Quality Factory, Part I.Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom All Kinds of Acrylic Shoe Display Racks]

Large Size Outdoor Store Front Signs Advertising Billboard, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg.1325 CNC Engraving Router, 175-184[China frog skeleton]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire]

Whitaker,J.R.(1963),Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel. Analytical Chemistry35(12), 1950-1953[Computerized Single Fishnet Jacquard Circular Knitting Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Real Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for WatchAuthor for correspondence