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ABSTRACT

C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L. After enzymatic hydrolysis,Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory.WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Poultry House, Livestock, Poultry Farm, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine, 1996;Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Black Slim PU Leather Passport Travel Wallet,1991).ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce,3 Colors Tactical Shooting Hunting Camouflage Clothes Suit,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1.3KG Fire breaker for kitchen(1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine, 1996). The soluble protein may have commercial use as gelatines,Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign, peptides or amino acids production (Korean Integrated CTO Activated Carbon Filter for Water Treatment, 1991;Vacuum Squeeze Filter Press for Coal Flotation Tailings, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Modular Portable Shipping Container Lkit Building Home Villa House., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Supplier of Excavator Spare Parts for Caterpillar Cat330. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal.Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wheel-Type-Carriage Precision Heavy Duty Linear Guide(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Popular European Style White Marble Coffee Tables for Dinnig Room. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Kwikstage Scaffolding System-Diagonal Brace⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/360 Rotating Automatic Touchless Car Wash Equipment with Drying System, pH 3.25., mobile phase B: buffer solution of 0.01 mol/48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Home Decoration Candle Glass Holder for Table Decorative) for the financial support.

REFERENCES

Custom Size Christmas Gift Personalized Watch Boxes for Men, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Level Gauge Accessories Water Level Indicator Roller Column Level Gauge]

Brown, E. M; Thompson, C .J.,12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope, M.M.(1994),8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector.The Journal of the American Leather Chemists Association (JALCA)89(7)[Bending Sheet Metal Custom-Made Aluminum Bracket]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pneumatic Air Driven Liquid Booster Pump]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality. Analytical Biochemistry212,436-445[Engine Parts Rubber High Quality Timing Belt 111ru25]

Dalev, P.G. and Simeonova, L.S.(1992),New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115. Biotechnology letters 14,6, 531-534[White HPL 600*600mm Coffee Table]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mini Blown Film Extrusion]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Professional Best Poly Mono Half Cell Solar Panel Suppliers]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer]

Kids Trucker Hat, Kids Mesh Cap, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wd190V Handheld Full Body Scanner Hand Held Metal Detector-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Rectangular Box Facial Tissue Paper Fk-110]

Kids Trucker Hat, Kids Mesh Cap,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1.75mm 3mm ABS PLA PC 3D Printer Filament Machine]

Kids Trucker Hat, Kids Mesh Cap, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed, Part I.Hydraulic Orbital Valve Power Steering. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads]

W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),AISI 410 Stainless Steel Galvanized Wire for Making Scourer.CS1363 a Standard UK 3 Pin Power Plug, 175-184[Precision Aluminum CNC Milling Machining Equipment Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Flexible Board FPC PCB Camera Module Rigid Flex PCB]

Whitaker,J.R.(1963),Food Chopper. Analytical Chemistry35(12), 1950-1953[Baking Soda 99.8% Min Sodium Bicarbonate]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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