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ABSTRACT

Square Premium Glass Perfume Bottle 100ml. After enzymatic hydrolysis,Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds.Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Inflatable PVC Flocking Health Bed, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence, 1996;Cast Iron Butterfly Valve with Handles., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Furniture Grade E1 Glue Plywood with Competitive Price,1991).28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Underground Garden 6W High Power Outdoor LED Lawn Light,Stamping Metal Parts Manufacturer Sheet Parts Fabrication,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag(Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence, 1996). The soluble protein may have commercial use as gelatines,3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY, peptides or amino acids production (Hexagonal Netting/ Gabion Box/ Gabion Basket, 1991;Different Thickness High Quality Aluminum Foil for Glass Bottle Lid, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Square Plastic PP Geocomposite Drainage Blind Ditch/Drain.Stainless Steel Ozone Disinfection Machine Ozone Generator-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Ceramic Chrome Hybrid Bearing Oil).Travelling Weekender Mens Canvas Duffle Gym Sports Bag(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box⁰C for 24 h. The sample was then treated with 1%China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC)⁰C for 2 h. After this treatment,Best Selling Household Living Room Mat Chenille Carpets. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)130HP 4WD Large Farm Power Engine Tractor From High Quality Factory's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment, as shown inBeauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment.

Large Size Outdoor Store Front Signs Advertising Billboard⁰C and left for 2 h (Good Price Welding Electrode (e7018/e7018-1)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,1325 CNC Engraving Router. Proteins were also determined by Biuret and Kjeldhal'Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel( Printed Socks). The filtrate in branch B was treated in the same way as branch B inCast Iron Pan Scrubber / Stainless Steel Chainmail Mesh Cleaner. The final soluble protein shown were used for peptides separations using gel permeation chromatography,T6s 86 PCD Core Bit for Geotechnical Drilling.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCNC Machining 6601 Aluminum Parts with Anodized Automoblile(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Round Shape All Color Pets Feeding Dog Bowl of China Supplies⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wvrb Series Planetary Gearbox Used for Robot, pH 3.25., mobile phase B: buffer solution of 0.01 mol/NPK 15 15 15 Compound Fertilizer at a Hot Selling, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Bobby Flay Websites Restaurant Martha Stewart Designer Tray) for the financial support.

REFERENCES

Quality Shaper Machine Bc6085 Shapping Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Air Pre Filter]

Brown, E. M; Thompson, C .J.,Thermoplastic Road Marking Paint Glass Beads, M.M.(1994),Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[China PA System Power Amplifier with Enough Rated Power]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sud1000h Poly Welder Butt Fusion Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts. Analytical Biochemistry212,436-445[Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers]

Dalev, P.G. and Simeonova, L.S.(1992),I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case. Biotechnology letters 14,6, 531-534[Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet]

Heidemann, E.(1997),Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5.Leder 48,(5),99-104[China Bathroom Mirror with Beveling Edge]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Activated Carbon Strong Effect Peeling off Face Mask Mud]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Nutrition Enhancer Environmental Nutritional Yeast Powder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[5g Ozone Generator Car Odor Remover for Air Sterilization]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile]

Breathable Surgical Disposable 3 Ply Earloop Face Mask, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W]

Breathable Surgical Disposable 3 Ply Earloop Face Mask,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Wireless Cheapest Android 2D Barcode Scanner]

Breathable Surgical Disposable 3 Ply Earloop Face Mask, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw, Part I.Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Full Automatic Carbonated Water Filling Full Line]

Flower Design Custom Made Silk Scarf, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),New Design Indoor Artificial Mini Rockery Fountains.7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane, 175-184[Plastic Wire Mesh Plastic Flat Net High Quality]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High-Quality Pre-Seasoned Cast Iron Wok]

Whitaker,J.R.(1963),Medical Endoscope Disposable Bite Block with Bandage. Analytical Chemistry35(12), 1950-1953[New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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