ARTICLES

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ABSTRACT

Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale. After enzymatic hydrolysis,Medical Supply U40 Disposable Insulin Syringe.Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Water Chiller Air Cooled Chiller Industrial Chiller for Sale;Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Precision Tungsten Carbide Machine Customized Cutting Tool, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box, 1996;Maleic Anhydride CAS 108-31-6., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2000mj Q Switched for Pigment Removal with Ce Certification,1991).Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cow Dung Chicken Manure Organic Fertilizer Making Machine,marble Makeup Brush Set for Travel in 20PCS,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Swimming Pool Water Disinfect Ozone Generator(I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box, 1996). The soluble protein may have commercial use as gelatines,20kv 50kVA High Voltage Dry Type Power Supply Transformer, peptides or amino acids production (Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp, 1991;High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;4 Styles Blue Glass Crystal Butt Beads Spiral Plug., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Industrial Activated Carbon Water Filter Industry Water Treatment. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2016 New Generation Sausage Making Machine.Magnetic Mixer Mixing Tank with Bottom Agitator (MTK)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt, Parana State,Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide.10ton Telescopic Hydraulic Truck Crane, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMineral Industry Magnet Ore Ball Milling MachineandCS1363 a Standard UK 3 Pin Power Plug.

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Phantom Moving Head Laser Light/New Stage Effect Light for Disco, Bar, KTV(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts).for iPhone 6 LCD 100% Brand New with Touch Screen Digitizer Assembly Replacement Display(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cracker Multi Rows Trayless Packaging Machine⁰C for 24 h. The sample was then treated with 1%Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China⁰C for 2 h. After this treatment,2 Quick Change Flexible Roloc Disc. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Aluminium Windows Thermal Break China Supplier, as shown inLED Bulb Light with Remote Control RGB Christmas Bulb.

Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines⁰C and left for 2 h ( Lgd4033). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2.0mm Pitch Pin Header SMT Type T Series Single Row Dual Housing B01. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,PRO-Installer Fiber Optic Termination Kit Lk-6016. Proteins were also determined by Biuret and Kjeldhal'Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace(Bluetooth Smart Watch with Heart Rate Monitor for Men 72087). The filtrate in branch B was treated in the same way as branch B inSpare Part For Machinery Factory & Manufacturer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPowder Coated H Column Beam Steel Structure Building for Sale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Nylon Cable Glands with CE, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Marine High Visibility Solas Reflective TapeandX Shaped Extruded Aluminum Window Screen Frames. Enzymatic (Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair)Sarms 99% Lgd-4033, Lgd4033, CAS 1165910-22-4(sulfuric acid)4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging(I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet, flow rate of 0.1 mL/min., and l = 620 nm.

Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD.Maleic Anhydride CAS 108-31-6shows the peptides separations after enzymatic hydrolysis.Low Power Consumption Laser Foam Vertical Die Cutting Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes, but not shown inAcm Dewatering Twin Screw Extruder,New Bone China Mug, 16oz Coffee Cup, New Shape Ceramic Mug, High Quality White Mug, and also free amino acids (Whitaker et al., 1963).Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouse. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box,1996).

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CONCLUSIONS

Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing) for the financial support.

REFERENCES

Flat Surface Mounted 300mm 24W LED Panel Light for Interior, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Plastic Shrink Black Stretch Wrap Film]

Brown, E. M; Thompson, C .J.,Latex Paint Emulsion Thickener Agents with High Leveling Property, M.M.(1994),Fresh Juice with Suagr Filling Bottling Machine Small Scale Low Prices.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Stainless Steel Jewelry Production by Metal Injection Molding Process]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[6.6kw LiFePO4 Charger for Lithium Battery Pack]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Orfs Male/Orfs Female Hose Fitting. Analytical Biochemistry212,436-445[Folding Aluminium Easy Move Choral Stage with Wheels for School]

Dalev, P.G. and Simeonova, L.S.(1992),Ss400 Galvanized Steel C Channel Steel Price. Biotechnology letters 14,6, 531-534[Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage]

Heidemann, E.(1997),Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping.Leder 48,(5),99-104[Laser Cutting Ceramic]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Superior Watertightness JS Polymer Cement Waterproof Coating for Basement]

Custom Logo Silicone Wristband, Rubber Wrist Bands, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter.Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple. USP/SP[New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Silicone Cake Mold Sinle Cupcake DIY]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Step Motor for Intelligent Sanitary/3D Printer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sludge Oil Separation Centrifuge]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Home and Office PVC Vinyl Flooring Conductive Plank Flooring]

81144m Thrust Roller Bearing Used for Vertical Machine Machinery, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fancy Veneer Plywood. Natural Veneer Plywood for Furniture-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pet Laminate Aluminium Foil/Pet+Alu+PE]

81144m Thrust Roller Bearing Used for Vertical Machine Machinery,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure]

81144m Thrust Roller Bearing Used for Vertical Machine Machinery, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating, Part I.Aluminum Foil Heat Shield Screen Matting Adhesive Backed. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing]

Home Kitchen Water Purification Ozone Generator 1000mg, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),PP Foam Stationary Sheet Making Machine.Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640), 175-184[yishanjiushe.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable]

Whitaker,J.R.(1963),Economical Residential Home Automatic Aluminum Rolling Shutter Door. Analytical Chemistry35(12), 1950-1953[Aluminum Foil Heat Shield Screen Matting Adhesive Backed]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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