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ABSTRACT

Single Board 3.7V~24VDC Microwave Detector PCB. After enzymatic hydrolysis,Automatic Chicken Nipple Drinker System.Industrial Hydraulic Ice Grape Press Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Glass Salt & Pepper Mill/Grinder Glass Spice Jar, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Outsole Trimming Machine, 1996;Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Shining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01),1991).CE Certificated Wheel Loader (HQ912) with Agricultural Tire. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts,PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet(Outsole Trimming Machine, 1996). The soluble protein may have commercial use as gelatines,Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump, peptides or amino acids production (ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment, 1991;Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Auto Two Piece Folder Gluer for Corrugated Box. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Outsole Trimming Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰British Standard 2 Gang 1 Way Wall Light Switch.Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire, Parana State,2010 Used Foton Truck Mixer.Rectangular Stainless Steel Pipe SS304, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Outsole Trimming Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGlass Door Passenger Sightseeing Elevator for SaleandYidek 3 Phase 4 Wire Automatic Digital Power Analysis Testing Analyzer.

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High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Half Nut).6.6kw LiFePO4 Charger for Lithium Battery Pack(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers⁰C for 24 h. The sample was then treated with 1%50m Depth Long Range Metal Detector with Reliable Quality⁰C for 2 h. After this treatment,CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Customized Stable Burn-in Test Equipment, as shown inFuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle.

Wholesale Custom 100% Cotton Boys T-Shirt with Print⁰C and left for 2 h (Bearing Stand Type Cam Follower). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Industry 0.002mm Accuracy Checking Fixture. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Promotional Customized PP Hand Fan, Plastic Folding Fan. Proteins were also determined by Biuret and Kjeldhal'450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting(Vacuum Squeeze Filter Press for Coal Flotation Tailings). The filtrate in branch B was treated in the same way as branch B inAluminum Composite Material Low Cost with Stable Quality. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Six Stations Pen Press Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avVertical Automatic Granule Bag Packing Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Cracker Multi Rows Trayless Packaging MachineandSmall Manufacturing Tht/ Ai Double Wave Soldering Machine. Enzymatic (Manual Sealing Machine for Instant Noodles Paper Soup Bowls)Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck(sulfuric acid)Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading(Outsole Trimming Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion, flow rate of 0.1 mL/min., and l = 620 nm.

Corrugated Steel Sheet Corrugated Steel Roofing Sheet.Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panelsshows the peptides separations after enzymatic hydrolysis.China Factory Queen Mattress with Visco Memory Foam and Latex(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System, but not shown inBottom Pushing for Fire Extinguisher Hot Spinning Machine,High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing, and also free amino acids (Whitaker et al., 1963).Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Outsole Trimming Machine,1996).

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Real Leather Wallet Mens Designer Leather WalletsandFirefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafetyshow the gel chromatographic separation of soluble protein with Sephadex G-Customized Hot Forging Agricultural Spare Parts.16mm 12 Strand UHMWPE Synthetic Fiber Rope, and another peak at 45 mL not shown inStainless Steel Cross Recesed Flat Head Self Tapping Screw, for peptides lower than 3000 Da.High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems(Condell et al.,1993) .

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Vertical Type Caseformer Machine WJ-LKX-15, V_e, and K_avcalculated as discussed previously.Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine,Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Stop Dog From Jumping or Climbing Your Chain Link Fence.) (Reis et al.,1989;ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment.,1991;Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel.,1991).0.1mm Tungsten Carbide 2f Single Flute Micro End Mill(1992), were lower, except for cystine and tyrosine,Agriculture Large Span Commerical Steel Frame Greenhouse.

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CONCLUSIONS

Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine) for the financial support.

REFERENCES

14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dewatering Screen Panels / Wedge Wire Panel]

Brown, E. M; Thompson, C .J.,Ss400 Corner Metal Sheet Bar Galvanized Angle Iron, M.M.(1994),High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Army Captain Navy Blue Color Hat for Army Tender]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer. Analytical Biochemistry212,436-445[Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor]

Dalev, P.G. and Simeonova, L.S.(1992),Chinese Jbcz Handy Operation Threading Machine and Coupler. Biotechnology letters 14,6, 531-534[Polyester Coated Stainless Steel Ladder Type Metal Ties]

Heidemann, E.(1997),Promotional Reusable Foldable PP Non Woven Shopping Bag.Leder 48,(5),99-104[2-Seater Waiting Chair]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Excavator Radiator /Oil Radiator /Water Radiator Manufacture]

Colombia Outdoor P5 Pantallas LED Display, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board.Factory Price Printing Doy Pack Bag. USP/SP[Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[380mm Working Area Concrete Diamond Floor Edge Grinder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High End Ultra High Pressure Water Jet Cutter for Hard Materials]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PRO-Installer Fiber Optic Termination Kit Lk-6016]

New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner]

New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Flat Surface Mounted 300mm 24W LED Panel Light for Interior]

New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17, Part I.Engine Parts Rubber High Quality Timing Belt 111ru25. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Warm Women Suede Leisure Snow Boots for Winter (FF-1)]

Wholesale Interior Metal 3D Bee Garden Ornament Decoration, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket.Disposable Non-Woven Dry Gloves Wipes Making Machine, 175-184[Industrial Ceramic Plate]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price]

Whitaker,J.R.(1963),ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting. Analytical Chemistry35(12), 1950-1953[Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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