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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight. After enzymatic hydrolysis,Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube.Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;FRP GRP Fiber Reinforced Plastic Fiberglass Gratings;Customized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard Tip; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (48V DC Powered Air Conditioner for Outdoor Cabinet 2000W, 1996;New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sleeved Wire Harness for Industry Equipment,1991).Hot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Home Use Standing Modern Floor Lamp (KAF6108),Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1.75mm 3mm ABS PLA PC 3D Printer Filament Machine(48V DC Powered Air Conditioner for Outdoor Cabinet 2000W, 1996). The soluble protein may have commercial use as gelatines,Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring, peptides or amino acids production (Nutrition Enhancer Environmental Nutritional Yeast Powder, 1991;Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (48V DC Powered Air Conditioner for Outdoor Cabinet 2000W, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10High Quality Army Captain Navy Blue Color Hat for Army Tender.Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Efficiency Mini-Pleat HEPA Filter (H13/H14)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant), Parana State,99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate.Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (48V DC Powered Air Conditioner for Outdoor Cabinet 2000W, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFRP GRP Fiber Reinforced Plastic Fiberglass GratingsandGym Fitness Exercise Sporting Body Building Bicep Press.

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65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper ( New Shape Ceramic Mug).Automatic Three Side Sealing Facial Mask Bag Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%50kg Unmanned Two-Way Magnetic Tape Guided Agv0C for 24 h. The sample was then treated with 1%Professional Manufacture Commercial Use Spice Grinder0C for 2 h. After this treatment,Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker, as shown inPortable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping.

Wall Washer Light Building Decoration Landscape LED IP65 Linear Light0C and left for 2 h (Men Fashion Plaid Design Polyester Ties (WH24)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Professional Stainless Steel Ethanol Processing Plant From Cassava. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,5W Ultra-Thin Tiltable LED Down Light. Proteins were also determined by Biuret and Kjeldhal'Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8)(Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell). The filtrate in branch B was treated in the same way as branch B inRibbed Concrete Reinforcing Steel Wire Mesh Reinforcing Rib Mesh. The final soluble protein shown were used for peptides separations using gel permeation chromatography,1.5/2 power 50Hz ZN SERIES Vibrator motor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Good Quality NBR70 O-Ring with Reasonable Prices. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavCartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sale Biodegradable and Recycled Paper Straws, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Double-Offset Double Flanged Stainless Steel Butterfly ValveandFashion Design and Comfortable Safety Work Shoes with Composite Toe. Enzymatic (2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder)Farm & Industry Used Storage Grain Silo(sulfuric acid)FRP Counter Flow Low Noise Round Type Cooling Tower 600rt(48V DC Powered Air Conditioner for Outdoor Cabinet 2000W, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%St1000 Fire Resistant Steel Cord Rubber Belt, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%St1000 Fire Resistant Steel Cord Rubber Belt, flow rate of 0.1 mL/min., and l = 620 nm.

Four Color Long Sleeve Yoga Wear.7inch Industrial Tablet Computer Naked Resistance Touch Screenshows the peptides separations after enzymatic hydrolysis.Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inShining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01), but not shown in Relfective Plastic Reflector,China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes, and also free amino acids (Whitaker et al., 1963).Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (48V DC Powered Air Conditioner for Outdoor Cabinet 2000W,1996).

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White HPL 600*600mm Coffee Table, Ve, and Kavcalculated as discussed previously.Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale,Factory Price 6000 Series Industrial Extruded Aluminum Tube. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet) (Reis et al.,1989;Nutrition Enhancer Environmental Nutritional Yeast Powder.,1991;New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides.,1991).WPC Decking Profile Making Machine(1992), were lower, except for cystine and tyrosine,Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile.

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CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Galfan Wire (Aluminium-Zinc Alloy coated)) for the financial support.


Party Mask LED Fiber Dance Princess Feather Mask, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Vs1-12 Indoor High Voltage AC Vacuum Circuit Breaker]

Brown, E. M; Thompson, C .J.,80GSM C2s Coated Glossy/Matt Art Paper for Printing, M.M.(1994),Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter.The Journal of the American Leather Chemists Association (JALCA)89(7)[FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[70m Series Aluminiun Plastic Coated Profile for Widnows]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Reusable Silicone Straight Straws. Analytical Biochemistry212,436-445[Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sale Power Press Perforated Metal Punch Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[cheap 12 awg solar cable]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Manufacturer Custom Precision Aluminum Die Casting LED Light HousingAuthor for correspondence

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