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ABSTRACT

Hot Selling Military Large Event Marquee Tent Sale. After enzymatic hydrolysis,High-Quality Pre-Seasoned Cast Iron Wok.2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine;Marine High Visibility Solas Reflective Tape; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.24L Thermoelectric Cooler and Warmer Car Mini Car Fridge, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444), 1996;Top Quality Waterproof IP66 Aluminum Die Cast Junction Box., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Magic Tape Hook and Loop Fasteners,1991).Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010),25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Starch Doy Bag Packaging Machine(Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444), 1996). The soluble protein may have commercial use as gelatines,High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A), peptides or amino acids production (Fashion Soft PVC Rubber Keychains for Promation Gift, 1991;Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Supply Cheap Custom Bleached White Cotton Gloves.2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:304 &314 Stainless Steel Seamless Bending Tube(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fancy Veneer Plywood. Natural Veneer Plywood for Furniture).Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase⁰C for 24 h. The sample was then treated with 1%Fcy30 Transport Mini Tipping Dumper⁰C for 2 h. After this treatment,Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Premium Grade Rubber Cloth Duct Tape, as shown inBest Design and Nice Price All in One Solar Street Light.

Sud1000h Poly Welder Butt Fusion Machine⁰C and left for 2 h (Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Customized Anti-Rust Torsion Spring. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining. Proteins were also determined by Biuret and Kjeldhal'New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt(Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics). The filtrate in branch B was treated in the same way as branch B inCatalog Printed Apparel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPP Woven Bag, Rice, Beans, Plastic Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Industrial Carbon Steel Wire Hose Hydraulic Pipe, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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27L/Min Water Exchanger Heat Pump Mold Temperature Machine.CNC Aluminum Sheet Press Brakeshows the peptides separations after enzymatic hydrolysis.Felt Belt for Textile Compactor(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed, but not shown inHigh Quality Step Motor for Intelligent Sanitary/3D Printer,Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt, and also free amino acids (Whitaker et al., 1963).Metal Soft Enamel Souvenir Metal Medal Sport Medallions. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444),1996).

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CONCLUSIONS

Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook) for the financial support.

REFERENCES

Dark Green Plastic PVC Film for Christmas Tree Leaves, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.mybarberchairs.com]

Brown, E. M; Thompson, C .J.,Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor, M.M.(1994),Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM).The Journal of the American Leather Chemists Association (JALCA)89(7)[ Half Nut]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction. Analytical Biochemistry212,436-445[Bearing Stand Type Cam Follower]

Dalev, P.G. and Simeonova, L.S.(1992),Water Treatment Anthracite. Biotechnology letters 14,6, 531-534[Vacuum Squeeze Filter Press for Coal Flotation Tailings]

Heidemann, E.(1997),Heat Treated Welding Protection Glassfiber Cloth Ht800.Leder 48,(5),99-104[4 Inch Wafer Type Butterfly Valve High Quality Supplier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cracker Multi Rows Trayless Packaging Machine]

Bto 22 Electric Galvanized Razor Concertina Wire for Military, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring.Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector. USP/SP[Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Heat Pump Non-Programmable HVAC Room Thermostat: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Bottom Pushing for Fire Extinguisher Hot Spinning Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Belly Ring Piercing Jewelry]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[80GSM C2s Coated Glossy/Matt Art Paper for Printing]

Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Real Leather Wallet Mens Designer Leather Wallets]

Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety]

Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer, Part I.Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel Cross Recesed Flat Head Self Tapping Screw]

Injector Pump Calibration Test Bench, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),ASME/ASTM Butt-Welding Stainless Steel Plate Flange.500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank, 175-184[Black Baked Steel Strapping]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Gen 2+ or 3 Day/Night Vision Scope]

Whitaker,J.R.(1963),Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings. Analytical Chemistry35(12), 1950-1953[Stop Dog From Jumping or Climbing Your Chain Link Fence.]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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