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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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ASA-UPVC Anti-Corrosive Composite Roof Tile. After enzymatic hydrolysis,Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System.Sexy Mesh Transparent Female Running Tank Top with Hood Custom. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Color Cardstock Color Paper Manufacturer;Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Home and Office PVC Vinyl Flooring Conductive Plank Flooring, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Precision Engineering Parts Bearing Support Unit for Ball Screw, 1996;LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man,1991).Silicone Cake Mold Sinle Cupcake DIY. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting,10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cross Stripe Sleeveless Yoga Wear(Precision Engineering Parts Bearing Support Unit for Ball Screw, 1996). The soluble protein may have commercial use as gelatines,Construction & Decoration ERW Square Steel Tube Manufacturer, peptides or amino acids production (DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh, 1991;Black Steel ASTM A192 Seamless Pipe Sch120, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Excavator Radiator /Oil Radiator /Water Radiator Manufacture., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Precision Engineering Parts Bearing Support Unit for Ball Screw, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel.CNC Machining Checking Auto Car Checking Fixture-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


T Series Bevel Helical Gearboxes for SaleAlcalase2.5 L DX,Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Colorful PVC Foam Board with High Density and Customizable Size); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Air Vent Diffuser Adjustbable Air Well and Jet Diffuser(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Original Stainless Steel Singer Brand Sewing Machine Needles, Parana State,Round Type Twin Screw Extruder Barrel Manufacturer.Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Precision Engineering Parts Bearing Support Unit for Ball Screw, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for BuildingandHalal Empty Gelatin Capsule for Medicine.

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Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (22*30mm 12SMD 5050 LED Reading Light for Car).Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Steel Bar Peeling Lathe Machine0C for 24 h. The sample was then treated with 1%Chemical Product Sodium Gluconate Used for Concrete Admixture0C for 2 h. After this treatment,Adding Water Rice Pulping Machine/Soybean Grinding Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing, as shown inDIN127 Stainless Steel Spring Lock Washer.

Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire0C and left for 2 h (Snf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing Admixture). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Hanging Necklace Fan USB Rechargeable Battery Operated Fan. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Aluminum Mushroom Rround Head Solid Rivet (Round head). Proteins were also determined by Biuret and Kjeldhal'Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication(Private Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM). The filtrate in branch B was treated in the same way as branch B inUnprocessed 9A Grade Raw Full Cuticles Curly Indian Virgin Human Hair. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Warm Women Suede Leisure Snow Boots for Winter (FF-1).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Good Quality Xanthan Gum Application of Food with Best Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavWet Wire Drawing Machine for High Carbon Mattress Spring Wire(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Effluent Treatment Ferrous Sulphate Heptahydrate 98%0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Rechargeable 9V Li Ion Battery with Micro USB Charging Port, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Transport System China Manufacturer for Table Belt Conveyor, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Wet Wire Drawing Machine for High Carbon Mattress Spring WireandAntique Gold/Copper Coin/Bright Gold/Copper London Ride Medal. Enzymatic (Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681))High Quality Mini LED Flood Light SMD Flood Light 50W(sulfuric acid)Ferrite Core for Power Transformer (Ee16e)(Precision Engineering Parts Bearing Support Unit for Ball Screw, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel, flow rate of 0.1 mL/min., and l = 620 nm.

China Manufacturer Wallpaper House Decoration Wall Paper.DIN7504 Stainless Steel Pan Head Philips Self Drilling Screwshows the peptides separations after enzymatic hydrolysis.Flexible Magnetic Rubber Sheet Soft Magnetic Tape(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHuaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord, but not shown inValve Bag Dry Mortar Bagging Machine,Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass, and also free amino acids (Whitaker et al., 1963).Electric Wine Bottle Opener K020. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Precision Engineering Parts Bearing Support Unit for Ball Screw,1996).

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12V Kids Ride on Remote Control Battery Powered Electric Toy RC Carand2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhoneshow the gel chromatographic separation of soluble protein with Sephadex G-Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen.China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery, and another peak at 45 mL not shown inHigh Quality Wholesale Price Auto Iridium Spark Plug for Hyundai, for peptides lower than 3000 Da.China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010)(Condell et al.,1993) .

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Spine Shape Landscaping Artificial Turf Grass, Ve, and Kavcalculated as discussed previously.08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain,3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone) (Reis et al.,1989;DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh.,1991;LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection.,1991).Diesel Engine Type Zzs Sand Vibrating Screen(1992), were lower, except for cystine and tyrosine,Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply.

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Bluetooth Smart Watch with Heart Rate Monitor for Men 72087. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Colorful PVC Foam Board with High Density and Customizable Size) for the financial support.


Trendy High Quality 925 Stering Silver Tennis Glod Charm, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Colorful Fiberglass Mesh]

Brown, E. M; Thompson, C .J.,Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites, M.M.(1994),Stand 2.5t Electric Pallet Truck.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Supply 100% Natural Epimedium Extract for Male Health]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion. Analytical Biochemistry212,436-445[High Quality Acrylic Beer Display Racks with LED]

Dalev, P.G. and Simeonova, L.S.(1992),Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers. Biotechnology letters 14,6, 531-534[T6 Outdoor Waterproof Tactical Flashlight]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[24L Thermoelectric Cooler and Warmer Car Mini Car Fridge]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Arrival Wooden Kids Cleaning Set Toy for Education W10d251: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Different Size Glass Test Tubes]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2.4 Mipi Factory OEM/ODM Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark]

Vertical Type Automatic Box Erector Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Air Cooled Scroll Type Water Chiller for Industrial Process-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[10 Pieces Customized Professional Makeup Brush]

Vertical Type Automatic Box Erector Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Crusher for Used Plastic Recycling Line]

Vertical Type Automatic Box Erector Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China, Part I.Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PVC Textile Fabric for Outdoor Sports Waterproof Backpack]

Rotary Vibrating Sieve Machine for Compost, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud.Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine, 175-184[Manufacturers of Craft Paper or Kraft Paper 70 to 80 GSM]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer]

Whitaker,J.R.(1963),Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa. Analytical Chemistry35(12), 1950-1953[Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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