ARTICLES

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ABSTRACT

Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump. After enzymatic hydrolysis,ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment.Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen;DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Auto Two Piece Folder Gluer for Corrugated Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (British Standard 2 Gang 1 Way Wall Light Switch, 1996;Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage,1991).Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier,Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails(British Standard 2 Gang 1 Way Wall Light Switch, 1996). The soluble protein may have commercial use as gelatines,Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire, peptides or amino acids production (Rectangular Stainless Steel Pipe SS304, 1991;High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;6.6kw LiFePO4 Charger for Lithium Battery Pack., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-50m Depth Long Range Metal Detector with Reliable Quality. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (British Standard 2 Gang 1 Way Wall Light Switch, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040).IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Customized Stable Burn-in Test Equipment(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Six Stations Pen Press Machine, Parana State,Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet.FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (British Standard 2 Gang 1 Way Wall Light Switch, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theEPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Priceand Metal Window Screen.

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Vertical Automatic Granule Bag Packing Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine).Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures⁰C for 24 h. The sample was then treated with 1%4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr⁰C for 2 h. After this treatment,Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Small Manufacturing Tht/ Ai Double Wave Soldering Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Manual Sealing Machine for Instant Noodles Paper Soup Bowls, as shown inFood Grade Stainless Steel Milk Dairy Equipment for Factory.

Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck⁰C and left for 2 h (High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion. Proteins were also determined by Biuret and Kjeldhal'Corrugated Steel Sheet Corrugated Steel Roofing Sheet(Chinese Jbcz Handy Operation Threading Machine and Coupler). The filtrate in branch B was treated in the same way as branch B inDistribution Coil Core. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Factory Queen Mattress with Visco Memory Foam and Latex.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBaijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.16mm 12 Strand UHMWPE Synthetic Fiber Rope⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Vertical Type Caseformer Machine WJ-LKX-15, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Fashion Design and Comfortable Safety Work Shoes with Composite ToeandFb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine. Enzymatic (Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould)0.1mm Tungsten Carbide 2f Single Flute Micro End Mill(sulfuric acid)Agriculture Large Span Commerical Steel Frame Greenhouse(British Standard 2 Gang 1 Way Wall Light Switch, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat, flow rate of 0.1 mL/min., and l = 620 nm.

14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags.Hot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machineshows the peptides separations after enzymatic hydrolysis.Ss400 Corner Metal Sheet Bar Galvanized Angle Iron(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDirect Factory Tebuconazole Price Fao Bactericide Tebuconazole Price, but not shown inChina High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter,High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine, and also free amino acids (Whitaker et al., 1963).Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (British Standard 2 Gang 1 Way Wall Light Switch,1996).

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Factory Price Printing Doy Pack Bag, V_e, and K_avcalculated as discussed previously.Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment,New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Chicken Layer Cage Poultry Cage Chicken Cages) (Reis et al.,1989;Rectangular Stainless Steel Pipe SS304.,1991;Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts.,1991).Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price(1992), were lower, except for cystine and tyrosine,High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17.

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CONCLUSIONS

Engine Parts Rubber High Quality Timing Belt 111ru25. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Promotional Customized PP Hand Fan, Plastic Folding Fan) for the financial support.

REFERENCES

Wholesale Interior Metal 3D Bee Garden Ornament Decoration, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality and Efficient Combustion UHP Graphite Electrode]

Brown, E. M; Thompson, C .J.,Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket, M.M.(1994),Disposable Non-Woven Dry Gloves Wipes Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sleeved Wire Harness for Industry Equipment]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Promotional Reusable Foldable PP Non Woven Shopping Bag]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting. Analytical Biochemistry212,436-445[PVC Cross Joint Pipe Fittings Equal Pipe Cross]

Dalev, P.G. and Simeonova, L.S.(1992),Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65. Biotechnology letters 14,6, 531-534[Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries]

Heidemann, E.(1997),Bottom Pushing for Fire Extinguisher Hot Spinning Machine.Leder 48,(5),99-104[Anodized Aluminum Profile]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers]

Hot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Fish Scales Texture Fashion Leather for Shoe (S312100GH).Diamond Segments 2000mm Diameter Granite Cutting Blade. USP/SP[8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Modern American Style Beech Solid Wood Kitchen Cabinet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Produce N2o for Medical Gas Equipments: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[8mm Heavy Duty Nails U Shaped Galvanized Staples]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[800W Pipe Welding Socket Fusion Machine Plastic Welder 63mm]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower]

Hot Selling Furring Channel Fittings Suspended Ceiling Accessories, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),90m3 Per Hour Mobile Concrete Pump-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Supermarket Plastic T-Wire Basket Swing up Price Label Holder]

Hot Selling Furring Channel Fittings Suspended Ceiling Accessories,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Small Frame Hydraulic Press Machine for Bending Price]

Hot Selling Furring Channel Fittings Suspended Ceiling Accessories, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter, Part I.FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Familu Use Mini Small Pig Feed Pellet Mill]

All Weather Rattan Handwoven Swing Outdoor Home, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pond Self Priming Booster suction  Water Pump with Pressure Switch.Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter, 175-184[China Mdi Prepolymer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cherry Wooden Grain PVC Film in Furniture]

Whitaker,J.R.(1963),Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace. Analytical Chemistry35(12), 1950-1953[High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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