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ABSTRACT

Industry 0.002mm Accuracy Checking Fixture. After enzymatic hydrolysis,Promotional Customized PP Hand Fan, Plastic Folding Fan.450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Vertical Automatic Granule Bag Packing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil, 1996;160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr,1991).Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Small Manufacturing Tht/ Ai Double Wave Soldering Machine,Manual Sealing Machine for Instant Noodles Paper Soup Bowls,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck(Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil, 1996). The soluble protein may have commercial use as gelatines,Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading, peptides or amino acids production (190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion, 1991;Corrugated Steel Sheet Corrugated Steel Roofing Sheet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Factory Queen Mattress with Visco Memory Foam and Latex., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Customized Hot Forging Agricultural Spare Parts.16mm 12 Strand UHMWPE Synthetic Fiber Rope-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient).Ss400 Corner Metal Sheet Bar Galvanized Angle Iron(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine⁰C for 24 h. The sample was then treated with 1%Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer⁰C for 2 h. After this treatment,Chinese Jbcz Handy Operation Threading Machine and Coupler. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Promotional Reusable Foldable PP Non Woven Shopping Bag's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board, as shown inLithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Engine Parts Rubber High Quality Timing Belt 111ru25. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Interior Metal 3D Bee Garden Ornament Decoration(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Disposable Non-Woven Dry Gloves Wipes Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile, V_e, and K_avcalculated as discussed previously.100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree,School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage) (Reis et al.,1989;190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion.,1991;160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures.,1991).Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle(1992), were lower, except for cystine and tyrosine,Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation.

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CONCLUSIONS

0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould) for the financial support.

REFERENCES

125kHz RFID Hotel Key Card with T5577 Chip, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Animal Manure Compost Organic Fertilizer Granular Machine with Ce]

Brown, E. M; Thompson, C .J.,USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug, M.M.(1994),Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Logo Fleece Hoodies Men Pullover Neno Oversized Men′s Hoodies]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Crop Fertilizer Agriculture Nh4cl Ammonium Chloride. Analytical Biochemistry212,436-445[27L/Min Water Exchanger Heat Pump Mold Temperature Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale High Pressure 80L Nitrogen Gas Cylinder. Biotechnology letters 14,6, 531-534[Custom All Kinds of Acrylic Shoe Display Racks]

Heidemann, E.(1997),10mm Red Green Yellow Warning China Supplier Signal Lamp.Leder 48,(5),99-104[Bedding Indoor and Outdoor Decorative]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Real Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for Watch]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Phillip Countersunk Head Screw Hot DIP Galvanized: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic OPP Slitter Cutting Machine (HX-320FQ vertical)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tf Sim Card Socket]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Medical Endoscope Disposable Bite Block with Bandage]

Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Disposable Non-Woven Dry Gloves Wipes Making Machine]

Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut]

Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Heat Shrink Automatic Flow Wrapping Packing Machine, Part I.Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Rotary Vibrating Sieve Machine for Compost]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sublimation Polyester Sports Tshirt for Adult]

Whitaker,J.R.(1963),5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building. Analytical Chemistry35(12), 1950-1953[CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Agriculture Large Span Commerical Steel Frame GreenhouseAuthor for correspondence