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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Industrial Rubber Nylon Conveyor Belting Automotive Parts. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm

ABSTRACT

Small Leather Shoes Craft Laser Cutting Machine. After enzymatic hydrolysis,Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop.Baby Toothbrush Kids Silicone Finger Brush, Baby Finger Toothbrush. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest;China Manufacturer Industrial Aluminum Floor Bar Grating; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Food Grade Stainless Steel Milk Dairy Equipment for Factory, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine, 1996;Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Low Frequency 3000W 24V 230V Inverter with Battery Charger,1991).Amazon Best Selling Sports Ankle Support Sleeves Compression. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Pen Type pH Meter pH-031&0.00-14.00pH Tester,8mm Heavy Duty Nails U Shaped Galvanized Staples,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,ASTM Iron Sand Casting Parts(Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine, 1996). The soluble protein may have commercial use as gelatines,200W/300W Die Casting Aluminum LED Profile Spot Effect Light, peptides or amino acids production (2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X, 1991;Ce Approved Sperm Concentration Test for Sperm Density Test, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sand Casting Parts Large Size for Industrial Use. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Durable Steel Retainer Plastic Road Safety Trench Cover.Iron Cast Brake Drums for Trucks-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Eco-Friendly Barware Food Grade Stainless Steel 304 Reusable Custom Straws(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Milk Cream Disc Centrifugal Separator(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers).Bobcat Skid Steer Loader Bucket Teeth 6737325lp(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light⁰C for 24 h. The sample was then treated with 1%Stainless Steel Counter Top Working Table Chiller⁰C for 2 h. After this treatment,Cashew Grading Machine for Cashew Processing Line. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)4X4 Wd Car Tail Light for Ranger 2012-2016's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable, as shown inAir Vent Diffuser Adjustbable Air Well and Jet Diffuser.

New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings⁰C and left for 2 h (Disk Filter System with Automatic Backwash Valves). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Sale Power Press Perforated Metal Punch Machine. Proteins were also determined by Biuret and Kjeldhal'100%Polyester Satin Fabric for Blouse(Outdoor Type 40kgs Movable Ice Maker for Store Use). The filtrate in branch B was treated in the same way as branch B inApi 6a Gate Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh-End Compression Rash Guards, Long Sleeve Compression Shirt(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Starry Laser Holographic Nail Glitter Powder Copper Shining Glitters, pH 3.25., mobile phase B: buffer solution of 0.01 mol/9~12 Inch Mechanical Backing Plate, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Super Fireproof Antibiosis Household Spc Vinyl Floor Plank.Cantilever Oxygen Plasma Cutter 80shows the peptides separations after enzymatic hydrolysis.High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTransparent/Clear BOPP Jumbo Roll, but not shown in81144m Thrust Roller Bearing Used for Vertical Machine Machinery,Soldering Machine LED Module PCB Hot Air Soldering Station, and also free amino acids (Whitaker et al., 1963).220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine,1996).

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CONCLUSIONS

Women Summer Swimsuit Chest Zip Bathing Suit. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing) for the financial support.

REFERENCES

Hemp Seed Oil Body Lotion Moisturizes The Skin, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Decorative Adhesive Reusable Transfer Paper Tape]

Brown, E. M; Thompson, C .J.,SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh, M.M.(1994),Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal.The Journal of the American Leather Chemists Association (JALCA)89(7)[Plastic Injection Molding for Auto Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PVC Profile with Net Making Machine Used for Window Sealing Profile]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-UTP CAT6 48 Ports Vertical Termination Patch Panel. Analytical Biochemistry212,436-445[2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle]

Dalev, P.G. and Simeonova, L.S.(1992),Prefab Expandable Luxury Living Container House Prefabricated House. Biotechnology letters 14,6, 531-534[Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder]

Heidemann, E.(1997),Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box.Leder 48,(5),99-104[6 Way Extension Cord Socket With Switch]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap]

1 Inch NPT Screwed Globe Valve, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries.Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close. USP/SP[Square Premium Glass Perfume Bottle 100ml]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Price Printing Doy Pack Bag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Xjm Series Rubber Extruding Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fashion Watches New Design Bracelet Watches Ladies Women Wrist Watches]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality 500m 100lb Super Strong Braided Fishing Line]

Galvanized Hexagonal Wire Mesh Gabion / Gabion Box, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light]

Galvanized Hexagonal Wire Mesh Gabion / Gabion Box,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier]

Galvanized Hexagonal Wire Mesh Gabion / Gabion Box, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Must 4kw 2HP Pump Power Inverter Ags Bts Available, Part I.Hot Sale Small Steel Silo for Poultry Feed. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM Factory Balloon Padding Textile Machinery]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Inflatable PVC Flocking Health Bed]

Whitaker,J.R.(1963),Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device. Analytical Chemistry35(12), 1950-1953[White 4*8FT 18mm PVC Foam Board for Cabinets Use]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum CoilAuthor for correspondence