ARTICLES

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ABSTRACT

Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds. After enzymatic hydrolysis,Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed.Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing;High Quality Inflatable PVC Flocking Health Bed; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cast Iron Butterfly Valve with Handles, 1996;Furniture Grade E1 Glue Plywood with Competitive Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer,1991).Underground Garden 6W High Power Outdoor LED Lawn Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stamping Metal Parts Manufacturer Sheet Parts Fabrication,Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY(Cast Iron Butterfly Valve with Handles, 1996). The soluble protein may have commercial use as gelatines,Hexagonal Netting/ Gabion Box/ Gabion Basket, peptides or amino acids production (Different Thickness High Quality Aluminum Foil for Glass Bottle Lid, 1991;Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Square Plastic PP Geocomposite Drainage Blind Ditch/Drain. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cast Iron Butterfly Valve with Handles, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Ozone Disinfection Machine Ozone Generator.Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PVC Profile with Net Making Machine Used for Window Sealing Profile(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Diameter 2.68m Paragraphed Autoclave for AAC Bricks, Parana State,Square Premium Glass Perfume Bottle 100ml.Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Cast Iron Butterfly Valve with Handles, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWater Based Emulsion Paint Production LineandSarm Steroids Ostarine / Mk-2866 / Mk2866 Powder for Bodybuilding CAS 841205-47-8.

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Travelling Weekender Mens Canvas Duffle Gym Sports Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Supplier Building Construction Steel Frame (ZY431)).4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC)⁰C for 24 h. The sample was then treated with 1%Best Selling Household Living Room Mat Chenille Carpets⁰C for 2 h. After this treatment,130HP 4WD Large Farm Power Engine Tractor From High Quality Factory. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Large Size Outdoor Store Front Signs Advertising Billboard, as shown inSc-2400 Automatic Multi Function Sosn Side Drilling Machine.

Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg⁰C and left for 2 h (Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1325 CNC Engraving Router. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel. Proteins were also determined by Biuret and Kjeldhal'T6s 86 PCD Core Bit for Geotechnical Drilling(IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump). The filtrate in branch B was treated in the same way as branch B inChina Heat Insulation Powder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CNC Machining 6601 Aluminum Parts with Anodized Automoblile. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCummins L10 Engine Piston Cooling Nozzle 3070218(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wvrb Series Planetary Gearbox Used for Robot⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/NPK 15 15 15 Compound Fertilizer at a Hot Selling, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Mini Blown Film Extrusion, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light.Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Lightshows the peptides separations after enzymatic hydrolysis.FSTN Type Monochrome Graphic Display Cog LCD with Backlight(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection, but not shown inLinen Quiltcover Set with Quilting,3D Embossed Iron on Label Clothing Heat Transfer Label, and also free amino acids (Whitaker et al., 1963).Glass Food Jar & Plastic Lid. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Cast Iron Butterfly Valve with Handles,1996).

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Sand Casting Parts Large Size for Industrial UseandAuto Two Piece Folder Gluer for Corrugated Boxshow the gel chromatographic separation of soluble protein with Sephadex G-0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer.Easy Operation Low Investment Cassava Silo, and another peak at 45 mL not shown inFactory Directly Selling Engine Standard Spark Plug, for peptides lower than 3000 Da.CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making(Condell et al.,1993) .

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100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve, V_e, and K_avcalculated as discussed previously.Building Material High Quality 316 Stainless Steel Grating,Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate) (Reis et al.,1989;Different Thickness High Quality Aluminum Foil for Glass Bottle Lid.,1991;Furniture Grade E1 Glue Plywood with Competitive Price.,1991).Sc-2400 Automatic Multi Function Sosn Side Drilling Machine(1992), were lower, except for cystine and tyrosine,Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair.

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CONCLUSIONS

Quality Shaper Machine Bc6085 Shapping Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line) for the financial support.

REFERENCES

Thermoplastic Road Marking Paint Glass Beads, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Direct Lit Back 35 Mm Led Flat Panel]

Brown, E. M; Thompson, C .J.,Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine, M.M.(1994),Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cnm Single Strand Anchor Coupler]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case. Analytical Biochemistry212,436-445[Fashion Design Underwear for Men Cotton Mens Boxer Briefs]

Dalev, P.G. and Simeonova, L.S.(1992),Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5. Biotechnology letters 14,6, 531-534[Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel]

Heidemann, E.(1997),Free Sample! AC Power Contactor for Sale.Leder 48,(5),99-104[Floor Sanding Block]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[IEC 62196-2 Type 2 EV Charging 32A Male Plug]

Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),PA System Loudspeaker 100W Outdoor Column Speaker .Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel. USP/SP[10mm Red Green Yellow Warning China Supplier Signal Lamp]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Durable Steel Retainer Plastic Road Safety Trench Cover]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Breathable Surgical Disposable 3 Ply Earloop Face Mask: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Products Genuine Leather En345-1 Steel Toe Cap Leather Safety Boots]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wear Resitant Rope End Cap Plastic Flange Cover]

Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CE Approved Manual Heat Press Machine]

Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign]

Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine, Part I.Flower Design Custom Made Silk Scarf. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[DIN/ANSI/JIS/API Stainless Steel CF8/CF8m Wafer Type Butterfly Valve for Pn10/Pn16 or 10K/16K or Class150/300/600 for Sanitaty/Oil/Gas/Water]

New Design Indoor Artificial Mini Rockery Fountains, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane.Medical Endoscope Disposable Bite Block with Bandage, 175-184[Hydrographic Survey System]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles]

Whitaker,J.R.(1963),Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels. Analytical Chemistry35(12), 1950-1953[2016 New Generation Sausage Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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