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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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Marine High Visibility Solas Reflective Tape. After enzymatic hydrolysis,24L Thermoelectric Cooler and Warmer Car Mini Car Fridge.Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Top Quality Waterproof IP66 Aluminum Die Cast Junction Box;Magic Tape Hook and Loop Fasteners; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010), 1996;25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Starch Doy Bag Packaging Machine,1991).High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fashion Soft PVC Rubber Keychains for Promation Gift,Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price(Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010), 1996). The soluble protein may have commercial use as gelatines,Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes, peptides or amino acids production (Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments, 1991;Factory Supply Cheap Custom Bleached White Cotton Gloves, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.304 &314 Stainless Steel Seamless Bending Tube.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Custom Grey Full-Zip Cotton Mens Hoodies.Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:7inch Industrial Tablet Computer Naked Resistance Touch Screen(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


Glass Door Passenger Sightseeing Elevator for SaleAlcalase2.5 L DX,Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase, Parana State,Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine.Fcy30 Transport Mini Tipping Dumper, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSide Pack Manual Gas Engine Power Brush CutterandStationery PP Clipboard With 7 Inch Scale Ruler.

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Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Day and Night Zebra Blind Fabric Z1602).Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Premium Grade Rubber Cloth Duct Tape0C for 24 h. The sample was then treated with 1%Sud1000h Poly Welder Butt Fusion Machine0C for 2 h. After this treatment,Customized Anti-Rust Torsion Spring. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, as shown inCustomized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter.

Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-600C and left for 2 h (Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,PP Woven Bag, Rice, Beans, Plastic Bag. Proteins were also determined by Biuret and Kjeldhal'China Professional Mold Factory Plastic Injection Mould Maker(Effluent Treatment Ferrous Sulphate Heptahydrate 98%). The filtrate in branch B was treated in the same way as branch B inDrum Chip for Konica Minolta C7450/C7450/7450. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavIndustrial Carbon Steel Wire Hose Hydraulic Pipe(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Clay Tiles Making Machine with Latest Technology0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/DIN Standard Gratings/Gavlanized Steel Grating, pH 3.25., mobile phase B: buffer solution of 0.01 mol/50mm Magnetic River Water Sewage Flow Meter for Acid, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Vacuum Stainless Steel Casting ProductsandPE Biodegradable Sheet Product Line/Single Screw Extruder. Enzymatic (27L/Min Water Exchanger Heat Pump Mold Temperature Machine)Felt Belt for Textile Compactor(sulfuric acid)Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt(Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Metal Soft Enamel Souvenir Metal Medal Sport Medallions, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Metal Soft Enamel Souvenir Metal Medal Sport Medallions, flow rate of 0.1 mL/min., and l = 620 nm.

20L Water Tank Lid Mold 3D Printing Samples Available.Low Volume Large Variety Non Standard Customized High Precision CNC Machining Partsshows the peptides separations after enzymatic hydrolysis.Wholesale High Quality Metal Car Mount Holder Phone Holder(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGuided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam, but not shown in Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building,Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch, and also free amino acids (Whitaker et al., 1963).High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010),1996).

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20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in ChinaandHemp Seed Oil Body Lotion Moisturizes The Skinshow the gel chromatographic separation of soluble protein with Sephadex G-Pet Laminate Aluminium Foil/Pet+Alu+PE.6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale, and another peak at 45 mL not shown in10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor, for peptides lower than 3000 Da.New Design Multi-Color Silicone Coin Purse for Lady(Condell et al.,1993) .

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Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065, Ve, and Kavcalculated as discussed previously.Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen,Dark Green Plastic PVC Film for Christmas Tree Leaves. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors ( Aerospace Equipment) (Reis et al.,1989;Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments.,1991;25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor.,1991).Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor(1992), were lower, except for cystine and tyrosine,Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM).

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1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor) for the financial support.


Water Treatment Anthracite, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Low Noise Wood Wool Machine Automatic Wood Shaving Machine]

Brown, E. M; Thompson, C .J.,Heat Treated Welding Protection Glassfiber Cloth Ht800, M.M.(1994),Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring.The Journal of the American Leather Chemists Association (JALCA)89(7)[Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ LED Bayomet Vehicle Lamps]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector. Analytical Biochemistry212,436-445[Phillip Countersunk Head Screw Hot DIP Galvanized]

Dalev, P.G. and Simeonova, L.S.(1992),Heat Pump Non-Programmable HVAC Room Thermostat. Biotechnology letters 14,6, 531-534[Automatic CNC Glass Cutting Machine Glass Cutting Table]

Heidemann, E.(1997),Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo.Leder 48,(5),99-104[Adult Bmx Bicycle]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook]

High-Quality Pre-Seasoned Cast Iron Wok, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable.Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer. USP/SP[Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[18X16mesh Fiberglass Window Screen Insect Screen]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Portable Metal Laser Cutting Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap]

Injector Pump Calibration Test Bench, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),ASME/ASTM Butt-Welding Stainless Steel Plate Flange-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[FRP Counter Flow Low Noise Round Type Cooling Tower 600rt]

Injector Pump Calibration Test Bench,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pipe Conveyor Belt]

Injector Pump Calibration Test Bench, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank, Part I.Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hydraulic Orbital Valve Power Steering]

Polyester / Polypropylene Woven Geotextile for Road Construction, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Granite Stone Jaw Crusher of Mining Machine.Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor, 175-184[Lost Wax Casting Machining Auto Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant]

Whitaker,J.R.(1963),Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer. Analytical Chemistry35(12), 1950-1953[Customized Large Ss Stamping/ Bending Sheet Metal Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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