ARTICLES

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ABSTRACT

Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll. After enzymatic hydrolysis,Heat Shrink Automatic Flow Wrapping Packing Machine.Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building, 1996;High Quality Sheet Metal Bending Roll Forming Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Different Size Glass Test Tubes, High Quality Laboratory Test Tubes,1991).Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Frame Type Rubber Hydraulic Press Machine,Kitchen Metal Dish Storage Rack (JP-SC984C),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming(5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building, 1996). The soluble protein may have commercial use as gelatines,AVR Se350 Automatic Voltage Regulator for Marathon Alternator, peptides or amino acids production (Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage, 1991;Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Strength Rigid PVC Plastic Foam Board for FRP., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve.Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cnm Single Strand Anchor Coupler⁰C and left for 2 h (Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,18X16mesh Fiberglass Window Screen Insect Screen. Proteins were also determined by Biuret and Kjeldhal'Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch(Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector). The filtrate in branch B was treated in the same way as branch B inSnack Chips Packaging Pouch. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Sterile Dsiposable Syringe for Dental 12ml.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Foldable Bed Military Cot for Camping. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avP5 960*960mm LED Display Screen SMD Indoor /Outdoor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Side Pack Manual Gas Engine Power Brush Cutter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Punching Bending Welding Steel Work Fabrication, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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IEC 62196-2 Type 2 EV Charging 32A Male Plug.Felt Belt for Textile Compactorshows the peptides separations after enzymatic hydrolysis.PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve, but not shown inChina Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery,180W 1850mm*80mm Reci Laser Tube, and also free amino acids (Whitaker et al., 1963).OEM Factory Balloon Padding Textile Machinery. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building,1996).

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CONCLUSIONS

Outdoor Cheap Portable Stage Aluminum Truss Stage. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module) for the financial support.

REFERENCES

One Arm Motorized Multifunction Hospital Equipment for China Supply, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[1.8 Mm Hot Dipped Galvanized Iron Wire]

Brown, E. M; Thompson, C .J.,Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts, M.M.(1994),P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Design Indoor Artificial Mini Rockery Fountains]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Galvanized Square Washer with Timber Constructions]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine. Analytical Biochemistry212,436-445[Food Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings. Biotechnology letters 14,6, 531-534[Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China]

Heidemann, E.(1997),Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery.Leder 48,(5),99-104[m.yishanjiushe.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ss400 Corner Metal Sheet Bar Galvanized Angle Iron]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Dolphin Designed Handheld Massager (LC-889): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Type Price Prefabricated Roof Tile in Sudan]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Electrical Box Latch Swinghandle Lock (MC4010B)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor]

Real Leather Wallet Mens Designer Leather Wallets, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),ASTM A240 321 Stainless Steel Sheet Price Per Kg-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9]

Real Leather Wallet Mens Designer Leather Wallets,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Aluminium Windows Thermal Break China Supplier]

Real Leather Wallet Mens Designer Leather Wallets, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor, Part I.Promotional Plain Silver Empty Hinged Rectangular Tin Box. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Super Fireproof Antibiosis Household Spc Vinyl Floor Plank]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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