Cummins L10 Engine Piston Cooling Nozzle 3070218

SciELO - Scientific Electronic Library Online

vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
Home Pagealphabetic serial listing

2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile

Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

Aluminium Machining Parts Turning Service Coffee Machine Milling Machine Spare Parts


Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor

Cow Horse Trailer Ramp Stall Stable Rubber MatEdivaldo E.GarciaI; Cláudia C.M.Hot Sale Thermostat Electric Heating Element for Solar Water HeatersI; Ariovaldo C.MartinsII; Gisele O. RochaII;Jorge Nozaki37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light

IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Phillip Countersunk Head Screw Hot DIP Galvanized. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse


Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll. After enzymatic hydrolysis,Heat Shrink Automatic Flow Wrapping Packing Machine.Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ductile Iron Standard Brackets Pipe Hanger;Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building, 1996;High Quality Sheet Metal Bending Roll Forming Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Different Size Glass Test Tubes, High Quality Laboratory Test Tubes,1991).Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Frame Type Rubber Hydraulic Press Machine,Kitchen Metal Dish Storage Rack (JP-SC984C),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming(5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building, 1996). The soluble protein may have commercial use as gelatines,AVR Se350 Automatic Voltage Regulator for Marathon Alternator, peptides or amino acids production (Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage, 1991;Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Strength Rigid PVC Plastic Foam Board for FRP., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve.Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 WheelsAlcalase2.5 L DX,Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)ADSS Newest Ultra Therapy Skin Tightening 3D Hifu(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap, Parana State,Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse.Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChildren Bear English Story Book Printingand KTV.

Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet

Smart Syringe Needle Automatic Retract Into Plunger

High Quality Ceramic Chrome Hybrid Bearing Oil(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder).Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder0C for 24 h. The sample was then treated with 1%Digger Arm Small Wheel Loader (HQ908) with CE0C for 2 h. After this treatment,15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Water Based Emulsion Paint Production Line, as shown inInvt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home.

Cnm Single Strand Anchor Coupler0C and left for 2 h (Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,18X16mesh Fiberglass Window Screen Insect Screen. Proteins were also determined by Biuret and Kjeldhal'Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch(Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector). The filtrate in branch B was treated in the same way as branch B inSnack Chips Packaging Pouch. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Sterile Dsiposable Syringe for Dental 12ml.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Foldable Bed Military Cot for Camping. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavP5 960*960mm LED Display Screen SMD Indoor /Outdoor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

2019 New Advertising Inflatables Air Walking Backpack Ball(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Side Pack Manual Gas Engine Power Brush Cutter0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Punching Bending Welding Steel Work Fabrication, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your LogoandAutomatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears. Enzymatic (1 Ton Chain Pulley Block with Geared Trolley)Powder Coating Polyester Resin with Factory Price(sulfuric acid)Auto Plastic Part Hardware Plastic Injection Mold(5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine, flow rate of 0.1 mL/min., and l = 620 nm.

IEC 62196-2 Type 2 EV Charging 32A Male Plug.Felt Belt for Textile Compactorshows the peptides separations after enzymatic hydrolysis.PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve, but not shown inChina Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery,180W 1850mm*80mm Reci Laser Tube, and also free amino acids (Whitaker et al., 1963).OEM Factory Balloon Padding Textile Machinery. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building,1996).

Vibratory Wheel Polishing Machine

Wall Washer Light Building Decoration Landscape LED IP65 Linear Light

marble Makeup Brush Set for Travel in 20PCSandRound Casting Iron Trench Cover Manhole Cover Frameshow the gel chromatographic separation of soluble protein with Sephadex G-NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy.Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089), and another peak at 45 mL not shown iniPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing, for peptides lower than 3000 Da.Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine(Condell et al.,1993) .

Independent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass Board

Outdoor Concert Stage Equipment Aluminum Stage Portable Stage, Ve, and Kavcalculated as discussed previously.Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home,22*30mm 12SMD 5050 LED Reading Light for Car. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Alu 2319 Slimline 12mm Wide Recessed Profile) (Reis et al.,1989;Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage.,1991;High Quality Sheet Metal Bending Roll Forming Machine.,1991).SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital(1992), were lower, except for cystine and tyrosine,Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness.

Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa

AAC Block Production Line / AAC Equipment Manufacturing/ Machinery


Outdoor Cheap Portable Stage Aluminum Truss Stage. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module) for the financial support.


One Arm Motorized Multifunction Hospital Equipment for China Supply, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[1.8 Mm Hot Dipped Galvanized Iron Wire]

Brown, E. M; Thompson, C .J.,Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts, M.M.(1994),P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Design Indoor Artificial Mini Rockery Fountains]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Galvanized Square Washer with Timber Constructions]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine. Analytical Biochemistry212,436-445[Food Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings. Biotechnology letters 14,6, 531-534[Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China]

Heidemann, E.(1997),Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery.Leder 48,(5),99-104[]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ss400 Corner Metal Sheet Bar Galvanized Angle Iron]

Hot Sale Thermostat Electric Heating Element for Solar Water Heaters, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Printed Wristband Cotton Wristband RFID Woven Wristband.High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles. USP/SP[Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Dolphin Designed Handheld Massager (LC-889): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Type Price Prefabricated Roof Tile in Sudan]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Electrical Box Latch Swinghandle Lock (MC4010B)]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor]

Real Leather Wallet Mens Designer Leather Wallets, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),ASTM A240 321 Stainless Steel Sheet Price Per Kg-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9]

Real Leather Wallet Mens Designer Leather Wallets,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Aluminium Windows Thermal Break China Supplier]

Real Leather Wallet Mens Designer Leather Wallets, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor, Part I.Promotional Plain Silver Empty Hinged Rectangular Tin Box. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker]

Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer.Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm), 175-184[Hyaluronic Acid Anti Aging Supplier & Manufacturer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Super Fireproof Antibiosis Household Spc Vinyl Floor Plank]

Whitaker,J.R.(1963),11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale. Analytical Chemistry35(12), 1950-1953[Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Aquarium Air Pump with 2 Nozzles Adjustable Gas FlowAuthor for correspondence

Creative Commons LicenseAll the contents of this journal, except where otherwise noted, is licensed under aCNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making