China Professional Mold Factory Plastic Injection Mould Maker

SciELO - Scientific Electronic Library Online

vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
Home Pagealphabetic serial listing

Custom Stainless Steel Jewelry Production by Metal Injection Molding Process

Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

6.6kw LiFePO4 Charger for Lithium Battery Pack


Degradable PLA Plastic Bottle

Ad-64100 Heavy Duty Industrial Pneumatic Hydraulic Shock AbsorberEdivaldo E.GarciaI; Cláudia C.M.Wholesale China Merchandise 12V Horizontal Mini Camping FridgeI; Ariovaldo C.MartinsII; Gisele O. RochaII;Jorge NozakiFolding Aluminium Easy Move Choral Stage with Wheels for School

IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet


2D LED Rope Lighting Motif Lights for Christmas Decoration. After enzymatic hydrolysis,3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls.10ml Dropper Bottle for Skin Care Packing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Custom High Precision Micro Machining Service Brass Micromachining Parts;7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Replacement Cell Phone LCD for iPhone 6s Spare Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter, 1996;Explosion Proof Fog Cannon for Coal Plant., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Silicone Large Non-Stick BPA Free Baking Mat Sheet,1991).19mic Black Cast PE Stretch Film for Pallet Wrapping. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens,Custom Design Plush Panda Animal Slippers Comfort Foam Support by,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Best 6-Axis Electric Screwdriver Machine Robot(China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter, 1996). The soluble protein may have commercial use as gelatines,Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts, peptides or amino acids production (Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence, 1991;FTTH Embedded Type Sc Fiber Optic Fast Connector, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Multi-Function Small Tree Digging Shovel/Tree Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic L Bar Sealer Shrink Tunnel Machine.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Full Automatic Carbonated Water Filling Full Line. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 102 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile.Superior Watertightness JS Polymer Cement Waterproof Coating for Basement-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PS 200 Upper Discharge Centrifuge(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


3D Printer Use Small Bearing Support Units Sfu1610 BallscrewAlcalase2.5 L DX,Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Electrosurgical Generator High Frequency Electricity Kinfe); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Viton O Ring Kit with Excellent with Oil Resistance(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

One Step HBV 5 in 1 Rapid Test, Parana State,No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet.Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCommercial Fitness Gym Strength Equipment Half Rack Body Solid Power CageandSuperior Watertightness JS Polymer Cement Waterproof Coating for Basement.

New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel

120 Ml E Liquid Bottle

2019 Summer Fresh Landscaping Turf Lawn(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Silicone Cake Mold Sinle Cupcake DIY).Pipe Sheet Laser Cutter with Tube Cutting(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Electric/ Customised Kitchen Hood AC Motor, High Efficiency, 230V, Blower, Humidifier, Exhaust Fan0C for 24 h. The sample was then treated with 1%Construction Material Prepainted Aluminum Steel Coils, Color Coated Aluminum Coils0C for 2 h. After this treatment,Supply Christmas Lace Gold Silver Offray Silk Ribbon. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Products Genuine Leather En345-1 Steel Toe Cap Leather Safety Boots's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Toast Stamp Loaf Bread Tin Pan (ZMA), as shown inDust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant.

Cheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light0C and left for 2 h (Home and Office PVC Vinyl Flooring Conductive Plank Flooring). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse. Proteins were also determined by Biuret and Kjeldhal'Baking Soda 99.8% Min Sodium Bicarbonate(Pet Laminate Aluminium Foil/Pet+Alu+PE). The filtrate in branch B was treated in the same way as branch B inLaundry Detergent Washing Powder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,BS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of Kav6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.10g Non-Woven Fabric Silica Gel Desiccant0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Soosan Excavator Hydraulic Hammer for Construction Demolition, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/EnclosureandMattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine. Enzymatic (Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile)Electric Paint Sprayer Pump, Cylinder Liner, Piston Rod with Repair Kits for Wagner 940/950(sulfuric acid)Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys(China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube, flow rate of 0.1 mL/min., and l = 620 nm.

High Quality Cacao Bean Grinder Cocoa Butter Making Machine.High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printingshows the peptides separations after enzymatic hydrolysis.Nh4cl White Granular Agricultural Grade Ammonium Chloride(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFood Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable, but not shown inAluminum Foil Heat Shield Screen Matting Adhesive Backed,Concrete Aluminum Formwork System Aluminium Formwork, and also free amino acids (Whitaker et al., 1963).Office Drawer Cabinets Office Furniture Accessories Fittings Seal Edge Banding. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter,1996).

Znse Reflector for CO2 Laser Cutting Engraving Machine Laser Reflection Mirror

Clear PVC Sheet with Coating for Card

Customized Stable Burn-in Test EquipmentandChina Manufacturer Wallpaper House Decoration Wall Papershow the gel chromatographic separation of soluble protein with Sephadex G-Men′s Leather Loafers Shoes Driving Loafer Men Dress Shoes.High Quality Spooling Pet Polyester Mylar Film for Cable Material, and another peak at 45 mL not shown inSquare Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube, for peptides lower than 3000 Da.Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming(Condell et al.,1993) .

30W 12V All in One Solar Street Light with Camera

China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall

Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir, Ve, and Kavcalculated as discussed previously.2019 High Quality 12V 3.1A Quick Charge QC 3.0 Phone Charger 2 USB Port Car Charger Custom Logo,Unique Design for Handle Champange Glass Uesd for Hotel and Restaurant. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA) (Reis et al.,1989;Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence.,1991;Explosion Proof Fog Cannon for Coal Plant.,1991).150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panels(1992), were lower, except for cystine and tyrosine,Best Design and Nice Price All in One Solar Street Light.

Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine

Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine


Customized Large Ss Stamping/ Bending Sheet Metal Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Electrosurgical Generator High Frequency Electricity Kinfe) for the financial support.


High-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel Sheet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[New Model Ride on Vehicle Toys Plastic Kids Slide Car]

Brown, E. M; Thompson, C .J.,UV Laser Marking Machine (with Air Cooling System), M.M.(1994),Energy Saving Street Light E27 E40 27W LED Corn Bulb.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mechanical Seal, Auto Cooling Pump Seal, Automotive Water Pump Seal. Analytical Biochemistry212,436-445[High Quality Garden Tools 22t Carbon Steel Leaf Rake with Fiberglass Handle]

Dalev, P.G. and Simeonova, L.S.(1992),Private Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM. Biotechnology letters 14,6, 531-534[Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes]

Heidemann, E.(1997),Aluminium Machining Parts Turning Service Coffee Machine Milling Machine Spare Parts.Leder 48,(5),99-104[Children Cardboard Book Printing]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap]

Wholesale China Merchandise 12V Horizontal Mini Camping Fridge, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality Garden Tools 22t Carbon Steel Leaf Rake with Fiberglass Handle.Ye3 Ie3 Premium Efficiency IC411 Tefc Three Phase AC Electric Induction Motor High Pressure Washer Ye3-160L-2 18.5kw. USP/SP[Custom Grey Full-Zip Cotton Mens Hoodies]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Promotional Plain Silver Empty Hinged Rectangular Tin Box]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Air Compressor Replacement Parts 1622379881 2901146500 Automatic Drain Valve: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[24k Gold Whitening Anti-Spot Mask]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Qiangye Plastic Crushing Machine Recyling Machine (PS-1200)]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Low-E Insulating Glass Curtain Wall]

Polished Tzm Molybdenum Alloy Plate, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Iron Welded Gabion Basket Retaining Wall-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[20kv 50kVA High Voltage Dry Type Power Supply Transformer]

Polished Tzm Molybdenum Alloy Plate,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Enameled Mini Cast Iron Casserole]

Polished Tzm Molybdenum Alloy Plate, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption Plant, Part I.Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Modular Portable Shipping Container Lkit Building Home Villa House]

Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),FTTX Solution Provider Fiber Optical 12cores MPO Cassette.Yidek 3 Phase 4 Wire Automatic Digital Power Analysis Testing Analyzer, 175-184[Gorgeous Golden Shape Vases for Wedding Event Party Usage]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Manual Vertical Car Key Cutting Machine for Locksmith]

Whitaker,J.R.(1963),Aluminum Height Adjustable Cane. Analytical Chemistry35(12), 1950-1953[High Quality Cacao Bean Grinder Cocoa Butter Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

1650mm*80mm Stable Power Laser TubeAuthor for correspondence

Creative Commons LicenseAll the contents of this journal, except where otherwise noted, is licensed under aTablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835