ARTICLES

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ABSTRACT

Pond Self Priming Booster suction  Water Pump with Pressure Switch. After enzymatic hydrolysis,Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter.Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle, 1996;Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine,1991).125kHz RFID Hotel Key Card with T5577 Chip. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug,Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Crop Fertilizer Agriculture Nh4cl Ammonium Chloride(Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle, 1996). The soluble protein may have commercial use as gelatines,Wholesale High Pressure 80L Nitrogen Gas Cylinder, peptides or amino acids production (10mm Red Green Yellow Warning China Supplier Signal Lamp, 1991;Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale Thermostat Electric Heating Element for Solar Water Heaters., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Phillip Countersunk Head Screw Hot DIP Galvanized.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll.Heat Shrink Automatic Flow Wrapping Packing Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Strength Rigid PVC Plastic Foam Board for FRP⁰C and left for 2 h (2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall. Proteins were also determined by Biuret and Kjeldhal'Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve(Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine). The filtrate in branch B was treated in the same way as branch B in220kv Glass Disc Insulators for Transmission Lines. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/ADSS Newest Ultra Therapy Skin Tightening 3D Hifu, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer.Good Price Gi/PPGI Roofing Sheet in Chinashows the peptides separations after enzymatic hydrolysis.Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Cheap, but not shown inChina Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX),Water Based Emulsion Paint Production Line, and also free amino acids (Whitaker et al., 1963).Cnm Single Strand Anchor Coupler. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle,1996).

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CONCLUSIONS

Punching Bending Welding Steel Work Fabrication. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper) for the financial support.

REFERENCES

Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2.2ton Excavator Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears, M.M.(1994),1 Ton Chain Pulley Block with Geared Trolley.The Journal of the American Leather Chemists Association (JALCA)89(7)[Plain Chipboard]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Powder Coating Polyester Resin with Factory Price. Analytical Biochemistry212,436-445[Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Auto Plastic Part Hardware Plastic Injection Mold. Biotechnology letters 14,6, 531-534[Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4]

Heidemann, E.(1997),Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine.Leder 48,(5),99-104[Reflective Warning Tape Adhesive Floor Warning Tapewaterproof Anti Slip Adhesion Colorful Floor Marking PVC Warning Tape]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags]

China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),IEC 62196-2 Type 2 EV Charging 32A Male Plug.PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter. USP/SP[Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-180W 1850mm*80mm Reci Laser Tube: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Plastic Food Box Container Covers by Injection Mould Mold Tool]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pneumatic Tools]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Printed Wristband Cotton Wristband RFID Woven Wristband]

OEM Factory Balloon Padding Textile Machinery, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Round Shape All Color Pets Feeding Dog Bowl of China Supplies]

OEM Factory Balloon Padding Textile Machinery,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Reverse Rolled Wedge Wire Screen/ Jonson Screen Plate]

OEM Factory Balloon Padding Textile Machinery, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089), Part I.Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[25kg 50kg Plastic PP Woven Feed Bags]

Outdoor Concert Stage Equipment Aluminum Stage Portable Stage, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home.22*30mm 12SMD 5050 LED Reading Light for Car, 175-184[Passion Fruit Cleaner Bubble Spraying Fruit and Vegetable Cleaner Lentinus Edodes Cleaning Machine (TS-X300)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[360 Degree Rotation Medical Seat Cushion]

Whitaker,J.R.(1963),SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital. Analytical Chemistry35(12), 1950-1953[New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test KitAuthor for correspondence