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ABSTRACT

Baby Feeding Chair Plastic Chair. After enzymatic hydrolysis,Reflective Hand Hold Thermoplastic Road Marking.Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge;Food Grade Stainless Steel Threated Butterfly Valve; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker, 1996;Suction Function Handheld Cool and Heat Food Bowl for Baby., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (9.0m Long 26PCS Globe Bulbs Solar Powered G40 LED String Light, for Patio Garden Backyard (Warm white),1991).JIS S Type Cast Iron Water Filter Dn25. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut,Factor Direct Adult Sex Toy Doll for Male Sex,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L(Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker, 1996). The soluble protein may have commercial use as gelatines,Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory, peptides or amino acids production (WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W, 1991;Worm Gear Fluorine Lined Butterfly Valve Flanged Type, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Poultry House, Livestock, Poultry Farm.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2.Black Slim PU Leather Passport Travel Wallet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with CeAlcalase2.5 L DX,3 Colors Tactical Shooting Hunting Camouflage Clothes Suit, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-1.3KG Fire breaker for kitchen); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Korean Integrated CTO Activated Carbon Filter for Water Treatment, Parana State,3.5-7.0g Ozone Generator Purification ---Commercial.Vacuum Squeeze Filter Press for Coal Flotation Tailings, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Supplier Building Construction Steel Frame (ZY431)andSc-2400 Automatic Multi Function Sosn Side Drilling Machine.

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Modular Portable Shipping Container Lkit Building Home Villa House(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump).New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Supplier of Excavator Spare Parts for Caterpillar Cat330⁰C for 24 h. The sample was then treated with 1%Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal⁰C for 2 h. After this treatment,Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wheel-Type-Carriage Precision Heavy Duty Linear Guide's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Double-Offset Double Flanged Stainless Steel Butterfly Valve, as shown inSurprise! Range Hood Free! ! ! Juice Cart.

Automotive Acrylic Wholesale Masking Tape Factory Price⁰C and left for 2 h (Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Home Decoration Candle Glass Holder for Table Decorative. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars. Proteins were also determined by Biuret and Kjeldhal'Wholesale Black Galvanized Head Stainless Steel Screw(LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection). The filtrate in branch B was treated in the same way as branch B inCounter-Clip Fluorine-Lined Butterfly Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOEM Logo Soft Silicone Reusable BPA Free Drinking Straws(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.4 Drawer Steel Office Vertical Filing Cabinet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Parts for Crane Outrigger Hydraulic Oil Cylinder, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Linen Quiltcover Set with QuiltingandBoned Polyester Woven Faux Suede Sofa Fabric. Enzymatic (Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making)20 Bottles Electric Wine Display Cooler(sulfuric acid)High-Efficient Htk Walnut Shell Filter for Water Purification(Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576), flow rate of 0.1 mL/min., and l = 620 nm.

Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100).Hexagonal Netting/ Gabion Box/ Gabion Basketshows the peptides separations after enzymatic hydrolysis.Popular European Style White Marble Coffee Tables for Dinnig Room(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSand Casting Parts Large Size for Industrial Use, but not shown inAuto Two Piece Folder Gluer for Corrugated Box,High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket, and also free amino acids (Whitaker et al., 1963).FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker,1996).

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I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Boxand99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediateshow the gel chromatographic separation of soluble protein with Sephadex G-Kwikstage Scaffolding System-Diagonal Brace.360 Rotating Automatic Touchless Car Wash Equipment with Drying System, and another peak at 45 mL not shown inWomen Summer Swimsuit Chest Zip Bathing Suit, for peptides lower than 3000 Da.48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner(Condell et al.,1993) .

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Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox, V_e, and K_avcalculated as discussed previously.IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump,Sell Rubber / EVA / PE/PVC Foam Board. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065) (Reis et al.,1989;WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W.,1991;Suction Function Handheld Cool and Heat Food Bowl for Baby.,1991).Sodium Gluconate 98% Min Industry Grade for Chemical Admixture(1992), were lower, except for cystine and tyrosine,Brake Disc.

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CONCLUSIONS

DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (1.3KG Fire breaker for kitchen) for the financial support.

REFERENCES

24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Factory Supplier Monofilament Polypropylene Fiber with Certificate FDA Ce]

Brown, E. M; Thompson, C .J.,MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote, M.M.(1994),Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[IEC 62196-2 Type 2 EV Charging 32A Male Plug]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-LED Screen Display Wireless Charging 10000mAh Power Bank. Analytical Biochemistry212,436-445[10mm Red Green Yellow Warning China Supplier Signal Lamp]

Dalev, P.G. and Simeonova, L.S.(1992),25kg 50kg Plastic PP Woven Feed Bags. Biotechnology letters 14,6, 531-534[Durable Steel Retainer Plastic Road Safety Trench Cover]

Heidemann, E.(1997),360 Degree Rotation Medical Seat Cushion.Leder 48,(5),99-104[China Plant Stand]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CE Approved Manual Heat Press Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Good Quality Extrusion Bulking Machine Parts Screw Elements]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[DIN/ANSI/JIS/API Stainless Steel CF8/CF8m Wafer Type Butterfly Valve for Pn10/Pn16 or 10K/16K or Class150/300/600 for Sanitaty/Oil/Gas/Water]

2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Alu 2319 Slimline 12mm Wide Recessed Profile-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles]

2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2016 New Generation Sausage Making Machine]

2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo, Part I.Custom Size Christmas Gift Personalized Watch Boxes for Men. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Four Color Long Sleeve Yoga Wear]

12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector.Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality, 175-184[Shr Ipl Hair Removal Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Electric Heating Mantle 5L]

Whitaker,J.R.(1963),New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115. Analytical Chemistry35(12), 1950-1953[Electric Wine Bottle Opener K020]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Black Slim PU Leather Passport Travel WalletAuthor for correspondence