ARTICLES

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ABSTRACT

Printed Wristband Cotton Wristband RFID Woven Wristband. After enzymatic hydrolysis,High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles.Dolphin Designed Handheld Massager (LC-889). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Real Leather Wallet Mens Designer Leather Wallets;ASTM A240 321 Stainless Steel Sheet Price Per Kg; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Promotional Plain Silver Empty Hinged Rectangular Tin Box, 1996;Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer,1991).Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale,Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cantilever Oxygen Plasma Cutter 80(Promotional Plain Silver Empty Hinged Rectangular Tin Box, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Expansion Anchor Bolt Steel Zinc Plated, peptides or amino acids production (CNC Fiber Laser Cutting Machine for Sheet and Tube, 1991;Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Promotional Plain Silver Empty Hinged Rectangular Tin Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump.10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

BS 5163 Ductile Iron Rising Stem Gate ValveAlcalase2.5 L DX,CNC Aluminum Sheet Press Brake, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-ID25mm Od86mm Standard Through Hole Slip Ring); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Bio Media, Parana State,Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery.Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Promotional Plain Silver Empty Hinged Rectangular Tin Box, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRebonded Foam MachinaandVitamin B1 HCl 98% Feed Grade.

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Platform Trolley with 150kg Capacity, 4 Castor Wheel(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Atf-805 Auto Transmission System Flushing Machine with CE/SGS).Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V⁰C for 24 h. The sample was then treated with 1%All Size Colored Tapping Hex Head Self Drill Screw Wood Screw⁰C for 2 h. After this treatment,Pre-Engineered Cage Broiler Poultry Farm Building. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Alumina Ball with High Quality's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PVC Textile Fabric for Outdoor Sports Waterproof Backpack, as shown inTumble Belt Type Shot Blasting Machine for Iron Castings Parts.

White 4*8FT 18mm PVC Foam Board for Cabinets Use⁰C and left for 2 h (New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants. Proteins were also determined by Biuret and Kjeldhal'Powerful Anti-Stress, Strong Flexibility Spoke Wire(Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair). The filtrate in branch B was treated in the same way as branch B inPeri System Formwork. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Modern American Style Beech Solid Wood Kitchen Cabinet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hero Brand Polypropylene Bags Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCNC Machining Metal/Aluminum/Brass Auto Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Chicken Layer Cage Poultry Cage Chicken Cages⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/316L Cold Rolled Stainless Steel Coil 316L Inox, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Multipurpose Wireless PA Amplifier Lectern PA Speaker, V_e, and K_avcalculated as discussed previously.3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads,Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China) (Reis et al.,1989;CNC Fiber Laser Cutting Machine for Sheet and Tube.,1991;Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling.,1991).High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157(1992), were lower, except for cystine and tyrosine,12ml Round Shape Empty Clear Nail Polish Glass Bottle.

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CONCLUSIONS

M4X10 Round Head Core Pulling Aluminum Nail. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (ID25mm Od86mm Standard Through Hole Slip Ring) for the financial support.

REFERENCES

Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Vitamin B1 HCl 98% Feed Grade]

Brown, E. M; Thompson, C .J.,Skytone 4 Channels 3500W Each DJ Power Amplifier, M.M.(1994),Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price.The Journal of the American Leather Chemists Association (JALCA)89(7)[Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels. Analytical Biochemistry212,436-445[Pond Self Priming Booster suction  Water Pump with Pressure Switch]

Dalev, P.G. and Simeonova, L.S.(1992),2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture. Biotechnology letters 14,6, 531-534[Flexible Magnetic Rubber Sheet Soft Magnetic Tape]

Heidemann, E.(1997),Imirootree 250ml Glass Jar with Lids for Cosmetic Cream.Leder 48,(5),99-104[Stainless Steel Spring Nut U Clip Nut M10]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine]

Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Double Wine Carrier Bag Gift Box Tote.(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts. USP/SP[Ep/Nn/EPP Industrial Rubber Conveyor Belt]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[100%Polyester Satin Fabric for Blouse]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Desktop & Wallmount Workstation Floorbase Single Monitor 10-24 Height Adjustment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Business Desk Lamp Products Made in China]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Lantern for Garden Decoration]

Flexible PVC Granules for Cable, Wire and Insulation, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Energy Saving Street Light E27 E40 27W LED Corn Bulb]

Flexible PVC Granules for Cable, Wire and Insulation,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Multi-Function Small Tree Digging Shovel/Tree Machine]

Flexible PVC Granules for Cable, Wire and Insulation, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line, Part I.Brass Pump Spare Parts for Circulating Pump. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Stainless Steel Double Small Torsion Spring]

Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi.Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011, 175-184[CNC Aluminium Anodized Quick Change Chain Sprocket Hub Carrier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[1 Ton Chain Pulley Block with Geared Trolley]

Whitaker,J.R.(1963),Lantern for Garden Decoration. Analytical Chemistry35(12), 1950-1953[High Quality Spooling Pet Polyester Mylar Film for Cable Material]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Micro-Combustion TubeAuthor for correspondence