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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera. After enzymatic hydrolysis,Poultry House, Livestock, Poultry Farm.1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2;Black Slim PU Leather Passport Travel Wallet; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce, 1996;3 Colors Tactical Shooting Hunting Camouflage Clothes Suit., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (1.3KG Fire breaker for kitchen,1991).Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Korean Integrated CTO Activated Carbon Filter for Water Treatment,Vacuum Squeeze Filter Press for Coal Flotation Tailings,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Modular Portable Shipping Container Lkit Building Home Villa House(Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce, 1996). The soluble protein may have commercial use as gelatines,New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel, peptides or amino acids production (China Supplier of Excavator Spare Parts for Caterpillar Cat330, 1991;Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wheel-Type-Carriage Precision Heavy Duty Linear Guide.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Double-Offset Double Flanged Stainless Steel Butterfly Valve. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Automotive Acrylic Wholesale Masking Tape Factory Price.Home Decoration Candle Glass Holder for Table Decorative-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

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12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Insecticidal Lamp with Solar Energy Frequent Vibration).Parts for Crane Outrigger Hydraulic Oil Cylinder(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Boned Polyester Woven Faux Suede Sofa Fabric0C for 24 h. The sample was then treated with 1%Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making0C for 2 h. After this treatment,20 Bottles Electric Wine Display Cooler. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High-Efficient Htk Walnut Shell Filter for Water Purification's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576), as shown inHight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml.

Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)0C and left for 2 h (Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Popular European Style White Marble Coffee Tables for Dinnig Room. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket. Proteins were also determined by Biuret and Kjeldhal'FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System(Sequins Butterfly Handmade Beads Embroidery Patch). The filtrate in branch B was treated in the same way as branch B inwalk boards. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Kwikstage Scaffolding System-Diagonal Brace.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%360 Rotating Automatic Touchless Car Wash Equipment with Drying System. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of Kav48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sell Rubber / EVA / PE/PVC Foam Board, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sodium Gluconate 98% Min Industry Grade for Chemical Admixture, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper FactoryandBrake Disc. Enzymatic (DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign)24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat(sulfuric acid)MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote(Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder, flow rate of 0.1 mL/min., and l = 620 nm.

LED Screen Display Wireless Charging 10000mAh Power Bank.50mm Magnetic River Water Sewage Flow Meter for Acidshows the peptides separations after enzymatic hydrolysis.25kg 50kg Plastic PP Woven Feed Bags(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A), but not shown inMobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen,360 Degree Rotation Medical Seat Cushion, and also free amino acids (Whitaker et al., 1963).Custom LCD Screen Video Box for Package. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce,1996).

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Alu 2319 Slimline 12mm Wide Recessed Profile, Ve, and Kavcalculated as discussed previously.High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo,Custom Size Christmas Gift Personalized Watch Boxes for Men. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010)) (Reis et al.,1989;China Supplier of Excavator Spare Parts for Caterpillar Cat330.,1991;3 Colors Tactical Shooting Hunting Camouflage Clothes Suit.,1991).12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope(1992), were lower, except for cystine and tyrosine,8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector.

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Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair) for the financial support.


New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Big Wheel Loaders Wholesaler Quotes & PriceList]

Brown, E. M; Thompson, C .J.,China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX), M.M.(1994),Stainless Steel Cross Recesed Flat Head Self Tapping Screw.The Journal of the American Leather Chemists Association (JALCA)89(7)[Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cow Dung Chicken Manure Organic Fertilizer Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Our Perforated Aluminum & Solid Panel for New Office Wall Cladding. Analytical Biochemistry212,436-445[4X4 Wd Car Tail Light for Ranger 2012-2016]

Dalev, P.G. and Simeonova, L.S.(1992),2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone. Biotechnology letters 14,6, 531-534[Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8)]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[P5 960*960mm LED Display Screen SMD Indoor /Outdoor]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel Sheet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hydraulic Orbital Valve Power Steering: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Good Price Nasal Paste Packaging Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ie3 Induction Three Phase Fan Motor]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office]

W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),AISI 410 Stainless Steel Galvanized Wire for Making Scourer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Teerte Square Flat Head Self Tapping Screw]

W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom Sheet Metal Stamping Bending Product Manufacturing Service]

W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CS1363 a Standard UK 3 Pin Power Plug, Part I.Food Chopper. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Water Chiller Air Cooled Chiller Industrial Chiller for Sale]

Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Prices of 20W 40W 60W Solar Street Lights.ISO 3821 3/16′′ Twin Line Welding Hose for USA Market, 175-184[925 silver set]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D)]

Whitaker,J.R.(1963),Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins. Analytical Chemistry35(12), 1950-1953[13.56 MHz Fudan FM11RF08 Chip Card]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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