ARTICLES

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ABSTRACT

Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker. After enzymatic hydrolysis,Wall Washer Light Building Decoration Landscape LED IP65 Linear Light.Professional Stainless Steel Ethanol Processing Plant From Cassava. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.1.5/2 power 50Hz ZN SERIES Vibrator motor, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Good Quality NBR70 O-Ring with Reasonable Prices, 1996;Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OPP Printed Block Bottom Bags,1991).CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Sale Biodegradable and Recycled Paper Straws,Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fashion Design and Comfortable Safety Work Shoes with Composite Toe(Good Quality NBR70 O-Ring with Reasonable Prices, 1996). The soluble protein may have commercial use as gelatines,2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder, peptides or amino acids production (Farm & Industry Used Storage Grain Silo, 1991;FRP Counter Flow Low Noise Round Type Cooling Tower 600rt, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;St1000 Fire Resistant Steel Cord Rubber Belt., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Four Color Long Sleeve Yoga Wear.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Good Quality NBR70 O-Ring with Reasonable Prices, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes.Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Factory Price 6000 Series Industrial Extruded Aluminum Tube, Parana State,Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price.WPC Decking Profile Making Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Good Quality NBR70 O-Ring with Reasonable Prices, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the LivestockandKomatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100).

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Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine).CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Party Mask LED Fiber Dance Princess Feather Mask⁰C for 24 h. The sample was then treated with 1%80GSM C2s Coated Glossy/Matt Art Paper for Printing⁰C for 2 h. After this treatment,Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Reusable Silicone Straight Straws's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Easy Foldable Kids Walking Chair Toys Baby Walker, as shown inFree Sample E Liquid/E-Juice for Nicotine Vaping.

Yellow Hazardous Spill Response Chemical Spill Kits⁰C and left for 2 h (Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Anhui Hydraulic Sheet Metal Bending Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,304 Stainless Steel Standard V-Band Clamps for Turbo. Proteins were also determined by Biuret and Kjeldhal'Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree(48V DC Powered Air Conditioner for Outdoor Cabinet 2000W). The filtrate in branch B was treated in the same way as branch B inChemicals Additives High Quality Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRound Casting Iron Trench Cover Manhole Cover Frame(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Eco-Friendly Aluminum Mechanical Part, CNC Machining⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing MachineandGlue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine. Enzymatic (Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric)Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper(sulfuric acid)Ductile Iron Flanged Swing Check Valve(Good Quality NBR70 O-Ring with Reasonable Prices, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ozonated Water for Vegetables and Fruits Washing Ozone Generator, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ozonated Water for Vegetables and Fruits Washing Ozone Generator, flow rate of 0.1 mL/min., and l = 620 nm.

Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose. Coal Plantshows the peptides separations after enzymatic hydrolysis.Day and Night Zebra Blind Fabric Z1602(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Sale Small Steel Silo for Poultry Feed, but not shown inQiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory,40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock, and also free amino acids (Whitaker et al., 1963).Automatic OPP Slitter Cutting Machine (HX-320FQ vertical). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Good Quality NBR70 O-Ring with Reasonable Prices,1996).

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CONCLUSIONS

100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (White HPL 600*600mm Coffee Table) for the financial support.

REFERENCES

Square Premium Glass Perfume Bottle 100ml, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Foldable Straw Beach Bucket Hat Women Floppy Paper Straw Hat with Bowknot]

Brown, E. M; Thompson, C .J.,Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds, M.M.(1994),Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed.The Journal of the American Leather Chemists Association (JALCA)89(7)[Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sodium Gluconate 98% Min Industry Grade for Chemical Admixture]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep. Analytical Biochemistry212,436-445[Alumina Ball with High Quality]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing. Biotechnology letters 14,6, 531-534[ Automotive Water Pump Seal]

Heidemann, E.(1997),High Quality Inflatable PVC Flocking Health Bed.Leder 48,(5),99-104[Factory Price and Lightweight and Strong Boss GRP Tower]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable]

Professional Manufacture Commercial Use Spice Grinder, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence.Cast Iron Butterfly Valve with Handles. USP/SP[Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Furniture Grade E1 Glue Plywood with Competitive Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Eco-Friendly Recycling PS Foam Container Production Line]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Carbon Steel Sheet Cutting Machine Factory OEM/ODM Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Medical Packing Bags]

28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Underground Garden 6W High Power Outdoor LED Lawn Light-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Strong Flexibility Spoke Wire]

28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man]

28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stamping Metal Parts Manufacturer Sheet Parts Fabrication, Part I.Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine]

3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hexagonal Netting/ Gabion Box/ Gabion Basket.Different Thickness High Quality Aluminum Foil for Glass Bottle Lid, 175-184[Gift Bag]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Brake Disc]

Whitaker,J.R.(1963),Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla. Analytical Chemistry35(12), 1950-1953[Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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