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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.FSTN Type Monochrome Graphic Display Cog LCD with Backlight, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3D Embossed Iron on Label Clothing Heat Transfer Label, 1996;Glass Food Jar & Plastic Lid., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer,1991).Easy Operation Low Investment Cassava Silo. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making,100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Building Material High Quality 316 Stainless Steel Grating(3D Embossed Iron on Label Clothing Heat Transfer Label, 1996). The soluble protein may have commercial use as gelatines,Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table, peptides or amino acids production (Sc-2400 Automatic Multi Function Sosn Side Drilling Machine, 1991;Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Quality Shaper Machine Bc6085 Shapping Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Thermoplastic Road Marking Paint Glass Beads.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3D Embossed Iron on Label Clothing Heat Transfer Label, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts.I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Design Plush Panda Animal Slippers Comfort Foam Support by).Flower Design Custom Made Silk Scarf(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Design Indoor Artificial Mini Rockery Fountains⁰C for 24 h. The sample was then treated with 1%7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane⁰C for 2 h. After this treatment,Medical Endoscope Disposable Bite Block with Bandage. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08), as shown inFabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3120/17/38 Boltable Crane Rail Clips. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel) for the financial support.

REFERENCES

High Speed Automatic Packing Machine (GK-650 BA), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Air Brake Hose Line For Truck Bus]

Brown, E. M; Thompson, C .J.,5000-5900MHz 4G Built-in Automotive Antenna, M.M.(1994),Glass Lined Reactor for Chemical Factory.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM Metal Stamping Stainless Steel Parts with CNC Machining. Analytical Biochemistry212,436-445[4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone]

Dalev, P.G. and Simeonova, L.S.(1992),Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper. Biotechnology letters 14,6, 531-534[Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China]

Heidemann, E.(1997),Supermarket Plastic T-Wire Basket Swing up Price Label Holder.Leder 48,(5),99-104[Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Imirootree 250ml Glass Jar with Lids for Cosmetic Cream]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Acrylic Beer Display Racks with LED]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[100% Silver Fiber Clothing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[T6 Outdoor Waterproof Tactical Flashlight]

Degradable PLA Plastic Bottle, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale China Merchandise 12V Horizontal Mini Camping Fridge-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[24L Thermoelectric Cooler and Warmer Car Mini Car Fridge]

Degradable PLA Plastic Bottle,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws]

Degradable PLA Plastic Bottle, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House, Part I.No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157]

2D LED Rope Lighting Motif Lights for Christmas Decoration, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls.10ml Dropper Bottle for Skin Care Packing, 175-184[Anti-Reflective Anti-Finger Touch Monitor Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Different Size Glass Test Tubes]

Whitaker,J.R.(1963),Custom High Precision Micro Machining Service Brass Micromachining Parts. Analytical Chemistry35(12), 1950-1953[Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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