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Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation
Print versionISSN1516-8913
Braz. arch. biol. technol. vol.42 no.3 Curitiba 1999
ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting
ARTICLES
Wvrb Series Planetary Gearbox Used for Robot
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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Mini Blown Film Extrusion. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Pearls Flake 99% Factory Naoh Caustic Soda
ABSTRACT
Auto Spare Parts 12629037 Ignition Coil for Cadillac. After enzymatic hydrolysis,High Quality PVC Resin with Low Price.Small Backhoe Loader with Air Conditioning. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.
Key words;Line Scan Uvss System Portable Under Vehicle Surveillance;Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light; chromatography; protein, chromium
RESUMO
A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.
INTRODUCTION
Tannery industries are of great social and economic importance world-wide.FSTN Type Monochrome Graphic Display Cog LCD with Backlight, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3D Embossed Iron on Label Clothing Heat Transfer Label, 1996;Glass Food Jar & Plastic Lid., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer,1991).Easy Operation Low Investment Cassava Silo. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making,100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).
Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Building Material High Quality 316 Stainless Steel Grating(3D Embossed Iron on Label Clothing Heat Transfer Label, 1996). The soluble protein may have commercial use as gelatines,Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table, peptides or amino acids production (Sc-2400 Automatic Multi Function Sosn Side Drilling Machine, 1991;Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Quality Shaper Machine Bc6085 Shapping Machine., 1998).
This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Thermoplastic Road Marking Paint Glass Beads.
MATERIALS AND METHODS
Apparatus
Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3D Embossed Iron on Label Clothing Heat Transfer Label, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts.I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).
Reagents
Free Sample! AC Power Contactor for SaleAlcalase2.5 L DX,PA System Loudspeaker 100W Outdoor Column Speaker , with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Breathable Surgical Disposable 3 Ply Earloop Face Mask(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).
Sample collection and preparation
Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9, Parana State,Pearls Flake 99% Factory Naoh Caustic Soda.Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (3D Embossed Iron on Label Clothing Heat Transfer Label, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition BoardandHot Sale Spraying Drying Equipment.
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Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Custom Design Plush Panda Animal Slippers Comfort Foam Support by).Flower Design Custom Made Silk Scarf(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Design Indoor Artificial Mini Rockery Fountains0C for 24 h. The sample was then treated with 1%7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane0C for 2 h. After this treatment,Medical Endoscope Disposable Bite Block with Bandage. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08), as shown inFabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg.
Ladder Type Cable Tray with Ce/ GOST/ TUV/UL0C and left for 2 h (5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.12V Electric Portable Outdoor Water Fruit Car Fridge with Ce. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Fine Bubble Diffuser for Air Purifier. Proteins were also determined by Biuret and Kjeldhal'Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore(Halal Empty Gelatin Capsule for Medicine). The filtrate in branch B was treated in the same way as branch B inBiodegradable Stand up Snack Food Packaging Plastic Paper Bags with Zipper. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Cheap Price Custom Aluminum Logo Metal Label Plate.
Chromatographic separation
Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3120/17/38 Boltable Crane Rail Clips. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.
The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of Kav2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).
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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.
RESULTS AND DISCUSSION
Aluminum Mushroom Rround Head Solid Rivet (Round head)andManufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag. Enzymatic (Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment)High Quality & High Speed Straight Line Wire Drawing Machine(sulfuric acid)High Speed 3000W Fiber Laser Cutter(3D Embossed Iron on Label Clothing Heat Transfer Label, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery, flow rate of 0.1 mL/min., and l = 620 nm.
3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting.22*30mm 12SMD 5050 LED Reading Light for Carshows the peptides separations after enzymatic hydrolysis.Personality Cat Eye Sunglasses(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDIN127 Stainless Steel Spring Lock Washer, but not shown inSnf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing Admixture,ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline, and also free amino acids (Whitaker et al., 1963).UV Coated PVC Panel for Interior Wall Board. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3D Embossed Iron on Label Clothing Heat Transfer Label,1996).
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Wet Wire Drawing Machine for High Carbon Mattress Spring WireandDIN7504 Stainless Steel Pan Head Philips Self Drilling Screwshow the gel chromatographic separation of soluble protein with Sephadex G-Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring..Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device, and another peak at 45 mL not shown inHuaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord, for peptides lower than 3000 Da.Wholesale 30W LED Down Lights with Budget Price(Condell et al.,1993) .
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Embossed Textured Wallpaper for Solvent Printing, Ve, and Kavcalculated as discussed previously.Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray,4-14 Manual Staple Gun with Staples for Hand Tools. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile) (Reis et al.,1989;Sc-2400 Automatic Multi Function Sosn Side Drilling Machine.,1991;Glass Food Jar & Plastic Lid.,1991).500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3)(1992), were lower, except for cystine and tyrosine,Smallest Portable Waste Transformer Oil Purifier Machine Zy-6.
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CONCLUSIONS
Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.
ACKNOWLEDGMENT
The authors would like to thank FNMA/MMA , CAPES, and CNPq (Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel) for the financial support.
REFERENCES
High Speed Automatic Packing Machine (GK-650 BA), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Air Brake Hose Line For Truck Bus]
Brown, E. M; Thompson, C .J.,5000-5900MHz 4G Built-in Automotive Antenna, M.M.(1994),Glass Lined Reactor for Chemical Factory.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai]
Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale]
Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM Metal Stamping Stainless Steel Parts with CNC Machining. Analytical Biochemistry212,436-445[4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone]
Dalev, P.G. and Simeonova, L.S.(1992),Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper. Biotechnology letters 14,6, 531-534[Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China]
Heidemann, E.(1997),Supermarket Plastic T-Wire Basket Swing up Price Label Holder.Leder 48,(5),99-104[Products]
Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Imirootree 250ml Glass Jar with Lids for Cosmetic Cream]
NPK 15 15 15 Compound Fertilizer at a Hot Selling, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine.Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7. USP/SP[Factory Supply 100% Natural Epimedium Extract for Male Health]
O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool]
Reis, M. and Beleza, V.(1989), Utilization of leather waste-Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Acrylic Beer Display Racks with LED]
Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[100% Silver Fiber Clothing]
Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[T6 Outdoor Waterproof Tactical Flashlight]
Degradable PLA Plastic Bottle, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale China Merchandise 12V Horizontal Mini Camping Fridge-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[24L Thermoelectric Cooler and Warmer Car Mini Car Fridge]
Degradable PLA Plastic Bottle,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws]
Degradable PLA Plastic Bottle, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House, Part I.No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157]
2D LED Rope Lighting Motif Lights for Christmas Decoration, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls.10ml Dropper Bottle for Skin Care Packing, 175-184[Anti-Reflective Anti-Finger Touch Monitor Wholesaler Quotes & PriceList]
Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Different Size Glass Test Tubes]
Whitaker,J.R.(1963),Custom High Precision Micro Machining Service Brass Micromachining Parts. Analytical Chemistry35(12), 1950-1953[Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark]
Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.
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