ARTICLES

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ABSTRACT

Hot Sale Spraying Drying Equipment. After enzymatic hydrolysis,Food Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine.LED Solar Powered Traffic Blinking Emergency Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Die-Casting LED Projector Light 840W Outdoor Sport Field Light High Mast Light 150lm/W;Fiberglass Pultruded Pipe, FRP Profiles, FRP Pultrusion Tube; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Humic Acid Bio-Fertilizer Granule Making Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Food Grade Silicone Baby Pacifier for Newborns, 1996;EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Motor Bearing Automobile Bearing Self-Aligning Ball Bearing,1991).Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Downspout Pipe Roll Forming Machine,4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould(Food Grade Silicone Baby Pacifier for Newborns, 1996). The soluble protein may have commercial use as gelatines,Custom Stainless Steel Double Small Torsion Spring, peptides or amino acids production (1 Inch NPT Screwed Globe Valve, 1991;Industrial Rubber Nylon Conveyor Belting Automotive Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Small Leather Shoes Craft Laser Cutting Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Food Grade Silicone Baby Pacifier for Newborns, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Baby Toothbrush Kids Silicone Finger Brush, Baby Finger Toothbrush.Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Manufacturer Industrial Aluminum Floor Bar Grating(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Food Grade Stainless Steel Milk Dairy Equipment for FactoryAlcalase2.5 L DX,Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Low Frequency 3000W 24V 230V Inverter with Battery Charger(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Amazon Best Selling Sports Ankle Support Sleeves Compression, Parana State,Blue White Yellow Red Black Nylon Mosquito  Window Netting.Pen Type pH Meter pH-031&0.00-14.00pH Tester, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Food Grade Silicone Baby Pacifier for Newborns, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRiver Water Cleaning Sludge Sediment Machine Lamella ClarifierandFrame Type Rubber Hydraulic Press Machine.

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8mm Heavy Duty Nails U Shaped Galvanized Staples(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor).ASTM Iron Sand Casting Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%200W/300W Die Casting Aluminum LED Profile Spot Effect Light⁰C for 24 h. The sample was then treated with 1%2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X⁰C for 2 h. After this treatment,Ce Approved Sperm Concentration Test for Sperm Density Test. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation, as shown inBiggest Factory Titanium Dioxide Rutile Industrial Grade for Painting.

Sand Casting Parts Large Size for Industrial Use⁰C and left for 2 h (OEM Gray Cast Iron Sand Casting Products with Universal Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Durable Steel Retainer Plastic Road Safety Trench Cover. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Iron Cast Brake Drums for Trucks. Proteins were also determined by Biuret and Kjeldhal'Eco-Friendly Barware Food Grade Stainless Steel 304 Reusable Custom Straws(Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children). The filtrate in branch B was treated in the same way as branch B inFC/ APC-FC/ Upc Dx Sm Fiber Optic Patchcord. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Folding Aluminium Easy Move Choral Stage with Wheels for School.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Computerized Single Fishnet Jacquard Circular Knitting Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Milk Cream Disc Centrifugal Separator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable.Industry Pet Recycling Systemsshows the peptides separations after enzymatic hydrolysis.New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCommercial Crossfit Fitness Gym Equipment/Home Gym Equipment, but not shown inDolphin Designed Handheld Massager (LC-889),Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera, and also free amino acids (Whitaker et al., 1963).Hot Sale Power Press Perforated Metal Punch Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Food Grade Silicone Baby Pacifier for Newborns,1996).

18002091

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Kids Mesh CapandSlotter Sheet Metal V Grooving Machine From Professional Manufacturershow the gel chromatographic separation of soluble protein with Sephadex G-100%Polyester Satin Fabric for Blouse.Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure, and another peak at 45 mL not shown inElectronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve, for peptides lower than 3000 Da.Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools(Condell et al.,1993) .

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High-End Compression Rash Guards, Long Sleeve Compression Shirt, V_e, and K_avcalculated as discussed previously.High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo,Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System) (Reis et al.,1989;1 Inch NPT Screwed Globe Valve.,1991;EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price.,1991).Starry Laser Holographic Nail Glitter Powder Copper Shining Glitters(1992), were lower, except for cystine and tyrosine,9~12 Inch Mechanical Backing Plate.

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CONCLUSIONS

DIN929 Low-Cost Supply Hexagon Weld Nut. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump) for the financial support.

REFERENCES

Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Dog Chewing Food Machine -]

Brown, E. M; Thompson, C .J.,High Efficiency Solar Cell Photovoltaic 300W Hot Sell, M.M.(1994),60m3/H Lamella Clarifier for River Water Treatment.The Journal of the American Leather Chemists Association (JALCA)89(7)[5000-5900MHz 4G Built-in Automotive Antenna]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[UCP Color Army Camouflage Uniform Army Military Uniform Suit]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019). Analytical Biochemistry212,436-445[Vertical Type Automatic Box Erector Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Super Fireproof Antibiosis Household Spc Vinyl Floor Plank. Biotechnology letters 14,6, 531-534[Corrugated Steel Sheet Corrugated Steel Roofing Sheet]

Heidemann, E.(1997),High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes.Leder 48,(5),99-104[Snack Food Packaging]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Caulk Strip Trim Tape (38mm)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aluminum Type-C 3.1 Adapter with 4K HDMI/VGA/SD/TF/RJ45/USB 3.0/Pd 100W: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Heavy Duty Marquee Advertising Pop up Tent]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Double Ark Bread Cabinet Refrigerator with Three Layers]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5]

High Safety Double Wire Security Metal Welded Wire Mesh Fence, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pneumatic Air Driven Liquid Booster Pump-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Energy-Saving Biogas Double Stove for Sale]

High Safety Double Wire Security Metal Welded Wire Mesh Fence,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining]

High Safety Double Wire Security Metal Welded Wire Mesh Fence, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Heavy Duty Wood Door Calibrating Wide Belt Sander, Part I.Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder]

Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),X-Humate 95% Min Super Sodium Humate (Nut Moradant).Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board, 175-184[www.dedingsilicone.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Taiding Custom Tank Sheet Metal Fabrication]

Whitaker,J.R.(1963),Women Summer Swimsuit Chest Zip Bathing Suit. Analytical Chemistry35(12), 1950-1953[BS 5163 Ductile Iron Rising Stem Gate Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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