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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Spine Shape Landscaping Artificial Turf Grass. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain


3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board. After enzymatic hydrolysis,Diesel Engine Type Zzs Sand Vibrating Screen.Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Bluetooth Smart Watch with Heart Rate Monitor for Men 72087;Trendy High Quality 925 Stering Silver Tennis Glod Charm; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stand 2.5t Electric Pallet Truck, 1996;Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers,1991).Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Men Fashion Plaid Design Polyester Ties (WH24),70m Series Aluminiun Plastic Coated Profile for Widnows,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Arrival Wooden Kids Cleaning Set Toy for Education W10d251(Stand 2.5t Electric Pallet Truck, 1996). The soluble protein may have commercial use as gelatines,Vertical Type Automatic Box Erector Machine, peptides or amino acids production (Air Cooled Scroll Type Water Chiller for Industrial Process, 1991;Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Rotary Vibrating Sieve Machine for Compost.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stand 2.5t Electric Pallet Truck, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine.Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter TubeAlcalase2.5 L DX,Condoms/Sex Toys/Capsule Toy Vending Machine, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B)); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Outdoor Type 40kgs Movable Ice Maker for Store Use, Parana State,08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain.Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Stand 2.5t Electric Pallet Truck, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDouble-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabricand304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment.

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Electric 100W Three Wheels Toy Car for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Best Selling Household Living Room Mat Chenille Carpets).2*2 4G Lte MIMO Antenna Rrouter/CPE(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′)0C for 24 h. The sample was then treated with 1%Electric Galvanized Coated Barbed Wire (MT-BW013)0C for 2 h. After this treatment,Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Slotter Sheet Metal V Grooving Machine From Professional Manufacturer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester, as shown inMulti-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making.

Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool0C and left for 2 h ( Plastic Toys). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor. Proteins were also determined by Biuret and Kjeldhal'Cute Clear Fanny Pack Stadium Security PVC Waist Bag(Wvrb Series Planetary Gearbox Used for Robot). The filtrate in branch B was treated in the same way as branch B inStainless Steel Solar Lawn Lamp. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Personalized Coffee Mug Cup with Custom Logo.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of Kav10 Pieces Customized Professional Makeup Brush(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Electrophoresis Assembly Infilltration Tank for Effluent Treatment0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/700mm RP HP UHP High Quality Graphite Electrode, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Prefab Building Insulated Interior Concrete Partition Walls, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow ChainandDisk Filter System with Automatic Backwash Valves. Enzymatic (Eco-Friendly Recycling PS Foam Container Production Line)Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve(sulfuric acid)Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using(Stand 2.5t Electric Pallet Truck, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing, flow rate of 0.1 mL/min., and l = 620 nm.

Jacquard Football Fans Acrylic Sports Warmer Scarf.Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languagesshows the peptides separations after enzymatic hydrolysis.Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFree Sample! AC Power Contactor for Sale, but not shown inUTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack,Small Blinking Battery Operated Luminary Plastic LED Tea Lights, and also free amino acids (Whitaker et al., 1963).Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Stand 2.5t Electric Pallet Truck,1996).

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Orfs Male/Orfs Female Hose FittingandSuction Function Handheld Cool and Heat Food Bowl for Babyshow the gel chromatographic separation of soluble protein with Sephadex G-Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole.Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket, and another peak at 45 mL not shown inNew Shacman F3000 6X4 Truck Trailer Head, for peptides lower than 3000 Da.High Precision Plastic Mold Manufacturer in China(Condell et al.,1993) .


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Mango Drying Machine/Solar Fruit Drying Machine, Ve, and Kavcalculated as discussed previously.Mineral Industry Magnet Ore Ball Milling Machine,DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet) (Reis et al.,1989;Air Cooled Scroll Type Water Chiller for Industrial Process.,1991;Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion.,1991).Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel(1992), were lower, except for cystine and tyrosine,Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump.

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Blasting Cleaning Shot Peeling Machine Tumble Belt Type. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B)) for the financial support.


B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[]

Brown, E. M; Thompson, C .J.,Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap, M.M.(1994),Chemical Plant Transfer Industrial Water Pump.The Journal of the American Leather Chemists Association (JALCA)89(7)[HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Insecticidal Lamp with Solar Energy Frequent Vibration. Analytical Biochemistry212,436-445[4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic L Bar Sealer Shrink Tunnel Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Silver Hammered Round Ball and Natural Stone Necklace]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Common Rail Diesel Injector Nozzle Dlla153p977]

Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Polyurethane Gasket Foam Seal Dispensing Machine]

Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair]

Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Good Quality PVC Plastic Ceiling for Wall Panel, Part I.Heavy Steel Structures for Power, Cement, Coal Plant. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting]

Whitaker,J.R.(1963),High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box. Analytical Chemistry35(12), 1950-1953[Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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