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ABSTRACT

Good Quality Xanthan Gum Application of Food with Best Price. After enzymatic hydrolysis,Wet Wire Drawing Machine for High Carbon Mattress Spring Wire.120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Effluent Treatment Ferrous Sulphate Heptahydrate 98%;Rechargeable 9V Li Ion Battery with Micro USB Charging Port; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Transport System China Manufacturer for Table Belt Conveyor, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal, 1996;Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Mini LED Flood Light SMD Flood Light 50W,1991).Ferrite Core for Power Transformer (Ee16e). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel,China Manufacturer Wallpaper House Decoration Wall Paper,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Flexible Magnetic Rubber Sheet Soft Magnetic Tape(Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal, 1996). The soluble protein may have commercial use as gelatines,Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass, peptides or amino acids production (Electric Wine Bottle Opener K020, 1991;Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Spine Shape Landscaping Artificial Turf Grass. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain.3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Diesel Engine Type Zzs Sand Vibrating Screen(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts).Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Men Fashion Plaid Design Polyester Ties (WH24)⁰C for 24 h. The sample was then treated with 1%70m Series Aluminiun Plastic Coated Profile for Widnows⁰C for 2 h. After this treatment,New Arrival Wooden Kids Cleaning Set Toy for Education W10d251. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Vertical Type Automatic Box Erector Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Air Cooled Scroll Type Water Chiller for Industrial Process, as shown inKorean Integrated CTO Activated Carbon Filter for Water Treatment.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avModern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Condoms/Sex Toys/Capsule Toy Vending Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe PriceandOutdoor Type 40kgs Movable Ice Maker for Store Use. Enzymatic (Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts)Electric 100W Three Wheels Toy Car for Sale(sulfuric acid)2*2 4G Lte MIMO Antenna Rrouter/CPE(Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′), flow rate of 0.1 mL/min., and l = 620 nm.

Electric Galvanized Coated Barbed Wire (MT-BW013).Promotional Customized PP Hand Fanshows the peptides separations after enzymatic hydrolysis.Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAuto Plastic Part Hardware Plastic Injection Mold, but not shown inHerbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine,Slotter Sheet Metal V Grooving Machine From Professional Manufacturer, and also free amino acids (Whitaker et al., 1963).Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal,1996).

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CONCLUSIONS

Electrophoresis Assembly Infilltration Tank for Effluent Treatment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Trendy High Quality 925 Stering Silver Tennis Glod Charm) for the financial support.

REFERENCES

700mm RP HP UHP High Quality Graphite Electrode, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[OEM/ODM Supplier Appliance Voltage Protector - 12V 4000W Modified Sine Wave Inverter - Ligao]

Brown, E. M; Thompson, C .J.,Prefab Building Insulated Interior Concrete Partition Walls, M.M.(1994),Disk Filter System with Automatic Backwash Valves.The Journal of the American Leather Chemists Association (JALCA)89(7)[Soldering Machine LED Module PCB Hot Air Soldering Station]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ground Finish Molybdenum Tzm Rod]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Eco-Friendly Recycling PS Foam Container Production Line. Analytical Biochemistry212,436-445[ 20%Sc]

Dalev, P.G. and Simeonova, L.S.(1992),Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve. Biotechnology letters 14,6, 531-534[Manufacturer of Soda Ash Dense 99 2% Price]

Heidemann, E.(1997),Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using.Leder 48,(5),99-104[Poe Cable Kit Power Over Ethernet Cable]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Barbed Wire Right off The Border Wall to Protect Homes]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Magnifying Mirror Shaving Mirror 2X]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Flour Packing Machine 25kg]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Outdoor Concert Stage Equipment Aluminum Stage Portable Stage]

Small Blinking Battery Operated Luminary Plastic LED Tea Lights, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine]

Small Blinking Battery Operated Luminary Plastic LED Tea Lights,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery]

Small Blinking Battery Operated Luminary Plastic LED Tea Lights, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole, Part I.Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine]

High Precision Plastic Mold Manufacturer in China, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mango Drying Machine/Solar Fruit Drying Machine.Mineral Industry Magnet Ore Ball Milling Machine, 175-184[Best Hot Selling Home Using Gold Thread]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[FTTX Solution Provider Fiber Optical 12cores MPO Cassette]

Whitaker,J.R.(1963),DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut. Analytical Chemistry35(12), 1950-1953[Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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