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ABSTRACT

300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction. After enzymatic hydrolysis,BS 5163 Ductile Iron Rising Stem Gate Valve.CNC Aluminum Sheet Press Brake. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Bio Media, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine, 1996;Platform Trolley with 150kg Capacity, 4 Castor Wheel., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine,1991).LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,All Size Colored Tapping Hex Head Self Drill Screw Wood Screw,Pre-Engineered Cage Broiler Poultry Farm Building,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Alumina Ball with High Quality(Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine, 1996). The soluble protein may have commercial use as gelatines,PVC Textile Fabric for Outdoor Sports Waterproof Backpack, peptides or amino acids production (White 4*8FT 18mm PVC Foam Board for Cabinets Use, 1991;Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Powerful Anti-Stress, Strong Flexibility Spoke Wire.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Modern American Style Beech Solid Wood Kitchen Cabinet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hero Brand Polypropylene Bags Machine.CNC Machining Metal/Aluminum/Brass Auto Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Surprise! Range Hood Free! ! ! Juice Cart(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant, Parana State,10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper.Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theModern Carbon Fiber Bathroom Vanity Combo with Mirror CabinetandGood Quality NBR70 O-Ring with Reasonable Prices.

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En1092-1 05 Pn16 Blind Flat Face Flange(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024).Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Peristaltic Pump with Connecting The High Speed Tubular Separator⁰C for 24 h. The sample was then treated with 1%High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding⁰C for 2 h. After this treatment,LED Bulb Light with Remote Control RGB Christmas Bulb. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Home Decoration Kids Toy Promotion Eyeglass Plastic Mould's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display, as shown in4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring.

Good Price Gi/PPGI Roofing Sheet in China⁰C and left for 2 h (4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Multipurpose Wireless PA Amplifier Lectern PA Speaker. Proteins were also determined by Biuret and Kjeldhal'3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads(I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case). The filtrate in branch B was treated in the same way as branch B inChina Supplier Motorcycle Accessories Aluminum CNC Chain Adjuster. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av12ml Round Shape Empty Clear Nail Polish Glass Bottle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Skytone 4 Channels 3500W Each DJ Power Amplifier, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage TruckandPVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels. Enzymatic (2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture)Imirootree 250ml Glass Jar with Lids for Cosmetic Cream(sulfuric acid)Double Wine Carrier Bag Gift Box Tote(Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts, flow rate of 0.1 mL/min., and l = 620 nm.

Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord.Skytone 4 Channels 3500W Each DJ Power Amplifiershows the peptides separations after enzymatic hydrolysis.Flexible PVC Granules for Cable, Wire and Insulation(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTwo Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine, but not shown inAutomatic L Bar Sealer Shrink Tunnel Machine,7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security, and also free amino acids (Whitaker et al., 1963).Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine,1996).

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Polyurethane Gasket Foam Seal Dispensing MachineandOutdoor Dining Table Set Stainless Steel Table Mesh Fabric Chairshow the gel chromatographic separation of soluble protein with Sephadex G-Brass Pump Spare Parts for Circulating Pump.Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes, and another peak at 45 mL not shown inHeating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation, for peptides lower than 3000 Da.Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi(Condell et al.,1993) .

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CONCLUSIONS

Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG) for the financial support.

REFERENCES

Cylindrical Shape Beer Glass Bottle for Wine Storage, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Coffee In Paper Cup]

Brown, E. M; Thompson, C .J.,Plastic Injection for OEM Product, M.M.(1994),HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024.The Journal of the American Leather Chemists Association (JALCA)89(7)[Heating Refrigerated Low Temperature Cooling Circulator Water Chiller]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Design Plush Panda Animal Slippers Comfort Foam Support by]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event. Analytical Biochemistry212,436-445[Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg]

Dalev, P.G. and Simeonova, L.S.(1992),Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen. Biotechnology letters 14,6, 531-534[5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building]

Heidemann, E.(1997),Activated Carbon Strong Effect Peeling off Face Mask Mud.Leder 48,(5),99-104[www.rotec-hid.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Halal Empty Gelatin Capsule for Medicine]

Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Type Price Prefabricated Roof Tile in Sudan.Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion. USP/SP[Aluminum Mushroom Rround Head Solid Rivet (Round head)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[22*30mm 12SMD 5050 LED Reading Light for Car]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Supply 100% Natural Epimedium Extract for Male Health: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[DIN127 Stainless Steel Spring Lock Washer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Access Control System Manufacturer Cheap Price Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Snf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing Admixture]

Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Private Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM]

Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wet Wire Drawing Machine for High Carbon Mattress Spring Wire]

Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Top10 1080P H. 264 P2p CCTV WiFi IP Camera, Part I.Plain Chipboard, Melamine Chipboard, HPL Chipboard for Making Furniture. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw]

Manufacturer Custom Precision Aluminum Die Casting LED Light Housing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate.1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S), 175-184[m.songligroup.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord]

Whitaker,J.R.(1963),Full Color Child Book Printing Service. Analytical Chemistry35(12), 1950-1953[Valve Bag Dry Mortar Bagging Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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