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vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes. After enzymatic hydrolysis,Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments.Factory Supply Cheap Custom Bleached White Cotton Gloves. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle;304 &314 Stainless Steel Seamless Bending Tube; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Grey Full-Zip Cotton Mens Hoodies, 1996;Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (7inch Industrial Tablet Computer Naked Resistance Touch Screen,1991).Glass Door Passenger Sightseeing Elevator for Sale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys,12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple(Custom Grey Full-Zip Cotton Mens Hoodies, 1996). The soluble protein may have commercial use as gelatines,Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase, peptides or amino acids production (Fcy30 Transport Mini Tipping Dumper, 1991;Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Premium Grade Rubber Cloth Duct Tape.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sud1000h Poly Welder Butt Fusion Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Grey Full-Zip Cotton Mens Hoodies, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Customized Anti-Rust Torsion Spring.Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated, Parana State,Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price.Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Custom Grey Full-Zip Cotton Mens Hoodies, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theImpact Sound Isolation Wall ClipandAISI 410 Stainless Steel Galvanized Wire for Making Scourer.

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Industrial Carbon Steel Wire Hose Hydraulic Pipe(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter).24k Gold Whitening Anti-Spot Mask(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Clay Tiles Making Machine with Latest Technology0C for 24 h. The sample was then treated with 1%DIN Standard Gratings/Gavlanized Steel Grating0C for 2 h. After this treatment,50mm Magnetic River Water Sewage Flow Meter for Acid. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PE Biodegradable Sheet Product Line/Single Screw Extruder's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)27L/Min Water Exchanger Heat Pump Mold Temperature Machine, as shown inB45 2-Stroke Petrol Grass Cutting Brush Cutter Machine.

Felt Belt for Textile Compactor0C and left for 2 h (Fully Automatic C Z Purlin Cold Roll Forming Machinery). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Metal Soft Enamel Souvenir Metal Medal Sport Medallions. Proteins were also determined by Biuret and Kjeldhal'20L Water Tank Lid Mold 3D Printing Samples Available(Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection). The filtrate in branch B was treated in the same way as branch B inEye Bolt With Oval Link. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale High Quality Metal Car Mount Holder Phone Holder.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavHigh Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Design Multi-Color Silicone Coin Purse for Lady, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


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Water Treatment Anthracite.Chicken Slaughtering Machine for Saleshows the peptides separations after enzymatic hydrolysis.Heat Treated Welding Protection Glassfiber Cloth Ht800(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm, but not shown inUSB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug,Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring, and also free amino acids (Whitaker et al., 1963).Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Custom Grey Full-Zip Cotton Mens Hoodies,1996).

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Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer, Ve, and Kavcalculated as discussed previously.Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator,Injector Pump Calibration Test Bench. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Top Quality Galvanized Iron Wire as Binding Wire) (Reis et al.,1989;Fcy30 Transport Mini Tipping Dumper.,1991;Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook.,1991).ASME/ASTM Butt-Welding Stainless Steel Plate Flange(1992), were lower, except for cystine and tyrosine,500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank.

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Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (PP Woven Bag, Rice, Beans, Plastic Bag) for the financial support.


Polyester / Polypropylene Woven Geotextile for Road Construction, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Advertising Player 43 Inch Touch Advertising Player Portable Player Full HD LED Ad Player Video Best Quality LED Digital Signage]

Brown, E. M; Thompson, C .J.,Granite Stone Jaw Crusher of Mining Machine, M.M.(1994),Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor.The Journal of the American Leather Chemists Association (JALCA)89(7)[Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer. Analytical Biochemistry212,436-445[Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Professional Stainless Steel Ethanol Processing Plant From Cassava]

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Reis, M. and Beleza, V.(1989), Utilization of leather waste-Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cake Baking Pans]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[NPK 15 15 15 Compound Fertilizer at a Hot Selling]

Whitaker,J.R.(1963),3 Layer Round Tea Tin with Air Tight Lid. Analytical Chemistry35(12), 1950-1953[Prefab Steel Structure Shed for Storage Warehouse]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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