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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould

ABSTRACT

0.1mm Tungsten Carbide 2f Single Flute Micro End Mill. After enzymatic hydrolysis,Agriculture Large Span Commerical Steel Frame Greenhouse.Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags;Ss400 Corner Metal Sheet Bar Galvanized Angle Iron; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer, 1996;Chinese Jbcz Handy Operation Threading Machine and Coupler., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Promotional Reusable Foldable PP Non Woven Shopping Bag,1991).Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Price Printing Doy Pack Bag,Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes(Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer, 1996). The soluble protein may have commercial use as gelatines,Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price, peptides or amino acids production (High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17, 1991;Engine Parts Rubber High Quality Timing Belt 111ru25, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Interior Metal 3D Bee Garden Ornament Decoration., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Disposable Non-Woven Dry Gloves Wipes Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting.Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Bottom Pushing for Fire Extinguisher Hot Spinning Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

90m3 Per Hour Mobile Concrete Pump, Parana State,Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould.China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePlain ChipboardandCNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories.

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FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4).All Weather Rattan Handwoven Swing Outdoor Home(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pond Self Priming Booster suction  Water Pump with Pressure Switch⁰C for 24 h. The sample was then treated with 1%Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter⁰C for 2 h. After this treatment,Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree, as shown in14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags.

School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056)⁰C and left for 2 h (Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation. Proteins were also determined by Biuret and Kjeldhal'0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine(Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7). The filtrate in branch B was treated in the same way as branch B inwww.sunhogloves.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,125kHz RFID Hotel Key Card with T5577 Chip.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale High Pressure 80L Nitrogen Gas Cylinder⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/10mm Red Green Yellow Warning China Supplier Signal Lamp, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam.Printed Wristband Cotton Wristband RFID Woven Wristbandshows the peptides separations after enzymatic hydrolysis.Ductile Iron Standard Brackets Pipe Hanger(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRound Shape All Color Pets Feeding Dog Bowl of China Supplies, but not shown inReverse Rolled Wedge Wire Screen/ Jonson Screen Plate,Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers, and also free amino acids (Whitaker et al., 1963).ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer,1996).

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CONCLUSIONS

Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Produce N2o for Medical Gas Equipments) for the financial support.

REFERENCES

Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Plastic Pink Gold Poly Mailer Packing Mailing Bag for Courier]

Brown, E. M; Thompson, C .J.,High Strength Rigid PVC Plastic Foam Board for FRP, M.M.(1994),Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Home Use Standing Modern Floor Lamp (KAF6108)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall. Analytical Biochemistry212,436-445[Hot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts]

Dalev, P.G. and Simeonova, L.S.(1992),Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve. Biotechnology letters 14,6, 531-534[ 230V]

Heidemann, E.(1997),Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould.Leder 48,(5),99-104[High Quality Bluetooth Speaker Plastic Shell Injection Mould Maker]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain]

Vertical Type Caseformer Machine WJ-LKX-15, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps.New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels. USP/SP[China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[10ton Telescopic Hydraulic Truck Crane]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Rainsky Ceiling Mounted Stainless Steel Shower Heads]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.ritecmachine.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts]

Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),ADSS Newest Ultra Therapy Skin Tightening 3D Hifu-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X]

Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[DIN929 Low-Cost Supply Hexagon Weld Nut]

Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap, Part I.Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Home Decoration Candle Glass Holder for Table Decorative]

High Quality Ceramic Chrome Hybrid Bearing Oil, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown.CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder, 175-184[Chinafireworks]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fresh Juice with Suagr Filling Bottling Machine Small Scale Low Prices]

Whitaker,J.R.(1963),Digger Arm Small Wheel Loader (HQ908) with CE. Analytical Chemistry35(12), 1950-1953[CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly ValveAuthor for correspondence