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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer. After enzymatic hydrolysis,Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration.Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat;River Water Cleaning Sludge Sediment Machine Lamella Clarifier; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Polyester Coated Stainless Steel Ladder Type Metal Ties, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device, 1996;SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Copper Laminated Steel Strip T2/C11000,1991).Halal Empty Gelatin Capsule for Medicine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm,Gym Fitness Exercise Sporting Body Building Bicep Press,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck(2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device, 1996). The soluble protein may have commercial use as gelatines,Metal Steel Frame Membrane Structure Car Parking Shed Cover Design, peptides or amino acids production (Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size, 1991;300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Polyester Dust Sport Gym Fabric Shoes Drawstring Bag.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 101.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price.Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans, Parana State,5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam.Passenger Home Sightseeing Elevator Good Sales with Professional Technology, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLED Solar Powered Traffic Blinking Emergency LightandBlack Steel ASTM A192 Seamless Pipe Sch120.

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Vacuum Stainless Steel Casting Products(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Korean Style PE Foam 3D Wallpapers).Chicken Farming Materials Poultry Battery Cages Layer Battery Cage(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer0C for 24 h. The sample was then treated with 1%I-Tech 12000HD 2u Space Digital Professional Power Amplifier0C for 2 h. After this treatment,Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Silicone Reusable Food Storage Bag China Manufacturer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Polyurethane Gasket Foam Seal Dispensing Machine, as shown in50m Depth Long Range Metal Detector with Reliable Quality.

Imee Print Customized Printing Agenda Printing Nb210C and left for 2 h (Customized Anti-Rust Torsion Spring). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,High Performance Multifunctional Crawler Mini Skid Steer Loader. Proteins were also determined by Biuret and Kjeldhal'Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar(High Quality Ceramic Chrome Hybrid Bearing Oil). The filtrate in branch B was treated in the same way as branch B in250 Rpm Synchronous Motor. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Chunke Portable UV Water Sterilizer for Water Purifier 150t.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavStainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stop Dog From Jumping or Climbing Your Chain Link Fence.0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/SGS Direct Thermal Label for Supermarket, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Water Filter Polyester Non-Woven Fabric, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


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Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set.Good Price Welding Electrode (e7018/e7018-1)shows the peptides separations after enzymatic hydrolysis.Energy-Saving Biogas Double Stove for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Printed Socks, but not shown inStainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price,Valve Bag Dry Mortar Bagging Machine, and also free amino acids (Whitaker et al., 1963).High Quality 500m 100lb Super Strong Braided Fishing Line. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device,1996).

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Pet PP Rope and Broom Wire Drawing Machineand100% Acrylic Knitted Sports Woven Scarfshow the gel chromatographic separation of soluble protein with Sephadex G-20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China.37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light, and another peak at 45 mL not shown inYellow Hazardous Spill Response Chemical Spill Kits, for peptides lower than 3000 Da.Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags(Condell et al.,1993) .

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PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint, Ve, and Kavcalculated as discussed previously.Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine,Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Easy Operation Low Investment Cassava Silo) (Reis et al.,1989;Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size.,1991;SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder.,1991).Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch(1992), were lower, except for cystine and tyrosine,Custom Stainless Steel Jewelry Production by Metal Injection Molding Process.

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Factory Directly Selling Engine Standard Spark Plug. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plastic Food Box Container Covers by Injection Mould Mold Tool) for the financial support.


Custom Logo Silicone Wristband, Rubber Wrist Bands, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ladies Big Size Underwear Panties]

Brown, E. M; Thompson, C .J.,Custom All Kinds of Acrylic Shoe Display Racks, M.M.(1994),Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide.The Journal of the American Leather Chemists Association (JALCA)89(7)[Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Full Color Child Book Printing Service]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale. Analytical Biochemistry212,436-445[1000base Single Mode 1310nm LC SFP Module 20km 1.25g]

Dalev, P.G. and Simeonova, L.S.(1992),Medical Supply U40 Disposable Insulin Syringe. Biotechnology letters 14,6, 531-534[China PA System Power Amplifier with Enough Rated Power]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sud1000h Poly Welder Butt Fusion Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Precision Tungsten Carbide Machine Customized Cutting Tool: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Activated Carbon Strong Effect Peeling off Face Mask Mud]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Travelling Portable O2 Concentrator Jay-1]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine]

I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Maleic Anhydride CAS 108-31-6-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Nutrition Enhancer Environmental Nutritional Yeast Powder]

I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple]

I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2000mj Q Switched for Pigment Removal with Ce Certification, Part I.Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W]

Whitaker,J.R.(1963),20kv 50kVA High Voltage Dry Type Power Supply Transformer. Analytical Chemistry35(12), 1950-1953[Wholesale Wireless Cheapest Android 2D Barcode Scanner]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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