ARTICLES

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ABSTRACT

Modern American Style Beech Solid Wood Kitchen Cabinet. After enzymatic hydrolysis,Hero Brand Polypropylene Bags Machine.CNC Machining Metal/Aluminum/Brass Auto Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.316L Cold Rolled Stainless Steel Coil 316L Inox, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG, 1996;Fully Automatic C Z Purlin Cold Roll Forming Machinery., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant,1991).Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,En1092-1 05 Pn16 Blind Flat Face Flange,Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Peristaltic Pump with Connecting The High Speed Tubular Separator(Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG, 1996). The soluble protein may have commercial use as gelatines,High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding, peptides or amino acids production (LED Bulb Light with Remote Control RGB Christmas Bulb, 1991;Home Decoration Kids Toy Promotion Eyeglass Plastic Mould, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Good Price Gi/PPGI Roofing Sheet in China.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Multipurpose Wireless PA Amplifier Lectern PA Speaker.3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Skytone 4 Channels 3500W Each DJ Power Amplifier, Parana State,Powerful Anti-Stress, Strong Flexibility Spoke Wire.Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Drivingand3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board.

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PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Original Stainless Steel Singer Brand Sewing Machine Needles).2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Imirootree 250ml Glass Jar with Lids for Cosmetic Cream⁰C for 24 h. The sample was then treated with 1%Double Wine Carrier Bag Gift Box Tote⁰C for 2 h. After this treatment,(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Flexible PVC Granules for Cable, Wire and Insulation, as shown in19mic Black Cast PE Stretch Film for Pallet Wrapping.

7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security⁰C and left for 2 h (Imee Print Customized Printing Agenda Printing Nb21). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Brass Pump Spare Parts for Circulating Pump. Proteins were also determined by Biuret and Kjeldhal'Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes(SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving). The filtrate in branch B was treated in the same way as branch B inSuperbright LED C40 E26 Base Candle Shape Light Bulbs. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLantern for Garden Decoration(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Shacman F3000 6X4 Truck Trailer Head⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Plain Chipboard, Melamine Chipboard, HPL Chipboard for Making Furniture, V_e, and K_avcalculated as discussed previously.Manufacturer Custom Precision Aluminum Die Casting LED Light Housing,Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer) (Reis et al.,1989;LED Bulb Light with Remote Control RGB Christmas Bulb.,1991;Fully Automatic C Z Purlin Cold Roll Forming Machinery.,1991).1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S)(1992), were lower, except for cystine and tyrosine,Full Color Child Book Printing Service.

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CONCLUSIONS

1.5 Inch High Pressure Petrol/Gasoline Water Pump. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (M4X10 Round Head Core Pulling Aluminum Nail) for the financial support.

REFERENCES

Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[API 5CT J55 N80 P110 Casing and Drill Seamless Pipe with Thread Couplings for Extraction]

Brown, E. M; Thompson, C .J.,Equal and Unequal High Quality Hot Rolled Steel Angle, M.M.(1994),Halal Chicken Slaughter Machine for Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Reflective Hand Hold Thermoplastic Road Marking]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop. Analytical Biochemistry212,436-445[Economical Residential Home Automatic Aluminum Rolling Shutter Door]

Dalev, P.G. and Simeonova, L.S.(1992),2019 Opt Shr IPL E-Light Beauty Hair Removal Machine IPL Shr. Biotechnology letters 14,6, 531-534[2000mj Q Switched for Pigment Removal with Ce Certification]

Heidemann, E.(1997),Sarm Steroids Ostarine / Mk-2866 / Mk2866 Powder for Bodybuilding CAS 841205-47-8.Leder 48,(5),99-104[2019 Personal Design Plastic of Laundry Basket Mould]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Xanthan Gum for Personal Care and Cosmetic Applications: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Waterproof EVA Rubber Roofing Material for Shingle Building]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sheet Metal Laser Cutting Machine for Pipe (2000W)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Brass Pump Spare Parts for Circulating Pump]

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Manual Vertical Car Key Cutting Machine for Locksmith,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir]

Manual Vertical Car Key Cutting Machine for Locksmith, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069), Part I.Transparent Color Stretch Wrap Film Manual Operate Wrap Film. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish]

Good Price Welding Electrode (e7018/e7018-1), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ep/Nn/EPP Industrial Rubber Conveyor Belt.Tpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing, 175-184[Film 595 Pvc Ceiling]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel Counter Top Working Table Chiller]

Whitaker,J.R.(1963),New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238. Analytical Chemistry35(12), 1950-1953[Diesel Engine Type Zzs Sand Vibrating Screen]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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