ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Chemical Plant Transfer Industrial Water Pump. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Insecticidal Lamp with Solar Energy Frequent Vibration

ABSTRACT

Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286. After enzymatic hydrolysis,Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics.Galvanized Square Washer with Timber Constructions, DIN436DIN436. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist, 1996;Good Quality PVC Plastic Ceiling for Wall Panel., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Heavy Steel Structures for Power, Cement, Coal Plant,1991).Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CE Approved Manual Heat Press Machine,T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box(Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist, 1996). The soluble protein may have commercial use as gelatines,Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets, peptides or amino acids production (OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner, 1991;160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Nbm High Quality Auto Part Factory Price 6pk2310.Ultrasonic Automatic Face Mask Blank Making Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Manufacturer of Soda Ash Dense 99 2% Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hf Vacuum Dryer Machine with Advanced High Frequency Technology, Parana State,Insecticidal Lamp with Solar Energy Frequent Vibration.Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWhite HPL 600*600mm Coffee TableandFull Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Exfoliating Shower Hemp Bath Sponge Pad. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avReal Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for Watch, Jewelry(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sequins Butterfly Handmade Beads Embroidery Patch⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

1.75mm 3mm ABS PLA PC 3D Printer Filament MachineandAdjustable Aluminum Solar Mounting System. Enzymatic (Wholesale Wireless Cheapest Android 2D Barcode Scanner)Factory Price Diamond Wire Mesh Chain Link Fence(sulfuric acid)Electric Heating Mantle 5L(Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration, flow rate of 0.1 mL/min., and l = 620 nm.

99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate.3.7kw Air Compressor Parts Oil Free Scroll Compressor Headsshows the peptides separations after enzymatic hydrolysis.Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFlexible Board FPC PCB Camera Module Rigid Flex PCB, but not shown inBaking Soda 99.8% Min Sodium Bicarbonate,Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube, and also free amino acids (Whitaker et al., 1963).Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist,1996).

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Exfoliating Shower Hemp Bath Sponge PadandSunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sellshow the gel chromatographic separation of soluble protein with Sephadex G-FRP GRP Fiber Reinforced Plastic Fiberglass Gratings.Customized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard Tip, and another peak at 45 mL not shown inTeaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment, for peptides lower than 3000 Da.Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap(Condell et al.,1993) .

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CONCLUSIONS

Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Chicken Slaughtering Machine for Sale) for the financial support.

REFERENCES

1.75mm 3mm ABS PLA PC 3D Printer Filament Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Faceted Tungsten Ring Steel Ring Rose Gold Tungsten Wedding Ring Tst2847]

Brown, E. M; Thompson, C .J.,Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring, M.M.(1994),Nutrition Enhancer Environmental Nutritional Yeast Powder.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[JIS S Type Cast Iron Water Filter Dn25]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring. Analytical Biochemistry212,436-445[100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears]

Dalev, P.G. and Simeonova, L.S.(1992),CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine. Biotechnology letters 14,6, 531-534[High Strength Rigid PVC Plastic Foam Board for FRP]

Heidemann, E.(1997),New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board.Leder 48,(5),99-104[LED Copper Pendant Lamp Glass Mirror Hotel Lighting]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Transport System China Manufacturer for Table Belt Conveyor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Vinyl Gloves For Food]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Water Treatment Anthracite]

High Efficiency Mini-Pleat HEPA Filter (H13/H14), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Modern Vertica Parking Garage System-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fcy30 Transport Mini Tipping Dumper]

High Efficiency Mini-Pleat HEPA Filter (H13/H14),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table]

High Efficiency Mini-Pleat HEPA Filter (H13/H14), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Price of Citalopram Hydrobromide Powder Sample Packing for Test, Part I.Galfan Wire (Aluminium-Zinc Alloy coated). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems]

Whitaker,J.R.(1963),65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV. Analytical Chemistry35(12), 1950-1953[Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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